Phenotypic detection of MBL, Ampc beta-lactamase and carbapenemases in multi drug resistant isolates ofAcinetobacter baumannii

Hans, Richa; Bisht, Dakshina; Agarwal, Ritu; Irfan, Muhammad

doi:10.5958/2319-5886.2015.00058.2

Cited by 7 publications

(11 citation statements)

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“…Our results agree with those mentioned by Hans et al (2015), they found that all studied A. baumannii isolates obtained from Indian hospitals were resistant to gentamicin, erythromycin, trimethoprim/sulphamethaxole, piperacillin/tazobactam and ceftazidime. While they were sensitive to colistin and tigecycline [15]. Inaddition, our results agree with the reported results in Iran among MDR A. baumannii in which 91.2% were resistant to ceftazidime [17].…”

Section: Discussionsupporting

confidence: 93%

“…Inaddition, our results agree with the reported results in Iran among MDR A. baumannii in which 91.2% were resistant to ceftazidime [17]. In the present study, sputum samples (61, 54.5%) were the main source of A. baumannii, followed by (20, 17.9%) from blood samples, (17,15.2%) wound swab, then (5, 4.5%) CSF, (4, 3.6%) C.V. Line, (3, 2.7%) pus, (1, 0.9%) urine and (1, 0.9%) pleural fluid. In previous study conducted in Coimbatore, South India isolation rate was found to be 73% from respiratory secretions [18].…”

Section: Discussionsupporting

confidence: 92%

“…On the other hand, all (112, 100%) of A. baumannii isolates where sensitive to Colistin and Polymixin B. Our results agree with those mentioned by Hans et al (2015), they found that all studied A. baumannii isolates obtained from Indian hospitals were resistant to gentamicin, erythromycin, trimethoprim/sulphamethaxole, piperacillin/tazobactam and ceftazidime. While they were sensitive to colistin and tigecycline [15].…”

Section: Discussionsupporting

confidence: 91%

“…A. baumannii infections tend to occur more frequently in patients on broad spectrum antibiotics, immune-compromised individuals and with underlying diseases and those exposed to invasive procedures [14]. Newer beta-lactamases that hydrolyse cephamycins, cephalosporins, monobactams and carbapenems are of increased concern as they limit therapeutic options leading to treatment failures and poor prognosis [15]. Different mechanisms contributing to resistance including; carbapenemase production, modification of penicillin-binding proteins (PBPs), loss of porins, and/or altered efflux pump activity [16].…”

Section: Discussionmentioning

confidence: 99%

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Phenotypic Characterization of Extended-Spectrum Beta-Lactamases and Metallo-Beta-Lactamase of Multi Drug Resistant Acinetobacter baumannii Causing Nosocomial Infections in Erbil City

Smail¹,

Al-Otrachi²

2020

MJS

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Background: Resistance to broad‑spectrum beta‑lactams, mediated by extended‑spectrum beta‑lactamases, and metallo‑beta‑lactamase enzymes, is an increasing problem worldwide. The main aim is to study phenotypic characterization of extended‑spectrum beta‑lactamases and metallo‑beta‑lactamase multidrug resistant Acinetobacter baumannii in Erbil City. Materials and Methods: A total of 112 Acinetobacter baumannii isolations were collected from patients of all age groups from clinical specimens sputum, blood, pus, wound swab, urine and body fluids (Pleural fluid and cerebrospinal fluid) collected from different medical wards and intensive care unit departments of hospitals in Erbil City for a period of one year from march 2018--march 2019. Isolates were tested for the presence of extended‑spectrum beta‑lactamases and metallo‑beta‑lactamase. Detection of extended‑spectrum beta‑lactamases was done by the combined disk diffusion method according to Clinical and Laboratory Standards Institute guidelines, while metallo‑beta‑lactamase was detected by meropenem and imipenem combined with ethylenediaminetetraacetic acid disk method. Results: 25% (28) Acinetobacter baumannii isolates were positive for extended‑spectrum beta‑lactamases, while 100 % (112) were metallo‑beta‑lactamase producers. Conclusion: Acinetobacter baumannii is becoming a global medical challenge due to the emergence of multi-drug resistance. Newer beta lactamase is a matter of concern as they are developing rapidly and lead to treatment failure. Carbapenems are known to be effective therapeutic agents for Acinetobacter baumannii infections and its resistance limits the use to polymyxins and colistin. Several new medicines are still in research and combination of drug therapy is being currently used in the hospitals together with ours to treat multidrug resistant Acinetobacter baumannii infections.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

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Phenotypic Characterization of Extended-Spectrum Beta-Lactamases and Metallo-Beta-Lactamase of Multi Drug Resistant Acinetobacter baumannii Causing Nosocomial Infections in Erbil City

Smail¹,

Al-Otrachi²

2020

MJS

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“…have been reported from the same hospital by Parajuli et al 24 However, in an Indian study, the higher rate (56%) of AmpC producing A. baumannii was documented. 26 Carbapenem-resistant Acinetobacter baumannii (CRAB) is included in priority 1 (ie, critical) of a global priority list of antibiotic-resistant bacteria to guide research, discovery, and development of new drugs by the World Health Organization. 27 Although there are different mechanisms for carbapenem resistance, the production of carbapenemases enzyme is the most effective mechanism.…”

Section: Discussionmentioning

confidence: 99%

Burden of Multidrug-Resistant Acinetobacter baumannii Infection in Hospitalized Patients in a Tertiary Care Hospital of Nepal

Yadav¹,

Bhujel

Hamal

et al. 2020

IDR

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Rapid identification of carbapenemases by CarbAcineto NP test and the rate of beta-lactamases among Acinetobacter baumannii from a teaching hospital

Rao¹,

Urs²,

Chitharagi³

et al. 2022

IJM

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Background and Objectives: Acinetobacter baumannii has emerged as a major organism accounting for hospital acquired infections particularly in intensive care units. Due to production of different kinds of beta lactamases these bacteria have developed drug resistance rendering the treatment of such infections very difficult and expensive. Rapid identification of A. baumannii producing such beta-lactamases is the need of the hour in reducing morbidity and mortality associated with A. baumannii infections. Materials and Methods: A. baumannii was isolated from clinical samples like endotracheal aspirates, sputum, urine, exu- dates using standard culture techniques. Identification and drug sensitivity was done using Vitek 2 system. All the isolates were subjected to detection of ESBLs using phenotypic confirmatory test, plasmid mediated AmpC beta- lactamase by AmpC disc test, Carbapenemase production by CarbAcineto NP Test and Modified hodge method. Results: 149 A. baumannii isolates were analysed for antimicrobial susceptibility and various beta-lactamase production. Results were evaluated for statistical significance using Chi-Square and P value. 81.8% of isolates were from male patients with majority of them above 50 years of age. 88.5% of samples were from ventilator associated pneumonia patients. 83.8% of isolates were sensitive to tigecycline. Only 10% to 12% of isolates were sensitive to carbapenems. 23.4% of isolates were ESBL producers and 46.9% of them were AmpC producers. Modified Hodge test method identified 63.7% of A. baumannii as carbapenemase producers where as CarbAcineto NP test identified 63% and exibiting 94.74% sensitivity, 93.22% specificity when compared to Modified Hodge test. Conclusion: Multidrug resistant Acinetobacter spp. is on the rise. Present study showed that high percentage of drug resis- tance in A. baumannii could be due to production of ESBLs, AmpC and carbapenemases. Among all beta lactamases car- bapenemase producers are more and quickly raising in A. baumannii. Rapid, cost effective assay which can be adopted in all clinical laboratories is critical to prevent their further transmission particularly in hospital environment.

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Phenotypic detection of MBL, Ampc beta-lactamase and carbapenemases in multi drug resistant isolates ofAcinetobacter baumannii

Cited by 7 publications

References 0 publications

Phenotypic Characterization of Extended-Spectrum Beta-Lactamases and Metallo-Beta-Lactamase of Multi Drug Resistant Acinetobacter baumannii Causing Nosocomial Infections in Erbil City

Phenotypic Characterization of Extended-Spectrum Beta-Lactamases and Metallo-Beta-Lactamase of Multi Drug Resistant Acinetobacter baumannii Causing Nosocomial Infections in Erbil City

<p>Burden of Multidrug-Resistant <em>Acinetobacter baumannii</em> Infection in Hospitalized Patients in a Tertiary Care Hospital of Nepal</p>

Rapid identification of carbapenemases by CarbAcineto NP test and the rate of beta-lactamases among Acinetobacter baumannii from a teaching hospital

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