Phenotypic speciation of enterococci with special reference to prevalence, virulence and antimicrobial resistance

Manavalan, Jayavarthinni; Kannaiyan, Kavitha; Velayutham, Abarna; Vadivel, Sindhanai; Kuthalaramalingam, Sethumadhavan

doi:10.18203/2320-6012.ijrms20150802

Cited by 7 publications

(4 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the current study, 57 clinical enterococcal isolates were recognized by phenotypic methods as E. faecalis (49 isolates) and E. faecium (eight isolates). The phenotypic prevalence of enterococci in this study is consistent with reports from many studies indicating Enterococcus faecalis as the most common cause of enterococcal infections (20,21).…”

Section: Discussionsupporting

confidence: 92%

Loop-Mediated Isothermal Amplification Assay for Identification of Clinical Enterococcus Species

Miankouhi

Malakouti

Mirghafourvand

et al. 2019

Arch Clin Infect Dis

View full text Add to dashboard Cite

Background: Enterococci are recognized as a cause of nosocomial infections and a major public health problem. The reliable identification to the species level of enterococci should be considered. Objectives:The study aimed to develop a LAMP assay for the rapid and accurate detection of Enterococcus faecalis and E. faecium. Methods: In total, 57 enterococcal isolates from UTI patients were identified using conventional microbiological methods. Two sets of specific primers were designed for E. faecalis and E. faecium targeting the mtlf and efmC genes, respectively. The LAMP assays were conducted using specific primers, dNTPs, MgSO4, Bst DNA polymerase, and templates. Results: The results of phenotypic testing indicated that of the 57 enterococcal isolates, 49 (85.9%) were identified as E. faecalis and eight (14.1%) as E. faecium. The optimal reaction temperatures in the LAMP assays were 60 and 61ºC for the detection of E. faecalis and E. faecium, respectively. All the 57 enterococcal isolates were identified as E. faecalis by the LAMP assay. Conclusions: The present study highlights the importance of the LAMP assay as a rapid and confirmatory tool for the identification of clinical Enterococcus spp.

show abstract

Section: Discussionsupporting

confidence: 92%

Loop-Mediated Isothermal Amplification Assay for Identification of Clinical Enterococcus Species

Miankouhi

Malakouti

Mirghafourvand

et al. 2019

Arch Clin Infect Dis

View full text Add to dashboard Cite

show abstract

“…None of the Enterococcus species was positive for gelatin hydrolysis which was similar to the finding of a study done in Egypt by Hashem et al [34]. Manavalan et al reported that 19.84% Enterococci isolates were gelatinase producers which was dissimilar to the finding of this study [20]. Kashef et al also showed that many gelE positive isolates failed to secrete gelatinase in their study [33].…”

Section: Discussionsupporting

confidence: 82%

“…In present study, about 24 (27.6%) hemolysin producing Enterococci was observed. Similarly, 31.61%, 18.25%, 15% hemolysin producing Enterococci were reported by other study [30,20,32]. Kashef et al reported 58% hemolysin producing Enterococci which was quite higher than this study [33].…”

Section: Discussionsupporting

confidence: 76%

Phenotypic and genotypic detection of the virulence factors and their association with antibiotic resistance inEnterococcusspecies

Momotaz¹,

Afroz

Chowdhury³

et al. 2023

Preprint

View full text Add to dashboard Cite

Along with the emergence of drug resistant Enterococcal infection, role of various virulence factors in Enterococci is an emerging concept. A number of virulence factors like biofilm formation, hemolysin production, gelatin hydrolysis have important role in the pathogenesis of Enterococci and also associated with antibiotic resistance. The aim of our study was to detect the virulence factors and their encoding genes (asa, gelE, esp, ebpR, hyl gene for biofilm; cylA gene for hemolysis; gelE gene for gelatin hydrolysis) and also observe their association with antimicrobial resistance Enterococci. A total of 87 Enterococci were collected from different clinical samples. Virulence factors were detected phenotypically and antibiotic sensivity were done by Kirby Bauer disc diffusion method. Virulence genes were detected by conventional multiplex PCR and only the ebpR gene was detected by single conventional PCR. Majority of the isolated Enterococci were E. faecalis (75%) followed by E. faecium (23%) and (2%) E. raffinosus were also detected. About 52.3% of E. faecalis and 35% of E. faecium isolates were biofilm producers. Significant association was found between biofilm formation and asa, esp, ebpR genes both in E. faecalis and in E. faecium. Hemolysis was observed phenotypically in 30.8% isolates of E. faecalis and 20% isolates of E. faecium. Significant association was observed between cylA gene and hemolysin production in E. faecalis. Antibiotic resistance were higher in biofilm and hemolysin producing isolates of both species. Resistance to some antibiotics including ampicillin, ciprofloxacin, gentamicin were significantly higher among biofilm and hemolysin producer in E. faecalis.

show abstract

“…3 Enterococci are intrinsically resistant to antibiotics due to the acquisition of genetic sequences responsible for drug resistance in other bacteria by transferring plasmids, transposons, or chromosome mutations. [4][5][6] In addition to studies analyzing the trend of increased drug resistance in these organisms, several virulence factors have been studied. A few hemolysins, gelatinase, can form biofilms (enterococcal surface proteins).…”

Section: Introductionmentioning

confidence: 99%

Virulence Factors of Clinical and Fecal Isolates of Enterococci Species

Kumar¹,

Rao²,

Natarajan³

et al. 2023

J. Pure Appl. Microbiol.

View full text Add to dashboard Cite

Enterococci species are known commensals of the gastrointestinal flora; however, in recent years, they have emerged as important nosocomial pathogens that possess many virulence factors that are attributed to the pathogenesis of diseases caused by them. The study evaluated and compared the virulence factors of Enterococci isolated from fecal and clinical samples. From the obtained isolates, the clinical enterococcal isolates produced 35%, 20%, and 50%, and fecal isolates produced 23%, 13%, and 13% gelatinase, hemolysin, and biofilm, respectively. Biofilm production determined by the Congo Red agar, tube, and microtiter plate methods was 23%, 39%, and 49%, respectively. The sensitivity of the Congo Red agar and tube method compared to the microtiter plate method was 27% and 46%, respectively, whereas the specificity of both tests was 79%. This study showed that biofilm production plays a significant role in the pathogenesis of diseases caused by Enterococci. Detection of biofilm production using the microtiter plate method is more sensitive and specific than the Congo Red agar and tube method.

show abstract

Phenotypic speciation of enterococci with special reference to prevalence, virulence and antimicrobial resistance

Cited by 7 publications

References 17 publications

Loop-Mediated Isothermal Amplification Assay for Identification of Clinical Enterococcus Species

Loop-Mediated Isothermal Amplification Assay for Identification of Clinical Enterococcus Species

Phenotypic and genotypic detection of the virulence factors and their association with antibiotic resistance inEnterococcusspecies

Virulence Factors of Clinical and Fecal Isolates of Enterococci Species

Contact Info

Product

Resources

About