Phenotypic methods of greater accuracy to detect the mecA gene product for the recognition of MRSA in resource constraint settings

Rasheed, M U; Ahmed, Z. Baba

doi:10.1016/s1995-7645(10)60178-2

Cited by 7 publications

(9 citation statements)

References 16 publications

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“…[17] also reported 22% carriage rate of MRSA among children of south Texas. The findings of this study was in agreement with earlier studies reported from different parts of the world [10,12]. …”

Section: Discussionsupporting

confidence: 94%

“…Here, MRSA was detected using Cefoxitin (30μg) disc which has high efficiency to detect MRSA and has been used as an alternative to PCR in resource constrain areas [12,16]. Alfaro et al .…”

Section: Discussionmentioning

confidence: 99%

“…Detection of Methicillin resistant S. aureus (MRSA) was carried out using Cefoxitin disc as described earlier [12]. Briefly, all the isolates were subjected to Cefoxitin disc diffusion test using a 30μg disc.…”

Section: Methodsmentioning

confidence: 99%

“…Plates were incubated at 35 0 C for 18–24 hours and inhibition zone diameters (mm) were measured. An inhibition zone diameter of ≤ 21mm was reported as methicillin/oxacillin resistant and ≥ 22mm was considered as methicillin /oxacillin sensitive[12]. …”

Section: Methodsmentioning

confidence: 99%

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Prevalence and antibiotic susceptibility pattern of methicillin-resistant Staphylococcus aureus (MRSA) among primary school children and prisoners in Jimma Town, Southwest Ethiopia

Kejela

Bacha

2013

Ann Clin Microbiol Antimicrob

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BackgroundStaphylococcus aureus infections are increasingly reported from both health institutions and communities around the world. In particular, infections due to methicillin-resistant Staphylococcus aureus (MRSA) strains have been detected worldwide. If MRSA becomes the most common form of S. aureus in a community, it makes the treatment of common infections much more difficult. But, report on the current status of community acquired MRSA in the study area is scanty.MethodsCommunity-based cross sectional study was conducted to evaluate the current prevalence and antibiotic susceptibility pattern of MRSA among primary school children and prisoners in Jimma town. MRSA was detected using Cefoxitin (30μg) disc; and epidemiologic risk factors were assessed using pre-designed questionnaires distributed to the children’s parents and prisoners. A total of 354 nasal swabs were collected from primary school children and prisoners from December 2010 to March 2011 following standards microbiological methods.ResultsA total of 169 S. aureus isolates were recovered. The overall prevalence of MRSA among the study population was 23.08 % (39/169). Specifically, the prevalence of MRSA among primary school children and prisoners were 18.8% (27/144) and 48% (12/25), respectively. The isolated S. aureus and MRSA displayed multiple drug resistance (MDR) to 2 to 10 antibiotics. The most frequent MDR was Amp/Bac/Ery/Pen/Fox (resistance to Ampicillin, Bacitracin, Erythromycin, Penicillin, and Cefoxitin).ConclusionThe present study revealed that MRSA could be prevalent in the healthy community, transmitted from hospital to the community. The high distribution of MRSA could be favored by potential risk factors. Thus, for comprehensive evaluation of the current prevalence of MRSA and design control measures, consideration need to be given to the healthy community besides data coming from health institutions.

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Section: Discussionsupporting

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Prevalence and antibiotic susceptibility pattern of methicillin-resistant Staphylococcus aureus (MRSA) among primary school children and prisoners in Jimma Town, Southwest Ethiopia

Kejela

Bacha

2013

Ann Clin Microbiol Antimicrob

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“…The search for newer sources of antibiotics is a global challenge for preoccupying research institutions, pharmaceutical companies and academia, since many infectious agents are becoming resistant to synthetic drugs [24][25][26][27][28] . R ecently published guidelines have recommended the use of vancomycin in the management of severe MRSA infection [29] , but it was reported that the vancomycin resistant S. aureus was emerging in northern India [10] .…”

Section: Discussionmentioning

confidence: 99%

Isolation of a small molecule with anti–MRSA activity from a mangrove symbiont Streptomyces sp. PVRK–1 and its biomedical studies in Zebrafish embryos

Kannan

Iniyan²,

Prakash

2011

Asian Pacific Journal of Tropical Biomedicine

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A Multiplex PCR Assay for the Rapid and Sensitive Detection of Methicillin‐Resistant Staphylococcus aureus and Simultaneous Discrimination of Staphylococcus aureus from Coagulase‐Negative Staphylococci

Xu¹,

Liu²,

Liu³

et al. 2012

Journal of Food Science

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Methicillin-resistant Staphylococcus aureus (MRSA) is a global health concern, which had been detected in food and food production animals. Conventional testing for detection of MRSA takes 3 to 5 d to yield complete information of the organism and its antibiotic sensitivity pattern. So, a rapid method is needed to diagnose and treat the MRSA infections. The present study focused on the development of a multiplex PCR assay for the rapid and sensitive detection of MRSA. The assay simultaneously detected 4 genes, namely, 16S rRNA of the Staphylococcus genus, femA of S. aureus, mecA that encodes methicillin resistance, and one internal control. It was rapid and yielded results within 4 h. The analytical sensitivity and specificity of the multiplex PCR assay was evaluated by comparing it with the conventional method. The analytical sensitivity of the multiplex PCR assay at the DNA level was 10 ng DNA. The analytical specificity was evaluated with 10 reference staphylococci strains and was 100%. The diagnostic evaluation of MRSA was carried out using 360 foodborne staphylococci isolates, and showed 99.1% of specificity, 96.4% of sensitivity, 97.5% of positive predictive value, and 97.3% of negative predictive value compared to the conventional method. The inclusion of an internal control in the multiplex PCR assay is important to exclude false-negative cases. This test can be used as an effective diagnostic and surveillance tool to investigate the spread and emergence of MRSA.

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Phenotypic methods of greater accuracy to detect the mecA gene product for the recognition of MRSA in resource constraint settings

Cited by 7 publications

References 16 publications

Prevalence and antibiotic susceptibility pattern of methicillin-resistant Staphylococcus aureus (MRSA) among primary school children and prisoners in Jimma Town, Southwest Ethiopia

Prevalence and antibiotic susceptibility pattern of methicillin-resistant Staphylococcus aureus (MRSA) among primary school children and prisoners in Jimma Town, Southwest Ethiopia

Isolation of a small molecule with anti–MRSA activity from a mangrove symbiont Streptomyces sp. PVRK–1 and its biomedical studies in Zebrafish embryos

A Multiplex PCR Assay for the Rapid and Sensitive Detection of Methicillin‐Resistant Staphylococcus aureus and Simultaneous Discrimination of Staphylococcus aureus from Coagulase‐Negative Staphylococci

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