Phenotypic Selection Assisted by Molecular Markers for Scab Resistance in Apple

Botez, C.; Sestraş, Radu E.; Ardelean, M.; Pamfil, Doru; Patrascu, Bianca; Sestraș, Adriana F.

doi:10.17660/actahortic.2009.814.130

Cited by 3 publications

(1 citation statement)

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“…After PCR analyses, "1" was given if there were DNA bands on the gels and "0" was given if there were no bands; DNA bands were scored in this way, and RAPD analyses were carried out on polymorphic bands by applying 219 Table 1. Primers used in the RAPD-PCR reactions and their Tm degrees In the past studies using RAPD-PCR technique including Desmodium (Rahman et al, 2017), Calendula (Baciu et al, 2013) Vigna mungo (Arulbalachandran et al, 2010), Velezia (Poyraz et al, 2012), Hordeum vulgare (Olgun et al, 2015), Prunus (Casas et al, 1999), Malus (Botez et al, 2009), Pyrus (Monte-Corvo et al, 2000, Olea europaea ssp. oleaster (Sesli and Yegenoğlu, 2017), and Brassica napus (Özbek and Gıdık, 2013) were reported with respect to genetic diversity and phylogenetic analysis of many plant species and populations.…”

Section: Data and Phylogenetic Analysismentioning

confidence: 99%

Molecular Characterization of Vitex agnus-castus L. (Verbenaceae) Populations Grown in Aydin, Turkey

2019

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In this study, we performed a genetic diversity analysis using RAPD markers for some Vitex agnus-castus populations grown in Aydin, Turkey. Total genomic DNA isolation from the leaves of Vitex agnus-castus was performed using a commercial kit. Seven RAPD primers (OPA-02, OPA-05, OPA-13, OPA-15, OPA-16, OPA-18, OPA-20) were used to determine genetic diversity among populations. Polymerase Chain Reaction (PCR) was performed with all genomic DNA samples and primers. PCR products were run in agarose gel electrophoresis and visualized under UV light. The amplified products were scored as bands (1) and no bands (0) for all gel images and their matrix files were generated. A total of 36 characters were obtained from the primers. Phylogenetic relationships and genetic distances between the cultivars were calculated by using the PAUP* (Phylogenetic Analysis Using Parsimony and other methods) program. According to PAUP analysis, the closest genetic distances were between Çine pink flower and Çakmar purple flower, and Çakmar pink flower and Çakmar purple flower populations with a value of 0.05556; and the greatest genetic distance was between Çakmar pink flower and Köşk purple flower populations with a value of 0.36111. In the phylogenetic analysis obtained using UPGMA algorithms, the phylogenetic tree consisted of four groups. The results suggest that RAPD markers are useful tools for determining genetic relationships among Vitex agnus-castus genotypes.

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Section: Data and Phylogenetic Analysismentioning

confidence: 99%

Molecular Characterization of Vitex agnus-castus L. (Verbenaceae) Populations Grown in Aydin, Turkey

2019

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The role of Molecular Markers in Improvement of Fruit Crops

et al. 2010

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Markers have been used over the years for the classification of plants. Markers are any trait of an organism that can be identified with confidence and relative easy, and can be followed in a mapping population on another hand markers be defined as heritable entities associated with the economically important trait under the control of polygenes. Morphological markers can be detected with naked eye (naked eye polymorphism) or as difference in physical or chemical properties of the macromolecules. In other words, there are two types of genetic markers viz. morphological markers or naked eye polymorphism and non-morphological markers or molecular markers. Morphological markers include traits such as plant height, disease response, photoperiod, sensitivity, shape or colour of flowers, fruits or seeds etc. Molecular markers include biochemical constituents. Morphological markers have many limitations for being used as markers particularly in fruit crops because of long generation time and large size of fruit trees besides being influenced by environment. Consequently, molecular markers could be appropriate choice to study and preserve the diversity in any germplasm. Molecular markers have diverse applications in fruit crop improvement, particularly in the areas of genetic diversity and varietal identification studies, gene tagging, disease diagnostics, pedigree analysis, hybrid detection, sex differentiation and marker assisted selection.

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