Phenotypic Modulation of Biofilm Formation in a Staphylococcus epidermidis Orthopedic Clinical Isolate Grown Under Different Mechanical Stimuli: Contribution From a Combined Proteomic Study

Bottagisio, Marta; Barbacini, Pietro; Bidossi, Alessandro; Torretta, Enrica; deLancey-Pulcini, Elinor; Gelfi, Cecilia; James, Garth A.; Lovati, Arianna B.; Capitanio, Daniele

doi:10.3389/fmicb.2020.565914

Cited by 6 publications

(5 citation statements)

References 66 publications

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“…A possible explanation for this observation is the absence of host-derived bridging molecules (i.e., fibrinogen). In their absence, cells must produce intercellular adhesion factors, whose expression is probably conditioned to extreme environmental stresses, such as starvation [ 32 , 33 ]. On the other hand, staphylococci express an impressive arsenal of surface adhesins, which allows them to scavenge host-derived polymeric molecules and to exploit them as an extracellular matrix [ 1 , 8 , 11 , 13 , 21 ].…”

Section: Discussionmentioning

confidence: 99%

Host Environment Shapes S. aureus Social Behavior as Revealed by Microscopy Pattern Formation and Dynamic Aggregation Analysis

et al. 2022

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Understanding how bacteria adapt their social behavior to environmental changes is of crucial importance from both biological and clinical perspectives. Staphylococcus aureus is among the most common infecting agents in orthopedics, but its recalcitrance to the immune system and to antimicrobial treatments in the physiological microenvironment are still poorly understood. By means of optical and confocal microscopy, image pattern analysis, and mathematical modeling, we show that planktonic biofilm-like aggregates and sessile biofilm lifestyles are two co-existing and interacting phases of the same environmentally adaptive developmental process and that they exhibit substantial differences when S. aureus is grown in physiological fluids instead of common lab media. Physicochemical properties of the physiological microenvironment are proposed to be the key determinants of these differences. Besides providing a new tool for biofilm phenotypic analysis, our results suggest new insights into the social behavior of S. aureus in physiological conditions and highlight the inadequacy of commonly used lab media for both biological and clinical studies of bacterial development.

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Section: Discussionmentioning

confidence: 99%

Host Environment Shapes S. aureus Social Behavior as Revealed by Microscopy Pattern Formation and Dynamic Aggregation Analysis

et al. 2022

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“…50,52 Some mechanical stimuli may even support biofilm development as a defense strategy in a hostile environment. 53 As the biofilm grows and matures, bacteria are embedded in the selfproduced ECM whose composition can change in response to mechanical forces, significantly impacting the overall stability of biofilms. These mechanobiological interactions thus occur at all three scales: bacteria, biofilm, and implant, as illustrated in Figure 2.…”

Section: Multiscale Mechanobiology Of Biofilm Developmentmentioning

confidence: 99%

“…Studies have shown that bacteria sense mechanical stimuli through different receptors and effectors, which enable them to adapt, deform, and attach to or detach from a surface 50,52 . Some mechanical stimuli may even support biofilm development as a defense strategy in a hostile environment 53 . As the biofilm grows and matures, bacteria are embedded in the self‐produced ECM whose composition can change in response to mechanical forces, significantly impacting the overall stability of biofilms.…”

Section: Multiscale Mechanobiology Of Biofilm Developmentmentioning

confidence: 99%

Mechanobiology of bacterial biofilms: Implications for orthopedic infection

Blondel,

Machet,

Wildemann

et al. 2024

Journal Orthopaedic Research

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Postoperative bacterial infections are prevalent complications in both human and veterinary orthopedic surgery, particularly when a biofilm develops. These infections often result in delayed healing, early revision, permanent functional loss, and, in severe cases, amputation. The diagnosis and treatment pose significant challenges, and bacterial biofilm further amplifies the therapeutic difficulty as it confers protection against the host immune system and against antibiotics which are usually administered as a first‐line therapeutic option. However, the inappropriate use of antibiotics has led to the emergence of numerous multidrug‐resistant organisms, which largely compromise the already imperfect treatment efficiency. In this context, the study of bacterial biofilm formation allows to better target antibiotic use and to evaluate alternative therapeutic strategies. Exploration of the roles played by mechanical factors on biofilm development is of particular interest, especially because cartilage and bone tissues are reactive environments that are subjected to mechanical load. This review delves into the current landscape of biofilm mechanobiology, exploring the role of mechanical factors on biofilm development through a multiscale prism starting from bacterial microscopic scale to reach biofilm mesoscopic size and finally the macroscopic scale of the fracture site or bone–implant interface.

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“…Several virulence factors have been defined in this review; we mostly focused on biofilm formation as a significant virulence factor (Cheung et al, 2010). Moreover, the invasion of human immune defense by the bacteria becomes a nonharmful function as soon as the life of bacteria as a commensal on, such as the human skin (Lood et al, 2015), and this ability is still not completely understood; consequently, S. epidermidis is occasionally referred to as "the accidental pathogen" (Bottagisio et al, 2020).…”

Section: Virulence Factorsmentioning

confidence: 99%

Staphylococcus epidermidis biofilms: Functional molecules; relation to virulence and the host immune response

Abdul¹,

Raheem²,

Abdulrazaq³

et al. 2021

JLBSR

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Staphylococcus epidermidis is the most significant nosocomial pathogen related to people with vulnerable frameworks such as malignant growth patients, neonates, and foreign body embedded materials such as heart valves. A few virulence factors in S. epidermidis can cause host damage in comparison to Staphylococcus aureus. In spite of that, the key roles of S. epidermidis virulency rely on biofilm formation, bacterial biofilm is essential for the pathogenesis by encouraging microorganisms to consist shape networks of assurance rather than free planktonic cells, hence resistance to antibacterial agents, and medically uninsured problems by colonizing medical indwelling, making the disease long span, and difficult to treat. The National Institute of Health (NIH) reported 65-80% of bacterial illnesses are biofilm formed, thus making numerous passing wellbeing additional costs. Therefore, the biofilms establishing on the susceptible hosts' tissues demonstrate; preventing antibiotics efficient treatment, protecting against host defense mechanisms, and announce the bacteria virulence determinants manifesting.

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Phenotypic Modulation of Biofilm Formation in a Staphylococcus epidermidis Orthopedic Clinical Isolate Grown Under Different Mechanical Stimuli: Contribution From a Combined Proteomic Study

Cited by 6 publications

References 66 publications

Host Environment Shapes S. aureus Social Behavior as Revealed by Microscopy Pattern Formation and Dynamic Aggregation Analysis

Host Environment Shapes S. aureus Social Behavior as Revealed by Microscopy Pattern Formation and Dynamic Aggregation Analysis

Mechanobiology of bacterial biofilms: Implications for orthopedic infection

Staphylococcus epidermidis biofilms: Functional molecules; relation to virulence and the host immune response

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