Phenotypic and genotypic characterization of rhizobia associated with Acacia saligna (Labill.) Wendl. in nurseries from Algeria

Amrani, Saïd; Noureddine, Nazhat-Ezzaman; Bhatnagar, Tej; Argandoña, Montserrat; Nieto, Joaquı́n J.; Vargas, Carmen Regla

doi:10.1016/j.syapm.2009.09.003

Cited by 31 publications

(37 citation statements)

References 41 publications

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“…Data evidenced a distribution of rhizobial strains according to the geographic location in Senegal. Similar results were found by Amrani et al (2010) on Acacia saligna plants in Algerian soils which were nodulated by different rhizobial populations according to their geographical origin (i.e. Northern vs Southern areas).…”

Section: Distribution Of Rhizobial Strains In the Two Regionssupporting

confidence: 85%

Distribution and Diversity of Rhizobial Populations Associated with <i>Acacia senegal</i> (L.) Willd. Provenances in Senegalese Arid and Semiarid Regions

Bakhoum¹,

Roux²,

Diouf³

et al. 2014

OJF

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Distribution and diversity of rhizobial strains associated with Acacia senegal (L.) Willd. in relation to seed provenances in soils from arid (Dahra) and semiarid (Goudiry) zones of Senegal were investigated. PCR-RFLP performed on 16S-23S rDNA intergenic spacer (IGS) of nodule crude extracts revealed a high genetic diversity of rhizobial strains, which was higher in the semiarid region than in the arid region. The distribution of rhizobial populations was influenced by soil physical and chemical characteristics, and by A. senegal provenances as shown by the analysis of correspondence. In contrast, the phenotypic diversity of rhizobial strains was not correlated with the soil origin. The phylogenetic tree (performed by the maximum likelihood algorithm) of IGS 16S-23S sequences showed that most of the rhizobial strains nodulating A. senegal were closely related to Mesorhizobium plurifarium. Our results showed that rhizobial taxa associated with A. senegal were mainly distributed according to soil physical and chemical characteristics, and A. senegal provenances. A large subset of A. senegal root-nodulating bacteria had high diversity that correlated with the most favourable environmental conditions. Understanding the diversity and distribution of rhizobial strains may be exploited in the formulation of A. senegal inoculants for different seed provenances for resilience to soil stresses in various environmental conditions.

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Section: Distribution Of Rhizobial Strains In the Two Regionssupporting

confidence: 85%

Distribution and Diversity of Rhizobial Populations Associated with <i>Acacia senegal</i> (L.) Willd. Provenances in Senegalese Arid and Semiarid Regions

Bakhoum¹,

Roux²,

Diouf³

et al. 2014

OJF

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“…Such promiscuity has rarely been reported in other legume species. Other studies also showed that A. saligna was nodulated either by fast‐ and slow‐growing rhizobia, either with a predominance of slow‐growing ones (Marsudi et al ., ) or in equal proportions (Amrani et al ., ) but always with a lower diversity than in our survey. Partial sequencing of the 16S rRNA gene does not allow the identification of Bradyrhizobium taxa to the species level, and at least 16S‐23S rRNA‐ITS gene sequencing is requested (Willems, ).…”

Section: Discussioncontrasting

confidence: 66%

Symbiotic characterization and diversity of rhizobia associated with native and introduced acacias in arid and semi-arid regions in Algeria

Boukhatem

Domergue

Bekki

et al. 2012

FEMS Microbiol Ecol

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The diversity of rhizobia associated with introduced and native Acacia species in Algeria was investigated from soil samples collected across seven districts distributed in arid and semi-arid zones. The in vitro tolerances of rhizobial strains to NaCl and high temperature in pure culture varied greatly regardless of their geographical and host plant origins but were not correlated with the corresponding edaphoclimatic characteristics of the sampling sites, as clearly demonstrated by principal component analysis. Based on 16S rRNA gene sequence comparisons, the 48 new strains isolated were ranked into 10 phylogenetic groups representing five bacterial genera, namely, Ensifer, Mesorhizobium, Rhizobium, Bradyrhizobium, and Ochrobactrum. Acacia saligna, an introduced species, appeared as the most promiscuous host because it was efficiently nodulated with the widest diversity of rhizobia taxa including both fast-growing ones, Rhizobium, Ensifer, and Mesorhizobium, and slow-growing Bradyrhizobium. The five other Acacia species studied were associated with fast-growing bacterial taxa exclusively. No difference in efficiency was found between bacterial taxa isolated from a given Acacia species. The tolerances of strains to salinity and temperature remains to be tested in symbiosis with their host plants to select the most adapted Acacia sp.-LNB taxa associations for further revegetation programs.

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“…Based on the results of the symbiotic effectiveness tests conducted in a Gibson tube experiment and phenotypic characterization, the 17 most representative isolates: S 4 F(3) T , S 5 F(I), S 7 F(3), S 8 TL 2 , S 8 T T2 tr, S 9 F(II), S 10 F(I), S 10 F(II), S 11 F 1 (I), S 11 F(III), S 13 F 3 (I), S 13 PC(6), S 14 F1(I), S 14 F 7 (II), S 14 F 10 (II), S 14 F 10 (III), and S 15 a 2 were chosen for further genotypic characterization. The 16S rDNA were amplified and a single band of about 1500 bp for all the strains was obtained (Figure 2), which corresponded to the expected size of the 16S rRNA gene (Amrani et al, 2010).…”

Section: Genotypic Characterizationmentioning

confidence: 86%

Phenotypic and genotypic characterizations of rhizobia isolated from root nodules of multiple legume species native of Fez, Morocco

Berrada¹,

Nouioui²,

Houssaini³

et al. 2012

Afr. J. Microbiol. Res.

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Root-nodulating bacteria were isolated and characterized from grain and forage legumes growing in Fez regions. A total of 110 Rhizobium strains were characterized on the basis of morphological, cultural, and phenotypical properties. Phenotypic characteristics studied included growth rates in various media, colony morphology, tolerances to extremes of temperature, salt and pH and resistance to different concentrations of heavy metals and antibiotics. The isolates were very diverse phenotypically. Eighty seven of isolates were fast growing rhizobia. Thirty four strains tolerated high concentration of salt (2% NaCl). The majority of our isolates tolerated temperatures ranging between 20 and 37°C, but not above 55°C. Also, the isolated strains tolerated extreme pH in their medium from 4.8 to 8.8. The antibiotic resistance of these strains showed a high level of resistance against streptomycin, kanamycin, ampicillin, tetracycline, and chloramphenicol. The highest resistance to heavy metals was recorded for arsenate, copper, zinc, nickel, and mercury. Also, a moderate resistance was found for chromium. Polymerase chain reaction (PCR) method and 16S rRNA gene sequencing were used for the genotypic analysis of 17 Rhizobium strains selected on the basis of phenotypic study. The results showed a high diversity among the strains.

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Phenotypic and genotypic characterization of rhizobia associated with Acacia saligna (Labill.) Wendl. in nurseries from Algeria

Cited by 31 publications

References 41 publications

Distribution and Diversity of Rhizobial Populations Associated with <i>Acacia senegal</i> (L.) Willd. Provenances in Senegalese Arid and Semiarid Regions

Distribution and Diversity of Rhizobial Populations Associated with <i>Acacia senegal</i> (L.) Willd. Provenances in Senegalese Arid and Semiarid Regions

Symbiotic characterization and diversity of rhizobia associated with native and introduced acacias in arid and semi-arid regions in Algeria

Phenotypic and genotypic characterizations of rhizobia isolated from root nodules of multiple legume species native of Fez, Morocco

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Phenotypic and genotypic characterization of rhizobia associated with Acacia saligna (Labill.) Wendl. in nurseries from Algeria

Cited by 31 publications

References 41 publications

Distribution and Diversity of Rhizobial Populations Associated with &lt;i&gt;Acacia senegal&lt;/i&gt; (L.) Willd. Provenances in Senegalese Arid and Semiarid Regions

Distribution and Diversity of Rhizobial Populations Associated with &lt;i&gt;Acacia senegal&lt;/i&gt; (L.) Willd. Provenances in Senegalese Arid and Semiarid Regions

Symbiotic characterization and diversity of rhizobia associated with native and introduced acacias in arid and semi-arid regions in Algeria

Phenotypic and genotypic characterizations of rhizobia isolated from root nodules of multiple legume species native of Fez, Morocco

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Distribution and Diversity of Rhizobial Populations Associated with <i>Acacia senegal</i> (L.) Willd. Provenances in Senegalese Arid and Semiarid Regions

Distribution and Diversity of Rhizobial Populations Associated with <i>Acacia senegal</i> (L.) Willd. Provenances in Senegalese Arid and Semiarid Regions