Phenotypic characteristics of tumour-infiltrating lymphocytes in human oesophageal cancer tissues defined by quantitative two-colour analysis with flow-cytometry

Tahara, Hideaki; Shiozaki, Hitoshi; Kobayashi, Kenji; Yano, Tokiharu; Yano, Hiroshi; Tamura, Shigeyuki; Oku, Kunihiko; Miyata, Masashi; Wakasa, Kenichi; Sakurai, Masami; Mori, Takesada

doi:10.1007/bf01605293

Cited by 10 publications

(4 citation statements)

References 21 publications

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“…differences between tumorous and grossly nor mal tissues adjacent to a tumor after treatment with DMH were not so marked as those observed following treatment with MNNG. Histologically, tumorous tissues obtained after all types of treatment were characterized by a sharp decrease in the amount of goblet cells and by an increase in the amount of lymphocytes infiltrating between the crypts and penetrated in their lumen that conforms with observa tions in the literature [18].…”

Section: Discussionsupporting

confidence: 56%

Morphological, Histochemical and Immunohistochemical Differences between Tumorous and Adjacent Tissues in Chemically Induced Colon Cancer in Rats

Zusman¹,

Zimber²,

Madar

et al. 1992

Cells Tissues Organs

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Methods of morphological, histochemical and immunohistochemical analyses were used to further characterize differences between tumorous and adjacent grossly normal tissues in chemically-induced colon cancer in rats. Colon tumors were induced by the treatment of rats with 1,2-dimethylhydrazine or with N-methyl-N’-nitro-N-nitrosoguanidine alone or with subsequent treatment with deoxycholic bile acid. Tissues were studied morphologically (for the presence of goblet cells in the colon crypts, and the extent of infiltration of lymphocytes into the crypts and between them) histochemically (for the presence of positive reaction to neutral and acid mucopolysaccharides) and immunohistochemically (for the presence of tissue polypeptide antigen). All data were evaluated quantitatively, and index of tissue damage was calculated for both tumorous and non-tumorous tissues. Significant morphological differences were found between tumorous and adjacent apparently normal tissue. Histochemically and immunohistochemically, both types of tissue reacted very similarly to exposure to the carcinogens. Index of damage was significantly different from normal untreated colon in both kinds of tissue. It was suggested that precancerous state in tissue adjacent-to-tumor could be detected using the combination of these methods.

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Section: Discussionsupporting

confidence: 56%

Morphological, Histochemical and Immunohistochemical Differences between Tumorous and Adjacent Tissues in Chemically Induced Colon Cancer in Rats

Zusman¹,

Zimber²,

Madar

et al. 1992

Cells Tissues Organs

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“…Indeed, cytotoxic, memory CD8+ T‐cell infiltrates within tumors are strongly correlated with reduced disease recurrence and prolonged survival following various therapeutic settings of malignant melanoma, colorectal carcinoma, esophageal carcinoma, liver cancer, and renal carcinoma. ( 3–10 ) Together, these findings suggest a potential contribution for nascent host immune responses in modulating the clinical outcome of human malignant diseases.…”

Section: Cytokines Function As “Double‐edged Sword” Against Cancersmentioning

confidence: 86%

Cytokine gene‐mediated immunotherapy: Current status and future perspectives

Jinushi¹,

Tahara²

2009

Cancer Science

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“…Both of these parameters are characterized by a high range of variability between different rats ( Table 2). In humans, lymphatic penetration into the submucosal layer is a very characteristic parameter of malignant growth in the colon (Tahara et al, 1990;Wilson & Donohue, 1991).…”

Section: Zusmanmentioning

confidence: 99%

Variability of Neoplastic Parameters in Colon Tumours, and Its Significance in Diagnostic Practice

Zusman

1995

Biological Reviews

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We have reviewed the value of individual variability in the reaction of tissues to treatment with carcinogens, and the manifestation of this variability in different morphological (histological, morphometric, and ultrastructural), histochemical and immunohistochemical parameters generated in tumorous tissues. Moreover, we also reviewed data in the literature on individual variability in the manifestation of some biochemical and immunochemical markers which are accumulated in the serum of tumour-bearing patients and which are characteristic for the different phases of tumourigenesis. The high variability of different tumorous parameters suggests that none can be utilized alone as a conclusive marker of neoplasia and that only their combined use can give objective information. We also reviewed the impact of this variability in the evaluation of various pathological methods to detect different stages of neoplastic transformation in the colon. It has been concluded that none of the present approaches can be absolutely conclusive and without false results, and that objective information regarding early cancerous changes may be obtained only by use of combined analyses utilizing several laboratory methods. The diagnostic procedures should be based on the complex utilization of all appropriate methods using the quantitative interpretation of the obtained data. Multivariate analysis of many parameters should be very effective for the prediction of therapeutic results.

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Phenotypic characteristics of tumour-infiltrating lymphocytes in human oesophageal cancer tissues defined by quantitative two-colour analysis with flow-cytometry

Cited by 10 publications

References 21 publications

Morphological, Histochemical and Immunohistochemical Differences between Tumorous and Adjacent Tissues in Chemically Induced Colon Cancer in Rats

Morphological, Histochemical and Immunohistochemical Differences between Tumorous and Adjacent Tissues in Chemically Induced Colon Cancer in Rats

Cytokine gene‐mediated immunotherapy: Current status and future perspectives

Variability of Neoplastic Parameters in Colon Tumours, and Its Significance in Diagnostic Practice

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