Background: COVID-19 pandemic becomes a great threat due to continuous rise of the global incidence and emergence of multiple waves of infections in many countries of the world. The diversity of this infection varies from country to country and knowledge regarding demographic characteristics of this infection is essential to combat the pandemic situation. Objective: The aim of this study was to characterize the demographic profile of rRT-PCR (real time reverse transcriptase-polymerase chain reaction) confirmed COVID-19 cases and to provide a timeline regarding rates of infection. Materials and methods: This cross- sectional study was conducted at the Department of Virology of Sir Salimullah Medical College, Dhaka, Bangladesh from May, 2020 to April, 2021. rRT-PCR test was performed to detect SARS-COV2 in 35001 clinical samples and their demographic characteristics were analyzed. Results: Out of 35001 suspected cases,5008 (14.3%) were tested positive of which 58.0% belongs to the age group between 18–45 years.The majority of the cases were male (69.0%). Most of the positive cases became negative (85.0%) within three weeks of infection by rRTPCR test. The highest percentage (29.2%) of confirmed COVID-19 cases was reported in the month of May 2020, then gradual decrease in the subsequent months followed by a sharp rise to 27.2% in the month of March, 2021. Conclusion: This study shows an overall 14.3% positivity among suspected COVID-19 cases where adult males between 18-45 years are more commonly suffered. However, most of the infected persons become rRT-PCR negative within three weeks’ time. A second surge of SARS-COV2 infection has been documented in this study that coincided with second wave of COVID-19 in Bangladesh. Sir Salimullah Med Coll J 2022; 30: 9-13

Along with the emergence of drug resistant Enterococcal infection, role of various virulence factors in Enterococci is an emerging concept. A number of virulence factors like biofilm formation, hemolysin production, gelatin hydrolysis have important role in the pathogenesis of Enterococci and also associated with antibiotic resistance. The aim of our study was to detect the virulence factors and their encoding genes (asa, gelE, esp, ebpR, hyl gene for biofilm; cylA gene for hemolysis; gelE gene for gelatin hydrolysis) and also observe their association with antimicrobial resistance Enterococci. A total of 87 Enterococci were collected from different clinical samples. Virulence factors were detected phenotypically and antibiotic sensivity were done by Kirby Bauer disc diffusion method. Virulence genes were detected by conventional multiplex PCR and only the ebpR gene was detected by single conventional PCR. Majority of the isolated Enterococci were E. faecalis (75%) followed by E. faecium (23%) and (2%) E. raffinosus were also detected. About 52.3% of E. faecalis and 35% of E. faecium isolates were biofilm producers. Significant association was found between biofilm formation and asa, esp, ebpR genes both in E. faecalis and in E. faecium. Hemolysis was observed phenotypically in 30.8% isolates of E. faecalis and 20% isolates of E. faecium. Significant association was observed between cylA gene and hemolysin production in E. faecalis. Antibiotic resistance were higher in biofilm and hemolysin producing isolates of both species. Resistance to some antibiotics including ampicillin, ciprofloxacin, gentamicin were significantly higher among biofilm and hemolysin producer in E. faecalis.