Phenotypic, Biochemical and Genotypic Charcaterisation of Some Factors Involved in the Virulence and Survival of Bacteria Isolated from Food and Food Processing Surfaces

Self Cite

Antibiotherapy is the main determinant of Clostridium difficile infection due to the imbalance determined in the intestinal flora. Clostridium difficile infection can be considered a current public health problem, given the increased incidence, both as a nosocomial infection as well as at community level, by excessive, uncontrolled and unjustified use of antibiotics, high contagiousness, negative influence on health systems in the increased number of days of hospitalization and implicitly increased costs, and last but not least, the substantial deterioration of the quality of the patient�s life. The retrospective study over a 12-month period over a group of 106 patients revealed the following profile of the patient affected by Clostridium difficile infection: females, aged 66.75 � 15.66, from the urban area, hospitalized in the medical section, who had diarrhea more than 2 days after admission, but up to 28 days after this event, due to prolonged antibiotic therapy with ceftriaxone, associated with a gastric secretion inhibitor, on a background of multiple associated pathologies. It is very important for all health systems to control this phenomenon and this is only possible by applying effective measures to prevent the onset of CDI, of relapses and contamination, thus identifying the judicious use of antibiotics.

Section: Resultsmentioning

confidence: 99%

Corelations Between the Use of Medical Substances and the Incidence Infections Produced by Clostridium Difficile Species

Tantu¹,

Man²,

Păunescu³

et al. 2018

Self Cite

“…Uncomplicated abscesses are routinely treated by incision and drainage alone, but antibiotics such as tetracyclines, trimethoprim/sulfamethoxazole and clindamycin can also be added to the therapy [30]. Linezolid and glycopeptides are used in severe, hospital-acquired infections [8,30,40].…”

Section: Figure 6 Distribution Of Antimicrobial Resistance Accordingmentioning

confidence: 99%

The Need to Develop New Antimicrobial Molecules, as Revealed by in vitro Assessment of Drug Resistance in Staphylococcal Skin Infections Treated with Autologous Bacterial Vaccine

Preda¹,

Serbanescu²,

Mihai³

et al. 2020

Staphylococcus spp. is a facultative pathogen, which can be found in the commensal microbiota of humans, most often in moist skinfolds and mucous membranes. This microorganism has the ability to cause various infections, in almost every organ of the body, with an increased frequency in the skin and soft tissues, being involved in pathologies like acne, folliculitis, furunculosis, hidradenitis suppurativa, cellulitis, abscesses, but also in secondary infections in diseases with an altered cutaneous barrier. The prolonged evolution of these diseases and severe outcome can be influenced by various factors, most importantly being the antimicrobial resistance. We have evaluated the antimicrobial susceptibility profiles, according to the Comite de l` Antibiogramme de la Societe Francaise de Microbiologie recommendations, for strains of Staphylococcus spp. isolated from acne or different types of skin and soft tissue infections in patients recommended to receive autologous bacterial vaccine. Most frequent identified species was Staphylococcus epidermidis, followed by Staphylococcus aureus. The antimicrobial resistance was higher for antibiotics usually used in the treatment of skin and soft tissue infections, with interesting differences of the resistance profile for the strains isolated from patients before receiving autologous bacterial vaccine compared with the ones from individuals already treated. Another important finding was represented by the differences in the resistance profile according to the age group of the patients. The results of this study underline the importance of antimicrobial resistance surveillance in finding new molecules and alternative therapies, the necessity of a personalized approach in medical acts and of a continuous connection between clinic and laboratory research.

“…Groundwater represents an important source of drinking water and most of health problems related to water consumption have been linked to microbial contamination [1,2], but serious health problems can also arise due to the chemical pollution as a result of recent or past human activities [3,4].…”

mentioning

confidence: 99%

Antibiotic Resistance Profile and Chemical Quality Assessment of Groundwater Sources from Periurban Area of Bucharest, Romania

Marinescu¹,

Ilie²,

Ghita³

et al. 2019

Twenty-two groundwater sources mainly used for drinking purpose in Bucharest peri-urban area were investigated for assessment of physico-chemical and microbiological quality with a view to determining its potential risk to public health. Results of chemical analysis revealed that nitrites, sulphates and chlorides were below the permissible levels, while 63.64% of the analysed groundwater sources exceeded the maximum admissible concentration for nitrates, with concentration variations ranging from 67.27 to 523.19 mg/L. The bacteriological analysis showed that in about 63% of groundwater sources total coliform, faecal coliform and enterococci have exceeded the threshold limits recommended by the Drinking Water Directive 98/83/EC and the Romanian Law. Another aim of the study was to investigate the prevalence of antibiotic resistance among Gram-negative strains isolated from groundwater sources. There observed the resistance to many antibiotics, particularly: ticarcillin (80%), aztreonam (29%), gentamicin (11%), imipenem (9%), ceftriaxone (9%), ceftazidime (3%) and ciprofloxacin (3%). Significant higher resistance rates were observed in strains isolated from shallow groundwater sources as compared with strains isolated from deep groundwater sources. Pseudomonas sp. (26%) isolates with multiple-drug resistance (MDR) were encountered. The results of the study revealed a bacteriological contamination and high levels of nitrate concentrations in most of the groundwater samples, which could pose an important risk to human health.