Phenotypic variability in Azospirillum brasilense strains Sp7 and Sp245: Association with dormancy and characteristics of the variants

Pogorelova, Anna Yu.; Mulyukin, A. L.; Antonyuk, L. P.; Гальченко, В. Ф.; Эль-Регистан, Г. И.

doi:10.1134/s0026261709050051

Cited by 15 publications

(3 citation statements)

References 13 publications

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“…We took into account (i) the adaptive potential of the variants with respect to nutrients [24] and (ii) the enhanced dissociative activity of germinating dormant forms (DFs), compared to the vegetative bacterial cells [17,25,26]. Therefore, we investigated the MALDI pro files of the variants that developed on various media after plating of the cells from either growing cultures or DF suspensions.…”

Section: Monitoring the Maldi Spectra Of Bacterial Variantsmentioning

confidence: 99%

“…It was established earlier that the variant forming activ ity and the composition of the intrapopulation spec trum vary depending on the properties of DF formed under various conditions and not only on the medium composition used for DF inoculation [19,21,25,27,28]. Therefore, in order to analyze the MALDI spectra of the variants, they were obtained by plating bacterial DF formed under various conditions on a number of media with different composition.…”

Section: Monitoring the Maldi Spectra Of Bacterial Variantsmentioning

confidence: 99%

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Applicability of MALDI mass spectrometry for diagnostics of phase variants in bacterial populations

et al. 2015

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Efficiency of MALDI mass spectrometry for differentiation between phenotypic phase variants (in colony morphology and virulence/avirulence) was investigated for saprotrophic and opportunistically pathogenic bacteria of five genera (Acinetobacter, Arthrobacter, Rhodococcus, Corynebacterium, and Escheri chia). Analysis of MALDI spectra (on the SA and HCCA matrices) included (1) determination of similarity of the protein spectra as a percentage of the common protein peaks to the total amount of proteins, which reflects the phylogenetic relationships of the objects and has been recommended for identification of closely related species; (2) comparison of intensities of the common peaks; and (3) the presence of specific peaks as determinative characteristics of the variants. Under the standard analytical conditions, the similarity between the MALDI profiles was shown to increase in the row: genus-species-strain-variant. Assessment of inten sities of the common peaks was most applicable for differentiation between phase variants, especially in the case of high similarity of their profiles. Phase variants (A. oxydans strain K14) with similar colony morpho types (S, R, M, and S m ) grown on different media (LB agar, TSA, and TGYg) exhibited differences in their protein profiles reflecting the differences in their physiological characteristics. This finding is in agreement with our previous results on screening of the R. opacus with similar colony morphology and different substrate specificity in decomposition of chlorinated phenols. Analysis of MALDI spectra is probably the only efficient method for detection of such variants.

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Section: Monitoring the Maldi Spectra Of Bacterial Variantsmentioning

confidence: 99%

Section: Monitoring the Maldi Spectra Of Bacterial Variantsmentioning

confidence: 99%

Applicability of MALDI mass spectrometry for diagnostics of phase variants in bacterial populations

et al. 2015

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“…Since phase variation is associated with reversible genomic alterations [ 6 ], the appropriate approach was to compare these morphotypic variants with the original strain using a sensitive method to identify intraspecies strains such as BOX, ERIC, REP, and RAPD PCR [ 32 ]. In the study conducted by Pogorelova et al [ 51 ], BOX and ERIC-PCR fingerprinting were used to observe genomic alterations in Azospirillum brasilense (strains Sp7 and SP245), where ERIC-PCR showed identical profiles of the phase variants. In our case, the BOX and ERIC PCR profiles of both morphotypes were identical to the original strain ( Figure 3 ).…”

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confidence: 99%

Effects of Phenotypic Variation on Biological Properties of Endophytic Bacteria Bacillus mojavensis PS17

Diabankana

Validov

Vyshtakalyuk

et al. 2022

Biology

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The use of microorganism-based products in agricultural practices is gaining more interest as an alternative to chemical methods due to their non-toxic bactericidal and fungicidal properties. Various factors influence the efficacy of the microorganisms used as biological control agents in infield conditions as compared to laboratory conditions due to ecological and physiological aspects. Abiotic factors have been shown to trigger phase variations in bacterial microorganisms as a mechanism for adapting to hostile environments. In this study, we investigated the stability of the morphotype and the effects of phenotypic variation on the biological properties of Bacillus mojavensis strain PS17. B. mojavensis PS17 generated two variants (opaque and translucent) that were given the names morphotype I and II, respectively. The partial sequence of the 16S rRNA gene revealed that both morphotypes belonged to B. mojavensis. BOX and ERIC fingerprinting PCR also showed the same DNA profiles in both morphotypes. The characteristics of morphotype I did not differ from the original strain, while morphotype II showed a lower hydrolytic enzyme activity, phytohormone production, and antagonistic ability against phytopathogenic fungi. Both morphotypes demonstrated endophytic ability in tomato plants. A low growth rate of the strain PS17(II) in a minimal medium was observed in comparison to the PS17(I) strain. Furthermore, the capacity for biocontrol of B. mojavensis PS17(II) was not effective in the suppression of root rot disease in the tomato plants caused by Fusarium oxysporum f. sp. radices-lycopersici stain ZUM2407, compared to B. mojavensis PS17(I), whose inhibition was almost 47.9 ± 1.03% effective.

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Surface colonization by Azospirillum brasilense SM in the indole‐3‐acetic acid dependent growth improvement of sorghum

Kochar

Srivastava

2011

J. Basic Microbiol.

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The key to improving plant productivity is successful bacterial-plant interaction in the rhizosphere that can be maintained in the environment. The results presented here confirm Azospirillum brasilense strain SM as a competent plant growth promoting bacterium over mid- and long-term associations with sorghum. This study establishes that plant growth can be directly correlated with the associated bacterium's indole-3-acetic acid (IAA) production capability as IAA over-expressing variants, SMp30 and SMΔi3-6 fared better than the wild type strain. The auxin antagonist, p-chlorophenoxy isobutyric acid confirmed the role of bacterial IAA in plant growth promotion and verified the presence of larger amount of IAA available to the seeds on inoculation with IAA over-expressing mutants. Microscopic analysis identified the bacterial association at root tips, root-shoot junction and elongation zone and their surface colonizing nature. Scanning electron microscopy identified larger number of root hairs and extensive exopolysaccharide covering in comparison to untreated ones. In addition, vibroid-shaped Azospirilla attached by means of fibrillar material were dispersed along the elongation zone. The notable difference with IAA over-expressing variants was enhanced number of root hairs. Thus, the variant strains may be more efficient surface colonizers of the sorghum root and used as superior bio-inoculants for improving plant productivity.

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Phenotypic variability in Azospirillum brasilense strains Sp7 and Sp245: Association with dormancy and characteristics of the variants

Cited by 15 publications

References 13 publications

Applicability of MALDI mass spectrometry for diagnostics of phase variants in bacterial populations

Applicability of MALDI mass spectrometry for diagnostics of phase variants in bacterial populations

Effects of Phenotypic Variation on Biological Properties of Endophytic Bacteria Bacillus mojavensis PS17

Surface colonization by Azospirillum brasilense SM in the indole‐3‐acetic acid dependent growth improvement of sorghum

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