Phenotypic and genotypic identification of <i>Candida dubliniensis</i> from subgingival sites in immunocompetent subjects in Argentina

Jewtuchowicz, Virginia; Mujica, María Teresa; Brusca, María Isabel; Sordelli, N.; Malzone, Maria Celina; Pola, S. J.; Iovannitti, C.; Rosa, Alcira C

doi:10.1111/j.1399-302x.2008.00465.x

Cited by 40 publications

(39 citation statements)

References 30 publications

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“…albicans was the most commonly identified Candida species in this group of patients with periodontitis, which is in agreement with data from several previous studies (1,5,8,10,32,35). This species was most commonly recovered from 10/21 (47.6%) periodontitis patients both by oral rinse sampling and by the sampling of periodontal pockets using both paper points and curettes.…”

Section: Discussionsupporting

confidence: 79%

“…Selected isolates of each colony color and morphology were purified by subculturing for further analysis. Isolates were initially presumptively identified on the basis of colony color and morphology: C. albicans isolates were distinguished from C. dubliniensis isolates on the basis of the darker blue-green appearance and smaller colony size of C. dubliniensis, in contrast to the larger, paler green colonies formed by C. albicans upon primary isolation in this medium (10). Definitive identification was undertaken by determining Candida isolate substrate assimilation profiles using the API ID 32C yeast identification system (bioMérieux, Marcy l'Etoile, France), as described previously (13).…”

Section: Methodsmentioning

confidence: 99%

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Enrichment of Multilocus Sequence Typing Clade 1 with Oral Candida albicans Isolates in Patients with Untreated Periodontitis

et al. 2012

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cThis study investigated the prevalence and cell density of Candida species in periodontal pockets, healthy subgingival sites, and oral rinse samples of patients with untreated periodontitis. Twenty-one periodontitis patients underwent sampling at two periodontitis sites, and 19/21 of these patients underwent sampling at one periodontally healthy site. Both paper point and curette sampling techniques were employed. The periodontitis patients and 50 healthy subjects were also sampled by oral rinse. Candida isolates were recovered on CHROMagar Candida medium, and representative isolates were identified. Candida spp. were recovered from 10/21 (46.7%) periodontitis patients and from 16/50 (32%) healthy subjects. C. albicans predominated in both groups and was recovered from all Candida-positive subjects. Candida-positive periodontitis patients yielded Candida from periodontal pockets with average densities of 3,528 and 3,910 CFU/sample from curette and paper point samples, respectively, and 1,536 CFU/ml from oral rinse samples. The majority (18/19) of the healthy sites sampled from periodontitis patients were Candida negative. The 16 Candida-positive healthy subjects yielded an average of 279 CFU/ml from oral rinse samples. C. albicans isolates were investigated by multilocus sequence typing (MLST) to determine if specific clonal groups were associated with periodontitis. MLST analysis of 31 C. albicans isolates from periodontitis patients yielded 19 sequence types (STs), 13 of which were novel. Eleven STs belonged to MLST clade 1. In contrast, 16 C. albicans isolates from separate healthy subjects belonged to 16 STs, with 4 isolates belonging to clade 1. The distributions of STs between both groups were significantly different (P ‫؍‬ 0.04) and indicated an enrichment of C. albicans isolates in periodontal pockets, which warrants a larger study.

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Section: Discussionsupporting

confidence: 79%

Section: Methodsmentioning

confidence: 99%

Enrichment of Multilocus Sequence Typing Clade 1 with Oral Candida albicans Isolates in Patients with Untreated Periodontitis

et al. 2012

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“…With a long-term course of chronic inflammatory processes in the oral cavity and a wasteful long-term treatment of patients with periodontal pathology using synthetic antibiotics and local antiseptics, an imbalance develops between different types of fungalbacterial associations, which are accompanied with the emergence of oral cavity dismicrobiocenosis [4]. The prevalence of the active colonization of yeast-like fungi of Candida type in periodontal pockets of patients with chronic periodontitis is in 40% to 70% of cases [5,6]. At the moment, the actual question remains about the impact of personal oral cavity hygiene products that contain antiseptics to the composition of the microbiota and the local immune status of the oral cavity.…”

Section: Introductionmentioning

confidence: 99%

Immunological and Microbiological Aspects of the Effectiveness of Oral Hygiene for Patients With Periodontal Inflammatory Diseases

Ovcharenko¹,

Еричев²,

Аксенова³

et al. 2017

RAD Conference Proceedings

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Abstract. The research objective consisted in studying the local factors of protection of an oral cavity on the basis of balance definition between proinflammatory (INf -γ, TNF -α, IL-8) and anti-inflammatory (IL-4) cytokines with the degree of colonization of Candida in patients with chronic generalized parodontitis. On the basis of the results of this study, it has been established that patients with chronic parodontitis who have a mild and moderate severity level of disease, a high degree colonization of periodontal pockets with Candida tropicalis correlates with a substantial increase in proinflammatory cytokines (TNF -α, IL-8), concentration fall of INF -γ and the increase in antiinflammatory cytokines of IL-4 level.

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“…Although the yeast most frequently associated to this type of infection is Candida albicans , other less prevalent emerging species have been isolated (4, 6, 7, 9–12). …”

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confidence: 99%

“…Colonization of the oral cavity appears to be facilitated by several specific adherence interactions between Candida species and oral surfaces which enable the yeast to resist host clearance mechanisms. Mucosal and subgingival Candida dubliniensis colonization has been reported in immunocompetent subjects with periodontal disease (6, 7, 14) and both oral sites are appropriate niches for multiplication of this species.…”

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confidence: 99%

Genetic relatedness of subgingival and buccalCandida dubliniensisisolates in immunocompetent subjects assessed by RAPD-PCR

Jewtuchowicz

Mujica

Malzone

et al. 2009

Journal of Oral Microbiology

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BackgroundIt is recognized that Candida dubliniensis commonly colonizes oral and subgingival sites in immunocompetent subjects with periodontal disease.ObjectiveSince there are few data available on genetic characterization of C. dubliniensis in periodontal pockets and other oral sites, the aim of this study was to characterize subgingival and mucosal C. dubliniensis isolates recovered from immunocompetent subjects and to assay the genetic similarity of such isolates from both niches in the same patient by random amplified polymorphic DNA (RAPD).Design C. dubliniensis recovered from subgingival plaque and from buccal cavity samples were studied in 240 immunocompetent non-smoking individuals. Arbitrary amplification was carried out by RAPD-polymerase chain reaction (PCR).ResultsRAPD analysis showed identical genotypes of C. dubliniensis in different sampling sites (buccal cavity and subgingival areas) in eight of 10 patients except for those derived from two participants who presented presumably unrelated isolates.ConclusionsOn the basis of the findings presented, the origin of the colonization of C. dubliniensis in subgingival biofilm seems to be the buccal cavity in a single patient. Consequently, it may be assumed that most of C. dubliniensis in these sites arise from the endogenous commensal strains.

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Phenotypic and genotypic identification of Candida dubliniensis from subgingival sites in immunocompetent subjects in Argentina

Cited by 40 publications

References 30 publications

Enrichment of Multilocus Sequence Typing Clade 1 with Oral Candida albicans Isolates in Patients with Untreated Periodontitis

Enrichment of Multilocus Sequence Typing Clade 1 with Oral Candida albicans Isolates in Patients with Untreated Periodontitis

Immunological and Microbiological Aspects of the Effectiveness of Oral Hygiene for Patients With Periodontal Inflammatory Diseases

Genetic relatedness of subgingival and buccalCandida dubliniensisisolates in immunocompetent subjects assessed by RAPD-PCR

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