Phenotypic and genotypic characterization of antibiotic-resistant Propionibacterium acnes isolated from acne patients attending dermatology clinics in Europe, the U.S.A., Japan and Australia

Abstract

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In vitro antimicrobial susceptibility patterns of Propionibacterium acnes isolated from patients with acne vulgaris

“…There is widespread resistance in P. acnes due to overuse of topical and systemic antibiotics for treatment of acne vulgaris [3,[8][9][10][11][12][13][14][15][16], as shown in Table 4. Various studies have used different interpretative criteria to estimate the resistance among P. acne strains to different anti-acne drugs.…”

“…Pathogenesis of acne is a complex interplay of inflammation, hyperkeratinisation of the sebaceous duct, high sensitivity to circulating androgens, and bacterial colonization [2]. Topical antibiotics such as erythromycin, clindamycin, and tetracycline are routinely used for long-term treatment of acne vulgaris, which exerts considerable selective pressure for the development of drug resistance [3]. This study was undertaken to examine the bacteriological profile of acne vulgaris and to ascertain its antimicrobial resistance patterns.…”

Emergence of Tetracycline Resistance in Helicobacter pylori : Multiple Mutational Changes in 16S Ribosomal DNA and Other Genetic Loci

“…Resistance to tetracycline is common in many bacterial species. In Propionibacterium acnes this resistance is ascribed to a mutation affecting one specific position in the tetracycline binding site (position G1058 in rRNA helix 34) in a remarkable 85% of tetracycline-resistant (Tet r ) clinical isolates (34,35). In most other species, however, resistance tends to be conferred by auxiliary genes carried on plasmids, transposons, or other mobile DNA elements and typically involves proteins that variously (i) promote tetracycline efflux, (ii) bind to and change the 30S ribosomal subunit conformation and thereby interfere with tetracycline-ribosome binding, or (iii) cause enzymatic inactivation of the drug (9).…”

“…Thus, unlike drug resistance in many pathogens, resistance in H. pylori usually involves only mutant alleles of normal chromosomal genes, not mobile DNA element-borne auxiliary resistance determinants. Third, tetracycline resistance in P. acnes is often due to a mutation in the tetracycline binding pocket in 16S rRNA (34,35), as noted above.…”

A microbiological approach to acne vulgaris.

“…Other antibiotic agents are also being applied (macrolides, lincosamides), in dependence on the limits for tetracycline indication and according to the verified therapy used by various centers. Sulfonamides are utilized in the acne therapy too, but the authors' opinions on their use in this indication are different 7,8,11,15,24,26,31,32,45,49 . Hormones are also effective in the treatment of acne.…”