Phenotypic characterisation of Candida albicans isolated from chronic hyperplastic candidosis

Williams, David Wynne; Wilson, Matthew; Potts, A. J. C.; Lewis, Michael Alexander Oxenham

doi:10.1099/0022-1317-49-2-199

Cited by 7 publications

(7 citation statements)

References 18 publications

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“…Among six of the C. albicans strains isolated from H. verbana, two biochemical phenotypes (2576154, 2576174 -API-20C AUX) were predominant and they were detected both from cultures of jaws/pharynx and body surface, as well as from water samples. These same Candida strains, with numerical assimilation profiles 2576174 and 2576154, were found most frequently by other authors in both people with fungal skin colonisation and candidosis (Williams et al, 2000;Glowacka, 2002). In particular, a strain of C. albicans coded 2576174 is the most common strain observed in people with symptomatic candidoses (Williams et al, 2000).…”

Section: Discussionsupporting

confidence: 65%

Hirudo verbana is a source of fungal isolates potentially pathogenic to humans

Litwinowicz¹,

Błaszkowska²

2013

Afr. J. Microbiol. Res.

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As microbial contamination of leeches poses a risk of transmission of pathogens to humans, contact with non-sterile leeches causes a potential hazard to human health. The purpose of this study was to evaluate the mycological purity of the body surface, jaws/pharynx and intestines of the medical leech, Hirudo verbana, and the purity of aquarium water within which the leeches were incubated. Leeches were kept without feeding under optimum laboratory conditions recommended for medicinal uses. The strains of fungi were isolated according to our own methods and standard mycological procedures. Of the 150 cultures taken from 50 leeches and 50 samples of water, 152 strains of yeast-like fungi and yeasts belonging to 14 species and 3 genera were identified. The greatest number of fungal species (11) was isolated from the leech jaws, next (10) from the body surface, while the fewest species (8) were found from the samples of water in which the animals were maintained. Fungal isolates belonging to biosafety level two (BSL-2), classified as potential pathogens for humans identified were Candida albicans, Candida ciferrii, Candida krusei, Candida tropicalis, Trichosporon asahii and Trichosporon asteroides. Some other isolates with a decreased pathogenicity potential (BSL-1) also identified were Candida guilliermondii, Candida famata, Candida lambica, Candida parapsilosis and Rhodotorula rubra. The isolation of a high number of yeast-like fungal strains from H. verbana suggests that this medical leech is a vector of potentially pathogenic human fungal species.

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Section: Discussionsupporting

confidence: 65%

Hirudo verbana is a source of fungal isolates potentially pathogenic to humans

Litwinowicz¹,

Błaszkowska²

2013

Afr. J. Microbiol. Res.

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“…In Sweden, Torssander et al (1996) observed that, among homosexual men, the prevalence of serotype B was 40% in HIV-infected patients and 39% in HIV-seronegative patients, while in control subjects of heterosexual non-HIV men, the prevalence was only 7.5%. In Europe, nevertheless, HIV-seronegative populations seem to have also a greater prevalence of serotype A (McMullan-Vogel et al 1999, Williams et al 2000.…”

Section: Discussionmentioning

confidence: 99%

Multicenter Brazilian Study of Oral Candida Species Isolated from Aids Patients

Santana

Milan

Martínez

et al. 2002

Mem. Inst. Oswaldo Cruz

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“…The study was performed to help clarify the conflicting reports of an association of particular C. albicans biotypes with CHC (25,43). Three PCR fingerprinting methods involving IR PCR (primers 1245 and 1246 and primer 1251) and M13 PCR-mediated genotyping were used to assess the genetic relatedness of C. albicans isolates from CHC and non-CHC patient populations.…”

Section: Discussionmentioning

confidence: 99%

“…Examples include biotype differences between isolates from CHC and normal mucosa and phenotypic variation between isolates recovered from speckled and homogeneous forms of CHC (10,25). However, a recent biotyping investigation performed at Cardiff Dental School failed to identify such clonal restriction of 35 C. albicans isolates from CHC (43). The main problem of biotyping C. albicans is that a large number of epidemiologically unrelated strains frequently have identical biotypes, with a single profile predominating for the majority of isolates (36).…”

mentioning

confidence: 99%

PCR Fingerprinting of Candida albicans Associated with Chronic Hyperplastic Candidosis and Other Oral Conditions

et al. 2001

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The purpose of this study was to genotype strains of Candida albicans to determine whether specific types were associated with chronic hyperplastic candidosis (CHC). A total of 67 candidal isolates from CHC patients (n ‫؍‬ 17) and from patients with other oral conditions (n ‫؍‬ 21) were genotyped by PCR fingerprinting employing two interrepeat primer combinations (1245 and 1246 primers or 1251 primer) and a single minisatellite-specific M13 primer. The most suitable primer for fingerprint analysis was found to be primer 1251, yielding well-resolved banding patterns. For the 67 isolates tested, PCR fingerprinting delineated 25 (1245 and 1246 primers), 27 (1251 primer), and 25 (M13 primer) profiles. The majority of C. albicans isolates from multiple sites within the mouth produced identical profiles (six out of nine subjects examined). For patients for whom a series of longitudinal isolates was available, strain persistence for up to 7 years was evident for five out of eight individuals, despite episodes of antifungal therapy. Computer-assisted comparison of the interrepeat PCR fingerprints identified seven distinct profiles that were shared among isolates from different individuals. However, no association was evident among isolates of C. albicans from specific clinical conditions. Eight isolates that were initially identified as C. albicans but having atypical PCR profiles were later confirmed as Candida dubliniensis. In conclusion, the genotypic data do not indicate clonal restriction of C. albicans with respect to CHC. Furthermore, these results have demonstrated that in the majority of individuals, colonizing populations of C. albicans are clonal in nature and exhibit strain persistence.

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Phenotypic characterisation of Candida albicans isolated from chronic hyperplastic candidosis

Cited by 7 publications

References 18 publications

Hirudo verbana is a source of fungal isolates potentially pathogenic to humans

Hirudo verbana is a source of fungal isolates potentially pathogenic to humans

Multicenter Brazilian Study of Oral Candida Species Isolated from Aids Patients

PCR Fingerprinting of Candida albicans Associated with Chronic Hyperplastic Candidosis and Other Oral Conditions

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