Phenotypic Characterization and Random Amplified Polymorphic DNA (RAPD) analysis of &lt;i&gt;Pasteurella multocida&lt;/i&gt; isolated from Korean Pigs

Lee, Ki-Eun; Jeoung, Hye-Young; Lee, Jiyoun; Lee, Myoung-Heon; Choi, Hwan-Won; Chang, Kyung-Soo; Oh, Youjin; An, Dong-Jun

doi:10.1292/jvms.11-0418

Cited by 11 publications

(17 citation statements)

References 14 publications

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“…In South Korea, all isolates of P. multocida serogroup D were in the same cluster and showed 100% of similarity (LEE et al, 2012).…”

Section: Resultsmentioning

confidence: 92%

“…TAYLOR et al (2010) used the same primer (M13) to characterize 41 isolates of P. multocida isolated from cattle with respiratory diseases and found 13 clusters in which each isolate generated DNA fragment patterns containing 12 to 18 bands. In another study, LEE et al (2012) characterized 47 isolates of P. multocida serogroup A from swine by RAPD with the same primer and found 12 clusters. These studies demonstrate that the M13 primer used for RAPD is useful for genetic differentiation of the isolates.…”

Section: Resultsmentioning

confidence: 99%

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Molecular characterization of Pasteurella multocida isolates from swine lungs by Randomly Amplified Polymorphic DNA

et al. 2015

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“…In South Korea, all isolates of P. multocida serogroup D were in the same cluster and showed 100% of similarity (LEE et al, 2012).…”

Section: Resultsmentioning

confidence: 92%

Section: Resultsmentioning

confidence: 99%

Molecular characterization of Pasteurella multocida isolates from swine lungs by Randomly Amplified Polymorphic DNA

et al. 2015

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“…P. multocida prevalence has been reported as 8.0% in diseased pigs with pneumonia or PAR in China, and from 10.3 to 15.6% in pigs with pneumonia in Korea. Additionally, P. multocida constitutes 15.6% of isolated respiratory pathogens in the United States [3, 5, 7, 8].…”

Section: Introductionmentioning

confidence: 99%

Characterisation of Pasteurella multocida isolates from pigs with pneumonia in Korea

Kim

et al. 2019

BMC Vet Res

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Background Pasteurella multocida is responsible for significant economic losses in pigs worldwide. In clinically diseased pigs, most P. multocida isolates are characterised as subspecies multocida , biovar 2 or 3 and capsular type A or D; however, there is little information regarding subspecies, biovars, and other capsular types of P. multocida isolates in Korea. Here, we provided information covering an extended time period regarding P. multocida in pigs with pneumonia in Korea using phenotypic and genotypic characterisations and data associated with the minimum inhibitory concentrations. Results The overall prevalence of P. multocida between 2008 and 2016 was 16.8% (240/1430), with 85% of the P. multocida isolates (204/240) coinfected with other respiratory pathogens. Of the 240 isolates, 166 were included in this study; all of these P. multocida isolates were characterised as subspecies multocida and the most prevalent phenotypes were represented by biovar 3 (68.7%; n = 114) and capsular type A (69.9%; n = 116). Additionally, three capsular type F isolates were identified, with this representing the first report of such isolates in Korea. All biovar 1 and 2 isolates were capsular types F and A, respectively. The virulence-associated gene distribution was variable; all capsular type A and D isolates harboured pmHAS and hsf-1 , respectively ( P < 0.001), with type F (biovar 1) significantly correlated with hsf-1 ( P < 0.05) and pfhA ( P < 0.01), biovar 2 highly associated with pfhA and pmHAS , and biovar 3 significantly correlated with hsf-1 , pmHAS , and hgbB ( P < 0.001), whereas biovar 13 was related only to hgbB ( P < 0.05). The highest resistance rate was found to be to oxytetracycline (63.3%), followed by florfenicol (16.3%). Conclusions P. multocida subspecies multocida , biovar 3, and capsular type A was the most prevalent isolate in this study, and our findings indicated the emergence of capsular type F in Korea. Moreover, prudent use of oxytetracycline and florfenicol is required because of the identified high resistance rates. Further studies are required for continuous monitoring of the antimicrobial resistance, prevalence, and epidemiological characterisation of P. multocida , a...

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“…Along with Mycoplasma species, P multocida is one of the most common pathogens causing single or complex respiratory disease in Korean pigs. 3 5 However, limited information is available regarding the antimicrobial susceptibility trends of recently isolated P multocida strains. Therefore, the purpose of this study was to evaluate changes occurring over a period of seven years (2010–2016) in the resistance of P multocida isolates to 18 antimicrobials routinely used to treat pigs.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial resistance ofPasteurella multocidastrains isolated from pigs between 2010 and 2016

Moon

Lee

et al. 2018

Veterinary Record Open

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Pasteurella multocida is one of the significant causes of respiratory infection outbreaks in the Korean pig industry. Although antimicrobial treatment is an effective strategy for controlling respiratory diseases, limited information is available regarding the antimicrobial susceptibility of the pathogens infecting Korean pigs. Therefore, in this study, we evaluated the antimicrobial resistance of P multocida against widely used antimicrobials in order to enable the selection of appropriate drugs and to evaluate any trends in resistance. A total of 454 isolates of P multocida were collected from all provinces in Korea between 2010 and 2016. Antimicrobial susceptibility of all isolates was determined using a broth microdilution method. The most frequently observed resistance was to sulphadimethoxine (76.0 per cent), followed by oxytetracycline (66.5 per cent), chlortetracycline (36.8 per cent) and florfenicol (18.5 per cent). Although no consistent increase or decrease in resistance was observed for most antimicrobials, resistance to fluoroquinolones tended to increase over the study period. A variety of resistance patterns were observed, most frequently for tetracyclines and sulphonamides. These findings could provide information enabling the selection of optimal antimicrobials for efficient treatment of pneumoniae pasteurellosis in pig farms, which would impede the emergence of antimicrobial resistance.

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Phenotypic Characterization and Random Amplified Polymorphic DNA (RAPD) analysis of <i>Pasteurella multocida</i> isolated from Korean Pigs

Cited by 11 publications

References 14 publications

Molecular characterization of Pasteurella multocida isolates from swine lungs by Randomly Amplified Polymorphic DNA

Molecular characterization of Pasteurella multocida isolates from swine lungs by Randomly Amplified Polymorphic DNA

Characterisation of Pasteurella multocida isolates from pigs with pneumonia in Korea

Antimicrobial resistance ofPasteurella multocidastrains isolated from pigs between 2010 and 2016

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