Phenotypic and genotypic profile of pyrethroid resistance in populations of the mosquito Aedes aegypti from Goiânia, Central West Brazil

Chapadense, Francesca Guaracyaba Garcia; Fernandes, Éverton K. K.; Lima, José Bento Pereira; Martins, Ademir Jesús; Silva, Luana Carrara; Rocha, Welington Tristão da; Santos, Adelair Helena dos; Cravo, Pedro Vitor Lemos

doi:10.1590/0037-8682-0046-2015

Cited by 19 publications

(14 citation statements)

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“…14,15,[29][30][31][32][33][34] In the Americas, the co-occurrence of V1016I and F1534C has been found in the Caribbean Islands, Mexico, Brazil, Venezuela and the USA; in Africa, the co-occurrence of V1016I and F1534C in Ghana has been reported. 31,[35][36][37][38][39][40][41] In conclusion, we report here the co-occurrence of mutations V1016I and F1534C in populations of A. aegypti of the Colombian Caribbean region with variation in its allelic and genotypic frequencies. Although F1534C occurs more frequently in the populations studied compared to V1016I, the latter was correlated to resistance to pyrethroids in populations of A. aegypti of the Colombian Caribbean region.…”

Section: Discussionsupporting

confidence: 50%

Co‐occurrence of V1016I and F1534C mutations in the voltage‐gated sodium channel and resistance to pyrethroids in Aedes aegypti (L.) from the Colombian Caribbean region

Maestre‐Serrano

Pareja-Loaiza

Camargo

et al. 2019

Pest Management Science

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BACKGROUND Knockdown resistance is conferred primarily by non‐synonymous mutations that reduce pyrethroids binding to voltage‐gated sodium channels. In 2014, kdr mutation V1016I in Aedes aegypti populations resistant to pyrethroids was reported for the first time in Colombiα, in 2016 another kdr mutation, F1534C, and in 2018 the mutation V419L. Nine populations of A. aegypti, previously characterized as being resistant to λ‐cyhalothrin, deltamethrin, cyfluthrin and permethrin, were used for this study. Genomic DNA was used to determine genotypes by allele‐specific PCR for mutations V1016I and F1534C, and to determine their association with pyrethroid resistance. RESULTS All the populations analyzed showed both mutations, with allelic frequencies of 0.07–0.35 for I1016 and 0.47–0.88 for C1534. A percentage of co‐occurrence of mutant homozygotes I1016/C1534 of 5.3% was detected. A significant positive correlation was found between the frequency of the genotype I1016 and the resistance to permethrin, λ‐cyhalothrin and cyfluthrin, but not to deltamethrin; on the other hand, the correlation was not significant for the C1534 genotype and the four pyrethroids evaluated. No significant correlation was found between the frequencies of the mutations V1016I and F1534C. CONCLUSIONS Both mutations V1016I and F1534C are present in A. aegypti populations of the Colombian Caribbean, and although the frequency of F1534C exceeds V1016I, the latter was correlated to resistance to pyrethroid insecticides. © 2018 Society of Chemical Industry

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Section: Discussionsupporting

confidence: 50%

Co‐occurrence of V1016I and F1534C mutations in the voltage‐gated sodium channel and resistance to pyrethroids in Aedes aegypti (L.) from the Colombian Caribbean region

Maestre‐Serrano

Pareja-Loaiza

Camargo

et al. 2019

Pest Management Science

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“…However, the development of pyrethroid resistance is a major threat to the sustained use of pyrethroids in vector control. Historically, resistance has developed in Brazilian populations of disease-vector mosquitoes to a wide range of insecticides, including pyrethroids1622232425.…”

Section: Discussionmentioning

confidence: 99%

Detection of a new pyrethroid resistance mutation (V410L) in the sodium channel of Aedes aegypti: a potential challenge for mosquito control

Haddi

Tomé

et al. 2017

Sci Rep

174

217

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The yellow fever mosquito, Aedes aegypti, particularly in Neotropical regions, is the principal vector of dengue, yellow fever, Zika and Chikungunya viruses. Pyrethroids remain one of the most used insecticides to control Aedes mosquitoes, despite the development of pyrethroid resistance in many mosquito populations worldwide. Here, we report a Brazilian strain of A. aegypti with high levels (approximately 100–60,000 fold) of resistance to both type I and type II pyrethroids. We detected two mutations (V410L and F1534C) in the sodium channel from this resistant strain. This study is the first report of the V410L mutation in mosquitoes. Alone or in combination with the F1534C mutation, the V410L mutation drastically reduced the sensitivity of mosquito sodium channels expressed in Xenopus oocytes to both type I and type II pyrethroids. The V410L mutation presents a serious challenge for the control of A. aegypti and will compromise the use of pyrethroids for the control of A. aegypti in Brazil; therefore, early monitoring of the frequency of the V410L mutation will be a key resistance management strategy to preserve the effectiveness of pyrethroid insecticides.

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“…However, the V1016G mutation has not been reported in Latin America [31,32,46,47]. Instead, mutation V1016I was found, and often coexists with F1534C in South and North America, such as Venezuela [48], three French overseas territories [49], Brazil [50,51,52], Mexico [53], and the USA [54]. More recently, V1016I and F1534C were detected in Ghana, Africa [55], and a new mutation, T1520I, was found along with the F1534C mutation in a population in India [56].…”

Section: Identification Of Single Nucleotide Polymorphisms (Snps) mentioning

confidence: 99%

“…So far, V1016G has been detected only in Southeast Asia [34,37,40,41,44,45], whereas V1016I is distributed in South and North America [48,49,50,51,52,53]. More recently, V1016G, along with F1534C, was also detected in Ghana.…”

Section: Evolution Of Kdr Mutations In Aedes Aegyptimentioning

confidence: 99%

Sodium Channel Mutations and Pyrethroid Resistance in Aedes aegypti

Nomura

Zhorov

et al. 2016

Insects

134

142

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Pyrethroid insecticides are widely used to control insect pests and human disease vectors. Voltage-gated sodium channels are the primary targets of pyrethroid insecticides. Mutations in the sodium channel have been shown to be responsible for pyrethroid resistance, known as knockdown resistance (kdr), in various insects including mosquitoes. In Aedes aegypti mosquitoes, the principal urban vectors of dengue, zika, and yellow fever viruses, multiple single nucleotide polymorphisms in the sodium channel gene have been found in pyrethroid-resistant populations and some of them have been functionally confirmed to be responsible for kdr in an in vitro expression system, Xenopus oocytes. This mini-review aims to provide an update on the identification and functional characterization of pyrethroid resistance-associated sodium channel mutations from Aedes aegypti. The collection of kdr mutations not only helped us develop molecular markers for resistance monitoring, but also provided valuable information for computational molecular modeling of pyrethroid receptor sites on the sodium channel.

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Phenotypic and genotypic profile of pyrethroid resistance in populations of the mosquito Aedes aegypti from Goiânia, Central West Brazil

Cited by 19 publications

References 11 publications

Co‐occurrence of V1016I and F1534C mutations in the voltage‐gated sodium channel and resistance to pyrethroids in Aedes aegypti (L.) from the Colombian Caribbean region

Co‐occurrence of V1016I and F1534C mutations in the voltage‐gated sodium channel and resistance to pyrethroids in Aedes aegypti (L.) from the Colombian Caribbean region

Detection of a new pyrethroid resistance mutation (V410L) in the sodium channel of Aedes aegypti: a potential challenge for mosquito control

Sodium Channel Mutations and Pyrethroid Resistance in Aedes aegypti

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