Phenotypic and Genotypic Characterization of Virulence Factors and Susceptibility to Antibiotics in Salmonella Infantis Strains Isolated from Chicken Meat: First Findings in Chile

Lapierre, Lisette; Cornejo, Javiera; Zavala, Sebastián; Galarce, Nicolás; Sánchez, Francisco Javier Sierro; Benavides, María Belén; Guzmán, Miguel; Sáenz, Leonardo

doi:10.3390/ani10061049

Cited by 41 publications

(42 citation statements)

References 57 publications

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“…The presence of Salmonella strains was mainly observed in serogroups B and C1 within different animal populations in Chile. Coincident with our study, other studies carried out in Chile have found these common serovars to be Enteritidis, Typhimurium and Infantis (serogroup D, B and C1) [ 15 , 18 , 22 , 24 , 27 , 28 , 29 , 30 ]. However, S. Enteritidis has represented the highest percentage of isolates [ 15 , 16 , 17 , 18 , 19 , 22 ].…”

Section: Discussionsupporting

confidence: 90%

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Screening the Presence of Non-Typhoidal Salmonella in Different Animal Systems and the Assessment of Antimicrobial Resistance

Rivera

Allel

Dueñas

et al. 2021

Animals

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Salmonella is a major bacterial foodborne pathogen that causes the majority of worldwide food-related outbreaks and hospitalizations. Salmonellosis outbreaks can be caused by multidrug-resistant (MDR) strains, emphasizing the importance of maintaining public health and safer food production. Nevertheless, the drivers of MDR Salmonella serovars have remained poorly understood. In this study, we compare the resistance profiles of Salmonella strains isolated from 4047 samples from domestic and wild animals in Chile. A total of 106 Salmonella strains (2.61%) are isolated, and their serogroups are characterized and tested for susceptibility to 16 different antimicrobials. The association between antimicrobial resistance (AMR) and a subset of independent variables is evaluated using multivariate logistic models. Our results show that 47 antimicrobial-resistant strains were found (44.3% of the total strains). Of the 47, 28 correspond to single-drug resistance (SDR = 26.4%) and 19 are MDR (17.9%). S. Enteritidis is highly persistent in animal production systems; however, we report that serogroup D strains are 18 times less likely to be resistant to at least one antimicrobial agent than the most common serogroup (serogroup B). The antimicrobials presenting the greatest contributions to AMR are ampicillin, streptomycin and tetracycline. Additionally, equines and industrial swine are more likely to acquire Salmonella strains with AMR. This study reports antimicrobial-susceptible and resistant Salmonella in Chile by expanding the extant literature on the potential variables affecting antimicrobial-resistant Salmonella.

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Section: Discussionsupporting

confidence: 90%

“…[ 29 ]. Similarly to this report, a previous article [ 30 ] also indicated a high contamination by S. Infantis of chicken meat available for consumption in supermarkets. However, a low prevalence is reported in backyard poultry farms (approximately 1%) [ 15 ].…”

Section: Discussionsupporting

confidence: 81%

Screening the Presence of Non-Typhoidal Salmonella in Different Animal Systems and the Assessment of Antimicrobial Resistance

Rivera

Allel

Dueñas

et al. 2021

Animals

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show abstract

“…Lower resistance to tetracycline and chloramphenicol occurred in this study. In Chile, Salmonella infantis from chicken meat was resistant to amoxycillin/clavulanic acid (3.5%), azithromycin (2.3%), ceftriaxone (67.8%), chloramphenicol (64.4%), ciprofloxacin (2.3%), gentamycin (11.5%), tetracycline (95.4%), and sulfamethoxazole/trimethoprim (49.4%) [ 44 ]. This study found higher resistance to amoxycillin/clavulanic acid, azithromycin, and gentamycin, but lower for the rest of the antibiotics tested.…”

Section: Discussionmentioning

confidence: 99%

Not All Street Food Is Bad: Low Prevalence of Antibiotic-Resistant Salmonella enterica in Ready-to-Eat (RTE) Meats in Ghana Is Associated with Good Vendors’ Knowledge of Meat Safety

Aduah

Adzitey

Amoako

et al. 2021

Foods

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Foodborne infections due to the consumption of meat is a significant threat to public health. However, good vendor and consumer knowledge of meat safety could prevent meat contamination with and transmission of foodborne pathogens like Salmonella. Thus, this study investigated the vendor and consumer perception, knowledge, and practices of meat safety regarding ready-to-eat (RTE) meat and how this affected the prevalence and antibiotic susceptibility of Salmonella enterica in RTE meats in the streets of Ghana. A semi-structured questionnaire was used to obtain the demographics, knowledge, and practices of meat safety data from RTE meat vendors (n = 300) and consumers (n = 382). Salmonella enterica detection was done according to the United State of America (USA)-Food and Drugs Administration (FDA) Bacteriological Analytical Manual. The disk diffusion method was used for antibiotic resistance testing. The results revealed that most of the respondents had heard of meat safety (98.3% vendors, 91.8% consumers) and knew that meat could be contaminated by poor handling (100.0% vendors, 88.9% consumers). The respondents knew that regular hand washing reduced the risk of meat contamination (100.0% vendors, 94.0% consumers). Responses to the practices of meat safety by vendors were generally better. A very low Salmonella enterica prevalence was observed in the samples, ranging between 0.0 and 4.0% for guinea fowl and beef, respectively. However, the six isolates obtained were resistant to five of the nine antibiotics tested, with all isolates displaying different resistance profiles. Overall, the good knowledge and practice of meat safety demonstrated by the respondents corroborated the negligible prevalence of Salmonella in this study, reiterating the importance of vendor meat safety knowledge. However, the presence of resistant Salmonella enterica in some of the meat samples, albeit in a very low prevalence, warrants stricter sanitary measures and greater meat safety awareness in the general population to prevent meat-borne infections and potential transmission of drug-resistant bacteria to humans.

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“…Nevertheless, a recent publication from Chile reported that 24% of broiler meat samples ( n = 361) were positive to the isolation of S . Infantis ( 61 ). This data highlights the necessity of more research in the field to better understand the epidemiology of Salmonella in the region.…”

Section: Discussionmentioning

confidence: 99%

Genomic Epidemiology of Salmonella Infantis in Ecuador: From Poultry Farms to Human Infections

et al. 2020

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Salmonella enterica is one of the most important foodborne pathogens around the world. In the last years, S . enterica serovar Infantis has become an important emerging pathogen in many countries, often as multidrug resistant clones. To understand the importance of S. enterica in the broiler industry in Ecuador, we performed a study based on phenotypic and WGS data of isolates from poultry farms, chicken carcasses and humans. We showed a high prevalence of S. enterica in poultry farms (41.4%) and chicken carcasses (55.5%), but a low prevalence (1.98%) in human samples. S . Infantis was shown to be the most prevalent serovar with a 98.2, 97.8, and 50% in farms, foods, and humans, respectively, presenting multidrug resistant patterns. All sequenced S . Infantis isolates belonged to ST32. For the first time, a pESI-related megaplasmid was identified in Ecuadorian samples. This plasmid contains genes of antimicrobial resistance, virulence factors, and environmental stress tolerance. Genomic analysis showed a low divergence of S . Infantis strains in the three analyzed components. The results from this study provide important information about genetic elements that may help understand the molecular epidemiology of S . Infantis in Ecuador.

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Phenotypic and Genotypic Characterization of Virulence Factors and Susceptibility to Antibiotics in Salmonella Infantis Strains Isolated from Chicken Meat: First Findings in Chile

Cited by 41 publications

References 57 publications

Screening the Presence of Non-Typhoidal Salmonella in Different Animal Systems and the Assessment of Antimicrobial Resistance

Screening the Presence of Non-Typhoidal Salmonella in Different Animal Systems and the Assessment of Antimicrobial Resistance

Not All Street Food Is Bad: Low Prevalence of Antibiotic-Resistant Salmonella enterica in Ready-to-Eat (RTE) Meats in Ghana Is Associated with Good Vendors’ Knowledge of Meat Safety

Genomic Epidemiology of Salmonella Infantis in Ecuador: From Poultry Farms to Human Infections

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