Phenotypic and Genotypic Detection of Efflux Pump Mediated Meropenem Resistance in Pseudomonas aeruginosa isolates from Catheter Associated Urinary Tract Infection

Khalek, Hanan Abdel; Ramadan, Mona; Radwan, Mohamed M.

doi:10.21608/ejmm.2020.249871

Cited by 3 publications

(1 citation statement)

References 18 publications

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“…The mean MIC of meropenem/ imipenem negative efflux pump phenotype was significantly increased as compared to the MIC with positive efflux pump phenotype (p<0.001). This was consistent with Kishk et al 21 and Khalek et al 22 who discovered that the primary mechanism underlying carbapenem resistance was efflux pump activation, which was phenotypically validated by the addition of CCCP as a pump inhibitor. This also resembled the outcomes of Choudhury et al 23 who had observed phenotypic efflux pump activity and a significant drop in MIC in all of their isolates of P. aeruginosa upon addition of CCCP.…”

Section: Discussionsupporting

confidence: 89%

Detection of Efflux Pumps in Carbapenem Resistant Pseudomonas Aeruginosa Isolated from Benha University Hospitals

Elmosallamy,

Tabl,

Ghanem

et al. 2024

Egyptian Journal of Medical Microbiology

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Background: Pseudomonas aeruginosa (P. aeruginosa) infections pose a significant risk to hospitalized patients due to its high prevalence and inherent resistance mechanisms. Efflux pumps, particularly the Mex family, contribute significantly to multidrug resistance (MDR) in P. aeruginosa. Objectives: To detect phenotypic and genotypic efflux pump-mediated antibiotic resistance in P. aeruginosa isolated from patients admitted to Benha University Hospitals. Methodology: This cross-sectional descriptive study was executed on 50 P. aeruginosa strains isolated from different clinical samples of patients admitted to hospitals of Benha University. The isolates underwent identification and antimicrobial susceptibility testing by means of VITEK® 2 compact system. The phenotypic identification of the efflux pumps was done by the Carbonyl Cyanide M-Chlorophenyl Hydrazone test (CCCP Test), followed by identification of different genes encoding efflux pump-mediated carbapenem resistance by PCR assays. Results: from the different clinical samples, P. aeruginosa were isolated mostly from sputum samples (30%), wound swabs (24%) and urine samples (22%). P. aeruginosa isolates exhibited varying sensitivities to tested antibiotics, showing the highest sensitivity to colistin (70%). The Minimum Inhibitory Concentration (MIC) of meropenem and imipenem significantly decreased in the existence of CCCP, indicating efflux pump involvement. PCR results revealed a high prevalence of MexA (72.4%) and MexE (79.3%) genes, correlating well with phenotypic findings. Conclusion: Efflux pumps have a significant function in mediating antibiotic resistance in P. aeruginosa isolates. The correlation between phenotypic and genotypic results, particularly the prevalence of MexA and MexE genes, underscores the importance of efflux pumps in MDR in P. aeruginosa strains from this clinical setting.

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Section: Discussionsupporting

confidence: 89%

Detection of Efflux Pumps in Carbapenem Resistant Pseudomonas Aeruginosa Isolated from Benha University Hospitals

Elmosallamy,

Tabl,

Ghanem

et al. 2024

Egyptian Journal of Medical Microbiology

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Phenotypic and genotypic assessment of fluoroquinolones and aminoglycosides resistances in Pseudomonas aeruginosa collected from Minia hospitals, Egypt during COVID-19 pandemic

Boushra,

Gad,

Hassuna

et al. 2024

BMC Infect Dis

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Background One of the most prevalent bacteria that cause nosocomial infections is Pseudomonas aeruginosa. Fluoroquinolones (FQ) and aminoglycosides are vital antipseudomonal drugs, but resistance is increasingly prevalent. The study sought to investigate the diverse mechanisms underlying FQ and aminoglycoside resistance in various P. aeruginosa strains particularly during the COVID-19 crisis. Methods From various clinical and environmental samples, 110 P. aeruginosa isolates were identified and their susceptibility to several antibiotic classes was evaluated. Molecular techniques were used to track target gene mutations, the presence of genes encoding for quinolone resistance, modifying enzymes for aminoglycosides and resistance methyltransferase (RMT). Efflux pump role was assessed phenotypically and genotypically. Random amplified polymorphic DNA (RAPD) analysis was used to measure clonal diversity. Results QnrS was the most frequently encountered quinolone resistance gene (37.5%) followed by qnrA (31.2%) and qnrD (25%). Among aminoglycoside resistant isolates, 94.1% harbored modifying enzymes genes, while RMT genes were found in 55.9% of isolates. The aac(6')-Ib and rmtB were the most prevalent genes (79.4% and 32.3%, respectively). Most FQ resistant isolates overexpressed mexA (87.5%). RAPD fingerprinting showed 63.2% polymorphism. Conclusions Aminoglycosides and FQ resistance observed in this study was attributed to several mechanisms with the potential for cross-contamination existence so, strict infection control practices are crucial.

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Effect of small-interfering RNA (As1974) and HFq-binding proteins on resistance gene and host microRNA (miR-30C) expression in Pseudomonas aeruginosa-infected patients from Iraq

Al fatlah,

Bunyan,

Al Husseini

2023

MJBL

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Background: MicroRNAs (miRNAs) are a class of small RNAs encoded by the genome that regulate the production of cellular mRNAs that include either incomplete or complete miRNA-binding sites. Objectives: To evaluate the impact of sRNA (As1974) and HFq-binding proteins on the expression of resistance gene and host miRNA (miR-30C) in Iraqi urinary tract infections (UTIs) patients infected with Pseudomonas aeruginosa. Materials and Methods: Patients with UTIs from Baghdad, Iraq’s Baghdad Teaching Hospital, Ghazi Hariri Hospital, Central Laboratories in Medical City, and Al-Yarmouk Hospital were recruited during June 2022 and October 2022 to provide 200 clinical samples. Results: Out of 200 patients with UTIs, only 56 (38.14%) were diagnosed as P. aeruginosa from positive urine samples. Urine samples were analyzed for HFQ gene expression, and the results showed that HFQ is overexpressed in P. aeruginosa-resistant samples compared to sensitive clinical samples, as measured by fold change after normalization with housekeeping gene 16sRNA by folding (21.4971.241 vs. 1.92142 0.04598). Furthermore, normalization of As1974 gene expression in urine samples using 16sRNA revealed a downregulation of As1974 in resistance, with a fold change of 0.66220.0465 versus 2.0121.0243. The miR-30 gene was shown to be downregulated in urine and blood samples (1.360.34, 0.478210.03678) as compared to those of healthy subjects. Conclusions: All ages were susceptible to the UTIs, also females suffered from UTIs more than males. A significant over expression of HFQ-binding protein in P. aeruginosa compared to sensitive clinical samples. Downregulation of As1974 in resistance.

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Phenotypic and Genotypic Detection of Efflux Pump Mediated Meropenem Resistance in Pseudomonas aeruginosa isolates from Catheter Associated Urinary Tract Infection

Cited by 3 publications

References 18 publications

Detection of Efflux Pumps in Carbapenem Resistant Pseudomonas Aeruginosa Isolated from Benha University Hospitals

Detection of Efflux Pumps in Carbapenem Resistant Pseudomonas Aeruginosa Isolated from Benha University Hospitals

Phenotypic and genotypic assessment of fluoroquinolones and aminoglycosides resistances in Pseudomonas aeruginosa collected from Minia hospitals, Egypt during COVID-19 pandemic

Effect of small-interfering RNA (As1974) and HFq-binding proteins on resistance gene and host microRNA (miR-30C) expression in Pseudomonas aeruginosa-infected patients from Iraq

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