Phenotypic and genetic consequences of size selection at the larval stage in the Pacific oyster (Crassostrea gigas)

Taris, Nicolas; Ernande, Bruno; McCombie, Helen; Boudry, Pierre

doi:10.1016/j.jembe.2005.12.007

Cited by 54 publications

(47 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The lower genetic diversity in the Northeast/ Hatcheries cluster (Table 1) was however expected as this has frequently been reported in hatchery shellfish seed (e.g. Gaffney et al 1992;Taris et al 2006;Hara and Sekino 2007;Lind et al 2009). The presence of two genetic clusters poses the question of the implications of the level of genetic diversity on their adaptive potential (i.e., invasion success).…”

Section: Discussionmentioning

confidence: 54%

Invasion genetics of the Pacific oyster Crassostrea gigas in the British Isles inferred from microsatellite and mitochondrial markers

et al. 2015

Self Cite

View full text Add to dashboard Cite

Abstract:The Pacific oyster, Crassostrea gigas, native to northeast Asia, is one of the most important cultured shellfish species. In Europe, Pacific oysters first settled along the Atlantic coasts of France at the end of the 1960s but rapidly spread and are now widely established. Twenty-two sites in the United Kingdom (UK), Ireland, Denmark, France and Spain were sampled to assess genetic diversity and differentiation. Hatcherypropagated stocks from two hatcheries located in the UK also were included. Two main genetic clusters were identified from pairwise genetic differentiation indexes, Bayesian clustering methods or neighbour-joining analysis, based on 7 microsatellite loci: (1) a Northeast cluster (including feral samples from East England, Ireland and Denmark as well as UK hatchery stocks) and (2) a Southwest cluster (including samples from South Wales, South West England, France and Spain). The Southwest cluster had significantly higher allelic richness (A) and expected heterozygosity (He) (A: 45.68, He: 0.928) than in the Northeast (A: 26.58, He: 0.883); the two diverging by a small but significant FST value (FST=0.017, 95% CI: 0.014-0.021). A 739-bp fragment of the major noncoding region of the mitochondrial genome was sequenced in 248 oysters from 12 of the studied samples in Europe and in 25 oysters from Miyagi prefecture (Japan). A total of 81 haplotypes were found. Haplotype frequency analyses identified the same two clusters observed using microsatellites. This study highlights how the number and size of introduction events, aquaculture practices, genetic bottlenecks followed by genetic drift and natural dispersal can act concurrently to shape the genetic diversity and structure of introduced populations.Response to Reviewers:The changes required by the reviewer have been made. The manuscript has been Powered by Editorial Manager® and ProduXion Manager® from Aries Systems Corporationsignificantly reduced in length. The figures 2, 3 and 5 have been modified following the reviewer's feedback.Line 199, we have specified that marine geographic distance corresponds to the nearest waterway distance.The deltaK method of Evanno has been used to determine the number of genetic clusters (see line 211). 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 2 Powered by Editorial Manager® and ProduXion Manager® from Aries Systems Corporation Abstract: 34 35The Pacific oyster, Crassostrea gigas, native to northeast Asia, is one of the most important cultured 36 shellfish species. In Europe, Pacific oysters first settled along the Atlantic coasts of France at the end 37 of the 1960s but rapidly spread and are now widely established. Twenty-two sites in the United 38 Kingdom (UK), Ireland, Denmark, France and Spain were sampled to assess genetic diversity and 39 differentiation. Hatchery-propagated stocks from two hatc...

show abstract

Section: Discussionmentioning

confidence: 54%

Invasion genetics of the Pacific oyster Crassostrea gigas in the British Isles inferred from microsatellite and mitochondrial markers

et al. 2015

Self Cite

View full text Add to dashboard Cite

show abstract

“…These results are not surprising because reduced genetic diversity has been reported in hatchery or aquaculture populations of finfish (Bouza et al 1997;Lundrigan H excess heterozygote excess method implemented in Colony v2.0 (Wang 2009), LD linkage disequilibrium method using LDNE program (Waples and Do 2008), Sibship sibship-based estimates using Colony v2.0 (Wang 2009), n sample size, N b effective breeding size, ? infinity 95% confidence intervals are given in brackets (Guillemin et al 2008) and shellfish (Gaffney et al 1992;Hedgecock et al 1992;Saavedra 1997;Taris et al 2006;Hara and Sekino 2007;Lind et al 2009). However, we are able to demonstrate that alternative managed reproduction methods using large scale ponds can retain high genetic diversity in the flat oyster and that this information is of significant value for restoration of this species.…”

Section: Loss Of Genetic Diversitymentioning

confidence: 99%

Strategies for the retention of high genetic variability in European flat oyster (Ostrea edulis) restoration programmes

et al. 2010

Self Cite

View full text Add to dashboard Cite

The native European flat oyster Ostrea edulis is listed in the OSPAR Convention for the Protection of the Marine Environment of the North-East Atlantic (species and habitat protection) and in the UK Biodiversity Action Plan. Once extremely abundant in the nineteenth century, European stocks of O. edulis have declined during the twentieth century to rare, small, localised populations due to overexploitation, habitat degradation and, most recently, the parasitic disease bonamiosis. Selective breeding programmes for resistance to bonamiosis have been initiated in France and Ireland. High genetic diversity and bonamiosis-resistance would be important features of any sustainable restoration programmes for O. edulis. Oysters were sampled across Europe from four hatchery sources, four pond-cultured sources and four wild, but managed fisheries and were genotyped at five microsatellite loci. Hatchery-produced populations from small numbers of broodstock showed a significant loss of genetic diversity relative to wild populations and pedigree reconstruction revealed that they were each composed of a single large full-sib family and several small full-sib families. This extremely low effective population size highlights the variance in reproductive success among the potential breeders. Pond-cultured oysters were intermediate in genetic diversity and effective population size between hatchery and wild populations. Controlled hatchery production allows the development of bonamiosis-resistant strains, but at the expense of genetic diversity. Large scale pond culture on the other hand can provide a good level of genetic diversity. A mixture of these two approaches is required to ensure a healthy and sustainable restoration programme for O. edulis in Europe.

show abstract

“…The culture of P. fucata martensii routinely involves hatchery practices such as mass spawning, use of uneven broodstock sex ratios and communal rearing of different families in Japan (Matsui 1965;Wada 1987). These practices have been shown to negatively impact levels of genetic variability and effective population sizes (Ne) in a variety of aquaculture species (Sekino et al 2003;Brown et al 2005;Taris et al 2006) and may affect P. fucata martensii populations of the present study in a similar fashion. Since it is well known that the pearl qualities such as the color and thickness of the nacreous layer are decided genetically Komaru 1990, 1996), most culturists carry out selective breeding of the oysters in order to produce good-quality pearls in Korea and Japan.…”

Section: Discussionmentioning

confidence: 55%

Genetic variation of hatchery and wild stocks of the pearl oyster Pinctada fucata martensii (Dunker, 1872), assessed by mitochondrial DNA analysis

Gwak

Nakayama

2011

Aquacult Int

View full text Add to dashboard Cite

In order to provide baseline information for the genetic resources, genetic variation in wild and cultured Pinctada fucata martensii from southern Korea and Japan was studied using nucleotide sequence analysis of 379 base pairs (bp) in the mitochondrial cytochrome oxidase subunit I gene (COI). The study included three hatchery stocks from Korea (Tongyeong) and Japan (Mie and Tsushima) and one wild stock from Korea (Geoje). A total of 3 haplotypes were identified in hatchery stocks of 78 individuals, of which 63 individuals shared 1 haplotype. Overall, nucleotide diversity (p) was low, ranging from 0.000 to 0.002, and haplotype diversity (h) ranged from 0.000 to 0.541. Considerably low haplotype and nucleotide diversities in hatchery stock indicated that low effective population size and consecutive selective breeding of P. fucata martensii could be responsible for the reduction in genetic variation. The wild stock exhibited low haplotype diversity (0.507 ± 0.039) with two shared haplotypes. The results of the present study with first record of wild pearl oyster in Korea support the possibility that the transplanted pearl oyster for overwintering experiments could have survived in winter. In order to enhance and/or maintain genetic diversity in the hatchery stock, further research should be directed toward genetic monitoring and evaluation of the hatchery and wild pearl oysters.

show abstract

Phenotypic and genetic consequences of size selection at the larval stage in the Pacific oyster (Crassostrea gigas)

Cited by 54 publications

References 39 publications

Invasion genetics of the Pacific oyster Crassostrea gigas in the British Isles inferred from microsatellite and mitochondrial markers

Invasion genetics of the Pacific oyster Crassostrea gigas in the British Isles inferred from microsatellite and mitochondrial markers

Strategies for the retention of high genetic variability in European flat oyster (Ostrea edulis) restoration programmes

Genetic variation of hatchery and wild stocks of the pearl oyster Pinctada fucata martensii (Dunker, 1872), assessed by mitochondrial DNA analysis

Contact Info

Product

Resources

About