Phenotypic characterization of extended-spectrum-beta‑lactamase producing E. coli from healthy individuals, patients, sewage sludge, cattle, chickens and raw meat

Saleem, Rabia; Ejaz, Hasan; Zafar, Ali; Younas, Sonia; Rathore, Ahsan Waheed

doi:10.12669/pjms.334.12647

Cited by 13 publications

(13 citation statements)

References 16 publications

(23 reference statements)

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“…Thus, comparison of our results from different countries is may not be feasible due to differences in the study methodology, such as method of enrichment and isolation procedures, differences in sample size and type of sample and how and when it was collected. However, the proportion of ESBL- producing E. coli strains obtained in this study was lower than the study finding in Vietnam [14] and Pakistan [15] and higher than the study finding in China [16]. In addition to the above methodological differences, the observed differences might be also related with the frequent and miss-use practices of third generation cephalosporins in humans and food animal, since the use of these antibiotics greatly linked to the recent emergence of ESBLs-producing bacteria.…”

Section: Discussioncontrasting

confidence: 90%

“…In this study 26 (74.3%) of E. coli isolates were showed co-resistance to three or more classes of antibiotics, such as tetracycline, co-trimoxazole and erythromycin plus β- lactam group of antibiotics. The higher rate of multi-drug resistant (MDR) isolates were also reported in other area of Ethiopia [32], Vietnam [14] and Pakistan [15], in which (56.5 to 96.3%) of ESBLs-producing and non- ESBLs-producing E. coli isolates were showed multiple drug resistance for three or more antibiotics. The result of this study, combined with data from previous studies in different countries, suggests that unhygienic practice of food handling and processing is major contributors to the dissemination of not only ESBL- E. coli but also MDR bacteria.…”

Section: Discussionmentioning

confidence: 72%

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Assessment of extended-spectrum β-lactamase (ESBLs) – producing Escherichia coli from minced meat of cattle and swab samples and hygienic status of meat retailer shops in Jimma town, Southwest Ethiopia

et al. 2019

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BackgroundThe impact of animals sources of food as a possible reservoir for extended-spectrum β-lactamase (ESBL) - Producing E. coli, and the dissemination of such strains into the food production chain need to be assessed. This study was aimed to assess the presence and antimicrobial susceptibility patterns of ESBLs - producing E. coli isolates from minced meat and environmental swab samples at meat retailer shops of Jimma town, Southwest Ethiopia.MethodologyA cross-sectional descriptive study was conducted from March to June, 2016. A total of 168 minced meat and swab samples were first enriched by buffered peptone water (BPW) for overnight and streaked onto MacConkey agar. Double disk synergy (DDS) method was used for detection of ESBL-producing strains. A disk of amoxicillin + clavulanic acid (20/10 μg) was placed in the center of Mueller-Hinton agar plate, and cefotaxime (30 μg) and ceftazidime (30 μg) were placed at a distance of 20 mm from the central disk. Checklist was used to assess hygienic status of butcher shops and practices meat handlers.ResultsA total of 35 (20.80%) biochemically confirmed E. coli were obtained from 168 samples. Of these, 21 (23.9%) of them were detected from 88 minced meat and the other 14 (17.5%) from 80 swab samples taken from butcher’s hand, knives, chopping board and protective clothing. From 35 E. coli isolates, 7(20%) of them were confirmed as ESBL- producers. Among ESBL- producing strains, 85.7% were resistant for cefotaxime and ceftriaxone and 71.4% for ceftazidime. Among non-ESBLs-producing strains only seven isolates were resistant to third generation cephalosporin. All E. coli isolates were resistant to ampicillin, penicillin and erythromycin, and susceptible to amikacin. Poor hygienic status of butcher shops and unhygienic practice of meat handlers were observed.ConclusionThe detections of ESBLs- producing strains could be contributed for the increment of multi drug resistant isolates. This study also concluded that, unhygienic meat handling and processing practice can contribute for contaminations of meat. Thus, strategies should be planned and implemented to improve the knowledge and practice of butchers about handling and processing of meat.

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Section: Discussioncontrasting

confidence: 90%

Section: Discussionmentioning

confidence: 72%

Assessment of extended-spectrum β-lactamase (ESBLs) – producing Escherichia coli from minced meat of cattle and swab samples and hygienic status of meat retailer shops in Jimma town, Southwest Ethiopia

et al. 2019

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“…This is similar to the findings from Luvsansharav U et al in Japon (69.3%) in 2012 on Prevalence of and risk factors associated with fecal carriage of CTX-M blactamase-producing Enterobacteriacae in rural Thai communities [29]. A high prevalence above 50% has also been observed among healthy individuals (57%) for Escherichia coli in a study conducted by Saleem et al in 2017 in Pakistan [30]. On the other hand, studies conducted by Michaud et al in Senegal in 2014 among farm workers showed a low digestive carrying rate (9.4%) [18].…”

Section: Discussionsupporting

confidence: 89%

High Carrying Rate of Extended-Spectrum Beta-Lactamase (ESBL) Producing Enterobacteriaceae by Slaughterhouse Workers in Lomé, Togo in 2019

Godonou

Gbeasor-Komlanvi

Tsogbale

et al. 2020

MRJI

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Introduction: Extended-spectrum beta-lactamase-producing Enterobacteriaceae (ESBL) represent a real public health concern because of their spread. The role of agri-food chains in transmitting of digestive ESBL-producing bacterial strains in the community, was demonstrated but little work was done in our settings (Togo, west Africa). The aim of this study was to estimate the rate of digestive carrying ESBL producing enterobacteriacae in slaughterhouse workers in Lomé, Togo. Material and Methods: This was a cross-sectional study carried out in three slaughterhouses in Lomé. Fresh stools of 60 slaughterhouse workers and socio-demographic data were collected during the period of September to October 2019 after obtaining the consent of each participant. The bacterial strains of interest were isolated on the selective medium Purple Bromocresol + Ceftazidime at 6µg/l. UriselectÒ and API 20E media were used for identification. Antibiotic susceptibility test was performed in Mueller-Hinton agar plate diffusion method (Kirby Bauer technic) and according to CASFM-EUCAST recommendations. Results: The digestive carriage rate of ESBL producing enterobacteriacae among professionals of three slaughterhouses of Lomé was 80% (n=48/60). Escherichia coli was the main bacteria 78.2% (n = 43/55) followed by Klebsiella pneumoniae 16.4% (n = 9/55) and Enterobacter cloacae 5.4% (n = 3/55). The antibiotic profile of ESBL producing enterobacteriacae showed resistance to Amoxycillin + Clavulanic Acid (26%), Ticarcillin + Clavulanic Acid (86%), Piperacillin + Tazobactam (14%), Cefoxitin (7%) Ciprofloxacin (63%), Levofloxacin (49%), Nalidixic Acid (42%), Chloramphenicol (33%), Gentamicin (21%), Sulfamethoxazole-Trimetoprim (93%). These bacteria were 100% sensitive to Imipenem, Ertapenem, Amikacin and Fosfomycin. Conclusion: This study revealed a very high carriage rate of ESBL producing Enterobacteriacae among Slaughterhouse Workers in Lomé. It confirmed the major potential role of the agri-food chains in the spread of ESBL producing bacteria in the Community.

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“…Studies have shown that extended-spectrum β-lactamases (ESBL)—producing E . coli are resistant to several antibiotics especially penicillins and cephalosporins however, they are susceptible to cephamycins and carbapenems [28]. In our study, resistance rates to the third and fourth generation cephalosporins were quite low and observed only among poultry-sellers at live bird markets.…”

Section: Resultsmentioning

confidence: 54%

Prevalence and risk factors for multi-drug resistant Escherichia coli among poultry workers in the Federal Capital Territory, Abuja, Nigeria

et al. 2019

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Background Antimicrobial resistance has emerged as a global health threat. Antimicrobial resistant Escherichia coli infections are associated with high morbidity and expenditure when compared with infections caused by susceptible strains. In Nigeria, antimicrobial drugs are readily available over-the-counter with potential for indiscriminate use by poultry farmers and eventual development of drug resistance. The objective of this study was to investigate prevalence and risk factors for multi-drug resistant E. coli among poultry workers (PW) in Abuja, Nigeria. Materials and methods A cross-sectional study was conducted among 122 randomly selected apparently healthy poultry workers (poultry-farmers/ sellers) in Municipal and Kuje Area Councils from December 2018 to April 2019. Data was collected on socio-demographics and exposure factors using a structured interviewer-administered questionnaire. E. coli was isolated and identified from stool samples of poultry workers. Antibiotic susceptibility testing was done using Kirby-Bauer disk diffusion method. Multidrug resistance (MDR) was defined as resistance to three or more classes of antimicrobials. Data was analyzed by computing proportions, prevalence odds-ratios (POR) and logistic regression at 5% significance level. Results Among PW, there were 121 males (99.2%). Mean age of the male workers was 30.6 ± 9.7years, 54.6% (n = 66) married, 57.9% (n = 70) had secondary education and 62.0%

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Phenotypic characterization of extended-spectrum-beta‑lactamase producing E. coli from healthy individuals, patients, sewage sludge, cattle, chickens and raw meat

Cited by 13 publications

References 16 publications

Assessment of extended-spectrum β-lactamase (ESBLs) – producing Escherichia coli from minced meat of cattle and swab samples and hygienic status of meat retailer shops in Jimma town, Southwest Ethiopia

Assessment of extended-spectrum β-lactamase (ESBLs) – producing Escherichia coli from minced meat of cattle and swab samples and hygienic status of meat retailer shops in Jimma town, Southwest Ethiopia

High Carrying Rate of Extended-Spectrum Beta-Lactamase (ESBL) Producing Enterobacteriaceae by Slaughterhouse Workers in Lomé, Togo in 2019

Prevalence and risk factors for multi-drug resistant Escherichia coli among poultry workers in the Federal Capital Territory, Abuja, Nigeria

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