Phenotypic rescue of the albino mutation in the medakafish (Oryzias latipes) by a mouse tyrosinase transgene

Hyodo-Taguchi, Yasuko; Winkler, Christoph; Kurihara, Y; Schartl, Angelika; Schartl, Manfred

doi:10.1016/s0925-4773(97)00128-7

Cited by 35 publications

(17 citation statements)

References 30 publications

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“…Single cells were obtained by trypsinization, rinsed, and resuspended in cell transplantation medium (TM: 100 mM NaCl͞5 mM KCl͞5 mM Hepes, pH 7.1) for microinjection within 2 h at room temperature. Outbred albino medaka strains (i1 and i3) (23,24) were used as the host. Embryos were collected shortly after fertilization and dechorionated as described (17,21).…”

Section: Methodsmentioning

confidence: 99%

Production of medakafish chimeras from a stable embryonic stem cell line

Hong

Winkler

Schartl

1998

Proc. Natl. Acad. Sci. U.S.A.

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163

167

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Embryonic stem (ES) cell lines provide a unique tool for introducing targeted or random genetic alterations through gene replacement, insertional mutagenesis, and gene addition because they offer the possibility for in vitro selection for the desired, but extremely rare, recombinant genotypes. So far only mouse blastocyst embryos are known to have the competence to give rise to such ES cell lines. We recently have established a stable cell line (Mes1) from blastulae of the medakafish (Oryzias latipes) that shows all characteristics of mouse ES cells in vitro. Here, we demonstrate that Mes1 cells also have the competence for chimera formation; 90% of host blastulae transplanted with Mes1 cells developed into chimeric fry. This high frequency was not compromised by cryostorage or DNA transfection of the donor cells. The Mes1 cells contributed to numerous organs derived from all three germ layers and differentiated into various types of functional cells, most readily observable in pigmented chimeras. These features suggest the possibility that Mes1 cells may be a fish equivalent of mouse ES cells and that medaka can be used as another system for the application of the ES cell technology.

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Section: Methodsmentioning

confidence: 99%

Production of medakafish chimeras from a stable embryonic stem cell line

Hong

Winkler

Schartl

1998

Proc. Natl. Acad. Sci. U.S.A.

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163

167

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“…Tyrosinase genes have been described in numerous vertebrates (Inagaki et al 1994;reviewed in Ferguson and Kidson 1997) including the zebrafish (GenBank Accession No: AJ250302). Interestingly, while pigment rescue studies have been conducted using tyrosinase transgenes (Beermann et al 1990(Beermann et al , 1992Hyodo-Taguchi et al 1997) and tyrosinase gene promoter activity has been assayed using various marker genes (Kluppel et al 1991;Tief et al 1996Tief et al , 1997Schmidt et al 1998), the spatiotemporal pattern of endogenous tyrosinase gene transcription has not been analysed in whole embryos in a vertebrate. Here we describe the spatiotemporal pattern of the zebrafish tyrosinase gene by whole-mount in situ transcript hybridisation and we report the detection of active tyrosinase enzyme approximately 3 h before the onset of pigmentation.…”

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confidence: 99%

Tyrosinase gene expression in zebrafish embryos

Camp¹,

Lardelli²

2001

Development Genes and Evolution

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The enzyme tyrosinase is required for the conversion of tyrosine into the pigment melanin. Thus, tyrosinase gene expression is a useful marker for studying the differentiation of melanin-expressing cells during embryogenesis. We describe the spatiotemporal pattern of transcription of the tyrosinase gene and the presence of active enzyme in whole embryos of the zebrafish, Danio rerio. At 16.5 h post-fertilisation the tyrosinase gene is transcribed in the dorsal extremity of the developing retinal pigment epithelium, approximately 7 h before visible pigmentation. Shortly thereafter, transcription in neural crest-derived melanocytes is first observed dorsolateral to the mesencephalon and diencephalon and the posterior hindbrain/anterior spinal cord. A wave of gene activation and cell migration is then observed moving towards the posterior of the animal. DOPA staining for tyrosinase activity shows the presence of active enzyme in embryos at least 3 h before visible pigmentation.

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“…The molecular mechanism of albinism of flounder is poorly understood and has long been a hot research topic in many laboratories throughout the world. As many different genetic defects can lead to albinism (Hyodo-Taguchi et al 1997), the cloning of fish albinism-related genes are of particular economic importance. Japanese flounder (class Actinopterygii, family Pleuronectiformes, genus Paralichthyidae) is a commercially important species in both the fishery and aquaculture industries in Korea, Japan and China (Kurokawa and Suzuki 1998;Yamamoto 1999).…”

Section: Introductionmentioning

confidence: 99%

Cloning and characterization of a novel albinism-related zinc finger protein gene in Japanese flounder

Wang

Lin

Zou

et al. 2007

Fish Physiol Biochem

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A novel zinc finger protein (ZFP) gene of the Japanese flounder, Paralichthys olicaceus, has been isolated by differential mRNA display (DD). This technique was utilized to simultaneously compare the mRNA transcript populations from pigmented skins of the ocular side of normal Japanese flounder (termed N1 skin) and non-pigmented skins of the ocular side of albino Japanese flounder (termed A1 skin). A reproducibly differentially expressed band was isolated and cloned from N1 skin tissue. The isolated 917-bp DD fragment (termed EST917) was screened and sequenced. Sequence analysis of EST917 revealed that it contains a complete open reading frame and encodes a polypeptide of 123 amino acids (named ZFP123), with a predicted molecular weight of 13.293 kDa. Expression profile analysis by a reverse transcription-PCR (RT-PCR) technique revealed the expression of the ZFP123 gene in N1 skin and its lack of expression in A1 skin; it was expressed, however, in both blind sides of normal Japanese flounder (termed N2 skin) and the blind side of albino Japanese flounder (termed A2 skin) in a very similar manner. ZFP123 mRNA was found to be highly expressed in muscle, eye, brain and liver, but weakly expressed or undetectable in the gill and fat in both normal and albino Japanese flounders. The ZFP123 of the Japanese flounder shows a high homology to those ZFPs of other species, and the phylogenetic relationship among 14 sequences isolated from other species are illustrated.

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Phenotypic rescue of the albino mutation in the medakafish (Oryzias latipes) by a mouse tyrosinase transgene

Cited by 35 publications

References 30 publications

Production of medakafish chimeras from a stable embryonic stem cell line

Production of medakafish chimeras from a stable embryonic stem cell line

Tyrosinase gene expression in zebrafish embryos

Cloning and characterization of a novel albinism-related zinc finger protein gene in Japanese flounder

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