Phenotypic and functional characterization of human CD25⁺ B cells

Abstract

SummaryWe demonstrate that humans have a phenotypically and functionally distinct subset of B lymphocytes that express the interleukin (IL)-2 receptor (IL-2R) a-chain, cluster of differentiation (CD) 25. We found that one-third of the circulating CD20 + B cells expressed CD25 and, using

Epstein–Barr virus infection transforms CD25+ B cells into antibody‐secreting cells in rheumatoid arthritis patients

“…54 In patients with RA, the CD25 + B-cell subset belongs to the memory pool of B cells, which is functionally characterized by an increased IL-10 secretion and low spontaneous immunoglobulin secretion. [43][44][45] We found that the CD25 + B-cell population was enriched with the cells expressing the activation and apoptosis marker CD95. This is supported by our previous data where we observed that EBV replication gave rise to a concomitant expression of CD95 on CD19 + B cells and this might increase the sensitivity to RTX-induced depletion.…”

“…13,[43][44][45] The presence of EBV DNA was evaluated from the whole blood and BM aspirates using real-time PCR at the Virology Laboratory at Sahlgrenska University Hospital, Gothenburg, Sweden, as previously described. 25 Detection of 10 EBV-DNA copies was sufficient to stratify a patient as EBV + .…”

Specific Pathogen-Free Status Alters Immunophenotype in Rhesus Macaques: Implications for the Study of Simian Immunodeficiency Virus

“…55 CD25 + B cells are also described as having an activated phenotype with improved antigen presentation capabilities and the ability to trigger CD4 + T cell proliferation in mixed lymphocyte reaction cultures. 56 Alternately and depending on the cytokine environment, CD25 + B cells may be tolerogenic, participating in inhibition of the T cell proliferation and cytokine elaboration. 57 Similar to the increase in the memory T cell pool, a more mature B cell phenotype in standard SPF animals may be attributed to an early and recurring exposure to viral pathogens to which expanded SPF animals are not subject.…”

Adenosine production: a common path for mesenchymal stem-cell and regulatory T-cell-mediated immunosuppression

“…In addition to the search for alternative markers for identifying lymphocytes with suppressive potential, other lymphocyte populations with different phenotypes compared to the classical Tregs, have been described, including CD8+ regulatory T-cells [72], CD8+CD28− regulatory T-cells [73], CD3+CD4−CD8− regulatory T-cells (double negative) [74] and CD8+CXCR3+ regulatory T-cells [75]. Contributing to this already complex scenario, it was also shown that other immune cells possess capacity to control the immune response, such as regulatory B-cells [76] and myeloid-derived suppressor cells [77]. Recently, the interaction between these regulatory cells with Tregs began to be explored, leading to the observation that both regulatory B-cells and myeloid-derived suppressor cells act converting CD4 T-cells into Tregs [78,79].…”