Phenotypic and genotypic chactracterization of Campylobacter isolated from poultry

Abstract

Campylobacteriosis incidence and proportion of Campylobacter strains resistant to antibiotic have been increased worldwide in the last decades. Campylobacteriosis is considered as one of the major important zoonotic gastrointestinal diseases around the world. The aim of this study was isolation and biochemical characterization of Campylobacter from poultry. Campylobacter isolation have been done on MCCDA media, motility has been detected by phase contrast microscope, morphology has been detected by Gram stain, and confirmed by MALDI-TOF MS and molecular confirmation by using cPCR by amplification of 16S rRNA gene. A total of 102 isolates of Campylobacter were isolated from farm and small backyards located in different sources in El-Kalyobia , El-Monofia and El-sharkia Governorates by conventional bacteriological methods from which 40 were C. jejuni and 62 were C. coli. All isolates have been confirmed as Campylobacter by MALDI-TOF MS and detection of 16S rRNA by cPCR. PCR is a useful molecular tool for identification and confirmation of Campylobacter. It is rapid, sensitive, and specific than the culture methods, but the only disadvantage is that is expensive.

The role of probiotic in the reduction of the colonization of Campylobacter jejuni in broiler chickens

“…Among the examined C. jejuni isolates, the cytolethal distending toxin (CDT) cluster genes cdtA, cdtB, and cdtC accounted for the majority of virulence genes (85.7%). These findings were published in multiple researches by Krutkiewicz et al (2010) andAhmed et al (2019).…”