Phenotypic and genotypic studies on antibiotic resistant Yersinia enterocolitica isolated from milk and milk products in Kaliobia, Egypt

Abdel‐Wahab, Ahmed; El-Tawab, Ashraf Awad Abd; Abdallah, Fatma Elzahraa; Maarouf, Ahmed Afifi

doi:10.21608/bvmj.2021.66845.1358

Cited by 5 publications

(4 citation statements)

References 21 publications

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“…Moreover, prevalence in Karish cheese (16%) was close to those obtained by Abd El Tawab et al [31] (14.3%), while this is higher than the results obtained by Rahimi et al [27] (11.7%) and [32] (6.7%). Also, the prevalence of Y. enterocolitica in cow milk (10%) was compatible to previous studies [27,32].…”

Section: Discussionsupporting

confidence: 80%

“…On the other hand, Pavlović et al [34] in Serbia and Zeinhom and Abdel-Latef [35] in Egypt performed poorly to isolate Y. enterocolitica from milk and milk products samples. There are likely a number of factors to blame for the discrepancies between this study's conclusions and those of several other writers, such as discrimination in sampling tactics for samples analyzed, methods of analysis, origins of samples, environment, humidity, health measures, season, and geographic position, which might have effect positive or negative transmission of Y. enterocolitica [31].…”

Section: Discussionmentioning

confidence: 59%

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Virulence Genotyping Profiles of Cefazolin Resistance Yersinia enterocolitica Isolated From Milk and Milk Products in Egypt

Dowidar

Khalifa

2023

Egyptian Journal of Veterinary Sciences

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T HE aim of this study was to investigate the incidence rate, characteristics, genes related with virulence, and determination of antibiotic resistance of pathogenic Yersinia (Y.) enterocolitica isolated from raw milk, Karish cheese, and ice cream. From July 2021 to May 2022, samples were obtained from popular markets, and street vendors in various places in Mansoura city, Dakahlia, Egypt. Milk, Karish cheese, and ice cream (50 samples each) were positive in 26 of 150 samples (17.3%) for Yersinia enterocolitica, with a higher prevalence in ice cream (26%). Among the 26 Y. enterocolitica isolates, three virulence genes (ail, inv, and yadA) had different frequency profiles. The inv gene was found in a significant majority of the strains tested (73.1%), followed by the ail gene (50%) and the yadA gene (26.9%). Gentamicin and ciprofloxacin were estimated to have a high sensitivity rate ((96.2% each), followed by sulphamethoxazol-trimethoprim and chloramphenicol (92.3%), then cephalotin and kanamycin (84.6%), finally fosfomycin (73.1%). Interestingly, 53.8% of strains were identified as the multidrug resistance, with 11 different resistance patterns. Nineteen strains of Y. enterocolitica showed biofilm-forming ability, of which seven strains had the high biofilm-forming capacity and eight other strains of Y. enterocolitica had moderate biofilmforming capacity. The discovery of presumably pathogenic Y. enterocolitica isolates from milk and milk products implies a hazard to consumer safety especially due to the occurrence of pathogenicity potential and antibiotic resistance which necessity the implementation of control measures.

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Section: Discussionsupporting

confidence: 80%

Section: Discussionmentioning

confidence: 59%

Virulence Genotyping Profiles of Cefazolin Resistance Yersinia enterocolitica Isolated From Milk and Milk Products in Egypt

Dowidar

Khalifa

2023

Egyptian Journal of Veterinary Sciences

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“…Moreover, they were highly sensitive to ampicillin. While Abdelwahab et al . (2021 ) reported that Y. enterocolitica was highly sensitive to norfloxacin and meropenem (79.0% for each).…”

Section: Discussionmentioning

confidence: 99%

Inhibitory effect of some probiotic strains and essential oils on the growth of some foodborne pathogens

Fathy,

Awad,

Aal

et al. 2024

Open Vet J

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Background: Bacillus cereus and Yersinia enterocolitica are implicated in foodborne diseases (FBD) which have major effects on human health, therefore it is considered as universal public-health disorders. Essential oils and essential oils Nano emulsions have a sufficient antibacterial performance against a variety of bacteria especially multi-drug resistant bacteria. Probiotics showed several health benefits via moderating the GIT microbiota and their metabolites. Aim: The study was designed for evaluating the biocontrol ability of cinnamon essential oil Nano emulsion and probiotics as natural antibacterial additives and reveal their bactericidal mechanism. Methods: 250 random samples (50 raw milk, 50 rice pudding, 50 kariesh cheese, 50 yoghurt and 50 ice cream) were purchased separately from different areas in Mansoura city, Egypt and exposed to bacteriological analysis. Results: Bacillus cereus was found with the highest mean value of 66x107 ± 1.3x108 CFU/g in raw milk and the lowest mean value of 28x107 ± 2.6x107 CFU/g in kariesh cheese while Yersinia enterocolitica were found in 64% of the total inspected samples with the highest incidence (84%) in yoghurt. The toxinogenic potential of the tested pathogens have been evaluated by multiplex PCR pointing nhe A and ces genes for Bacillus cereus isolates while targeting in Y. enterocolitica 16s rRNA, and YST gene. Different concentrations (0.17%, 0.25%, 0.5%, 0.8%, 1%, 1.5%, and 2%) of cinnamon oil Nano emulsion were employed in this study. CEO (Cinnamon essential oil) Nano emulsion had the highest reduction rate at a concentration of 1.5% in case of Bacillus cereus and 2% in case of Y. enterocolitica. Among different types of probiotics, the best one which showed inhibitory potential against Bacillus cereus and Y. enterocolitica was L. plantarum. Conclusion: L. plantarum and CEO Nano emulsion at a concentration of 2% have the highest reduction rate against Y. enterocolitica while, L. plantarum and CEO Nano emulsion at a concentration of 1.5% have the best antibacterial effect against Bacillus cereus. In conclusion, more attention is required for both safety and quality in dairy products through application of natural additives as essential oils and probiotics.

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“…In Egypt, identification, and molecular characterization of Y. enterocolitica has been carried out in meat, milk, and their products [17][18][19]. However, little is known about the prevalence of such infection in dogs, cats, and their owners.…”

Section: Introductionmentioning

confidence: 99%

Prevalence and Molecular Characterization of Yersinia species Isolated from Dogs and Cats

Nasser

Abdou

Gwida

et al. 2023

Egyptian Journal of Veterinary Sciences

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Y ersinia infection is one of the five main bacterial gastrointestinal diseases of humans.Limited information on the presence of Yersinia spp. from companion animals, especially dogs and cats were found. Therefore, this study was carried out to investigate the existence of Yersinia spp. in companion animals and to evaluate the antimicrobial profile of zoonotic isolates using classical microbiological techniques and multiplex PCR targeting four virulenceassociated genes ystA (Yersinia stable toxin gene), ail (attachment-invasion locus gene), inv (invasion gene) and yadA (Yersinia adhesion gene). The study included 200 fecal samples from (cats n= 120 and dogs n= 80), and stool specimens (n=50) from contact owners. Twenty-nine (14.5%) out of 200 fecal samples were found to be positive for Yersinia species which were identified as Y. enterocolitica was (51.7%), Y. intermedia (20.6%), moreover, Y. Frederiksen (27.5%). For human stool specimens, Yersinia species found to 8 (16%), and Y. enterocolitica 50 %. The sensitivity rates of Y. enterocolotica to the following antibiotics ciprofloxacin, cefepime, imipenem, norfloxacin, amikacin, cefotaxime was 94.7, 84.2%, 78.4%, 78.4%, 68.4% and 57.9%, respectively. In conclusion, Yersinia species particularly Y. enterocolitica and related species are existing in dogs, cats, and their human contacts in our locality. Recovery of such enteropathogens and identification of the virulent genes provide an information about the public health hazard and zoonotic role played by such pets as a source and reservoirs. Identification of prevalence of such infection may help to construct the ideal treatment and preventive measures.

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Phenotypic and genotypic studies on antibiotic resistant Yersinia enterocolitica isolated from milk and milk products in Kaliobia, Egypt

Cited by 5 publications

References 21 publications

Virulence Genotyping Profiles of Cefazolin Resistance Yersinia enterocolitica Isolated From Milk and Milk Products in Egypt

Virulence Genotyping Profiles of Cefazolin Resistance Yersinia enterocolitica Isolated From Milk and Milk Products in Egypt

Inhibitory effect of some probiotic strains and essential oils on the growth of some foodborne pathogens

Prevalence and Molecular Characterization of Yersinia species Isolated from Dogs and Cats

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