Phenotypic and plant growth promoting characteristics of Rhizobium leguminosarum bv. viciae from lentil growing areas of Ethiopia

Jida, Mulissa

doi:10.5897/ajmr11.400

Cited by 15 publications

(4 citation statements)

References 30 publications

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“…All 22 bacterial isolates showed circular colonies of bacilli, cocci, and coccobacillus type. The pH of the culture medium, as indicated by BTB, became acidic for fast growing isolates within 24 to 48 hours after incubation and the culture medium became yellow, as described by [41].…”

Section: Resultsmentioning

confidence: 97%

Isolation, Characterization, and Selection of Bacterial Endophytes from Soybean (Glycine max) Nodules and Roots in Some Soils of Cameroon for Promoting Growth of Forage Legume Plants

Bahdjolbe,

Sontsa-Donhoung,

Abdouraman

et al. 2023

MRJI

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Aim: The present study aimed to isolate, characterize and select the most effective bacterial endophytes to enhance soybean growth, biomass, and yield. Methodology: Seven soil samples were collected from the rhizosphere of forage legumes in fields across three agroecological zones of Cameroon. Bacterial endophytes were isolated from soybean roots and nodules and cultured on nutrient agar. The isolates were screened for the tolerance tests, extracellular enzymatic activity, P-solubilization activity, and IAA production. The best isolates were selected using a two-factor block factorial design. Each treatment was replicated six times. Results: A total of 85 bacterial endophytes were isolated. Characterization results of 22 preselected isolates revealed an optimal growth temperature of 37°C and a pH range between 6 and 7. Furthermore, the 22 isolates solubilized inorganic phosphate, 7 produced IAA, and 8 exhibited amylase activity. PCR analysis of the nifH and nodC genes showed that the isolates possessed the nifH gene as a nitrogen fixation marker and the nodC gene as a nodulation marker. The findings show that, out of the 22 bacterial endophyte isolates, NTT1 and BOSH9 were the most effective in increasing plant height by 26.74% and 31.78%, respectively. Additionally, they resulted in an increase in biomass of 94.24% to 120.48% and an increase of 71.59% to 76.70% in grain yield compared to control treatment. Conclusion: The selected isolates significantly enhance plant growth, increase biomass, and improve soybean grain yield. However, their potential use as biofertilizers in agriculture will require further investigation under real field conditions.

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Section: Resultsmentioning

confidence: 97%

Isolation, Characterization, and Selection of Bacterial Endophytes from Soybean (Glycine max) Nodules and Roots in Some Soils of Cameroon for Promoting Growth of Forage Legume Plants

Bahdjolbe,

Sontsa-Donhoung,

Abdouraman

et al. 2023

MRJI

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“…Amino acids utilization test is one of the necessary physiological characteristics for the selection of isolates for different ecological conditions (Jida & Asefa, 2011). This study revealed that there is a difference among cowpea nodulating isolates in utilizing different nitrogen sources.…”

Section: Amino Acid Utilizationmentioning

confidence: 91%

“…These isolates have been known to utilize a broader range of nitrogen sources making them predominantly significant for the production of inoculum for soils with different nitrogen sources. Jida and Asefa (2011) indicated that the capability of isolates to use a broader range of nitrogen sources gave them survival and competitive advantage in the soil.…”

Section: Amino Acid Utilizationmentioning

confidence: 99%

Eco-physiological and physiological characterization of cowpea nodulating native rhizobia isolated from major production areas of Ethiopia

et al. 2020

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“…BTB is used as pH indicator, the coloration is used to distinguish between slow growers -Bradyrhizobium (alkaline reaction-blue) and fast growers -Rhizobium strains (acidic reactionyellow). For testing the purity of Rhizobium cultures, Congo red dye is often added to culture medium [15].…”

Section: Morphology Characterizationmentioning

confidence: 99%

Isolation and Characterization of Plant Growth Promoting Bacteria from Root Nodules of Cicer arientinum L.

Chandra

Chakraborty

2023

IJPSS

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Aims: The aim of the present study is to isolate and characterize root nodulating bacteria from Cicer arientinum L cv. GNG 1958 and assess its plant growth promoting properties. The study is based on previous reports that native symbiotic bacteria may be replaced by better performing strains by corss-inoculation to increase productivity and tolarance to Biotic and abiotic stresses. Study Design: Isolation of symbiotic bacteria, its biochemical and molecular characterization and assessment of plant growth promoting property through ammonia production, phosphate solubilization and HCN production. Place and Duration of Study: Department of Bioscience and Biotechnology, Banasthali Vidyapith, Rajasthan, during 2019-2022. Methodology: Root nodulating bacterial strain was isolated from C. arientinum L. cv. GNG 1958 grown at the agricultural fields' located at the Krishi Vigyan Kendra, Banasthali Vidypapith, Rajasthan. Morphological characteristics of the isolates were noted following Bergey’s Manual of Systemic Bacteriology and biochemical tests performed as per standard methods. Molecular characterization of the potential symbiont was performed by PCR of 16s rRNA genes and the PCR product was sequenced. Plant growth promoting property was determined through ammonia production, phosphate solubilization and HCN production. Results: Root nodulating bacterial strain was isolated from C. arientinum L.cv. GNG 1958. Out of eighteen isolates, BVCA-58 grew fast and changed the color of BTB dye in media from green to yellow and deep yellow within 2 days of incubation. White colonies were observed when BVCA-58 was inoculated on YEMA media supplemented with Congo red. BVCA-58 has the ability to produce hydrogen cyanide, ammonia and solubilize phosphate. Molecular study of BVCA-58 using 16f-16r and 63f-1244r primers showed 99.89% and 99.62% of similarity with Mesorhizobium jarvisii ATCC-33669 and Mesorhizobium erdmanii USDA-3471 respectively on performing NCBI Blast. BVCA-58 was submitted to NCBI portal as Mesorhizobium sp. strain BVCA-58 with accession nos. OP646813 and OP646810. Conclusion: The present study revealed that isolated strain from Cicer arientinum GNG 1958 variety was Mesorhizobium sp. which has potent plant growth promoting traits.

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Phenotypic and plant growth promoting characteristics of Rhizobium leguminosarum bv. viciae from lentil growing areas of Ethiopia

Cited by 15 publications

References 30 publications

Isolation, Characterization, and Selection of Bacterial Endophytes from Soybean (Glycine max) Nodules and Roots in Some Soils of Cameroon for Promoting Growth of Forage Legume Plants

Isolation, Characterization, and Selection of Bacterial Endophytes from Soybean (Glycine max) Nodules and Roots in Some Soils of Cameroon for Promoting Growth of Forage Legume Plants

Eco-physiological and physiological characterization of cowpea nodulating native rhizobia isolated from major production areas of Ethiopia

Isolation and Characterization of Plant Growth Promoting Bacteria from Root Nodules of Cicer arientinum L.

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