Phenotypic characterization of type II collagen-induced arthritis in Wistar rats

Abstract

Abstract. The aim of the present study was to determine a more specific, efficient and simple method for the induction of collagen-induced arthritis (CIA) in rats. Different strains of rats were injected at the base of the tail with bovine type II collagen (CII) emulsified in incomplete Freund's adjuvant (IFA). The onset and severity of arthritis were evaluated by clinical assessment. The established CIA model was analyzed using a comprehensive examination of clinical, hematological, histological and radiological parameters. The results demonstrated that Wistar rats were the most susceptible strain to CIA followed by Wistar Furth rats, with Sprague Dawley rats being the least susceptible. Following primary and booster immunization, female Wistar rats developed severe arthritis, with an incidence of >83% and low variability in clinical signs. The development of arthritis was accompanied by a significantly elevated erythrocyte sedimentation rate compared with that in the control rats. The radiographic examination revealed bone matrix resorption, considerable soft tissue swelling, periosteal new bone formation and bone erosion in the arthritic joints of the CIA rats. Histopathologically, the synovial joints of CIA rats were characterized by synovial hyperplasia, pannus formation, marked cellular infiltration, bone and cartilage erosion and narrowing of the joint space. The administration of an intradermal injection of only 200 µg bovine CII emulsified in IFA at the base of the tail therefore leads to the successful development of a CIA rat model. This well-characterized CIA rat model could be specifically used to study the pathophysiology of human rheumatoid arthritis as well as to test and develop anti-arthritic agents for humans.

Atrial Fibrillation Promotion in a Rat Model of Rheumatoid Arthritis

“…Previous study suggested that female Wistar rats were greatly susceptible to CIA and had low variability in clinical signs. 9 Therefore, the CIA model was induced in female Wistar rats in this study.…”

β-Endorphin attenuates collagen-induced arthritis partially by inhibiting peripheral pro-inflammatory mediators

“…Paw arthritic signs characterized by edema and erythema were inspected daily following the CII/IFA injection. To evaluate the incidence and severity of arthritis, lesions on all four paws of each rat were graded by using an arthritic scoring system (13). Lesions were graded using a scale from 0 to 4 according to the extent of edema and erythema of the periarticular tissues; 16 was the potential maximum of the combined arthritic scores per animal.…”

Papel da exposição à Hidroquinona na artrite reumatoide experimental induzida pelo colágeno

“…Até o presente, os processos inflamatórios articulares foram bem caracterizados em modelos experimentais que mimetizam a AR humana. O desenvolvimento da AR vem sido bem descrito em uma ampla variedade de modelos animais com utilização de diversos estímulos, como colágeno, adjuvante de Freund, proteoglicanos, pristanos, avridinas, antígenos entre outros, que são capazes de simular/desencadear respostas celulares, bioquímicas, imunológicas e alterações histológicas que mimetizam em grande parte as condições patológicas da AR humana (DURIE, FAVA e NOELLE, 1994;BRAND et al, 1996;ZHANG et al, 2013;PINTO et al, 2015;SONG et al, 2015;BESSIS et al, 2017).…”

“…Neste contexto, o modelo experimental de artrite induzida por colágeno (AIC) apresenta características fisiopatológicas semelhantes à AR humana, além de ser amplamente utilizado para ilustrar os mecanismos patológicos relevantes na AR humana e para identificar potenciais agentes terapêuticos (DURIE, FAVA e NOELLE, 1994SONG et al, 2015;BESSIS et al, 2017). Em ambos tipos de artrite, células do sistema imune e dos tecidos sinoviais produzem uma série de citocinas inflamatórias e outros mediadores solúveis em uma cascata inflamatória.…”

“…Em roedores, AIC pode ser induzida pela imunização com emulsão de colágeno tipo II em adjuvante completo de Freund, que leva à produção de anticorpos específicos para colágeno tipo II (BRAND et al, 1996;BEVAART, VERVOORDELDONK e TAK, 2010;SONG et al, 2015). Fenotipicamente, animais com AIC apresentam proliferação incontrolada de sinoviócitos tipo B, infiltração de leucócitos nas articulações e elevada expressão local de NF-kB.…”