Background: In the present study, we have set a goal, to study the genetic variability and phylogenetic affinities of 26 spontaneous Vicia sativa L. accessions collected in Algeria, as part of the recovery and enhancement of the local genetic heritage and its integration into the breeding and varietal creation programs. Methods: Morphological traits, performance parameters and phenological stages of each accession were tracked during an experimental test in pots under greenhouse conditions from December 29, 2019 to June 1, 2020. An eco-geographical characterization of samples was also carried out through geographical coordinates. On the other side and for the first time in Algeria, soil analyses of each site were undertaken. Result: The phylogenetic study of accessions confirm the taxonomic complexity of the group V. sativa L. According to the principal component analyses and kruskal-Wallis test, the most segregating variables with the highest values and the largest K variance ratios are most of the attributes associated with the flower, pod and seed. The association of the two dissimilarity matrix carried out by a Mantel test between the morphological and eco-geographic parameters showed a significant effect with a p value = 0.004 at the threshold of alpha=0.05 and a correlation coefficient r (AB) = 0.156.