“…In recent years, there are many analytical methods reported to measure bisulfite content. − Among these methods, a fluorescent probe has been recognized as a highly promising tool to detect bisulfite owing to its outstanding advantages, such as real-time application, high sensitivity, simple operation, and low cost. − Particularly, ratiometric fluorescent probes have been widely designed based on the Michael addition reaction, which shows two different fluorescence emissions that set up a built-in correction to eliminate interference from external factors. − However, the majority of them are excited by one photon, which makes their excitation wavelength so short that it inevitably causes self-fluorescence interference and then generates measurement deviation. Although some research groups have constructed two-photon fluorescent probes for bisulfite detection, their emissions were less than 650 nm, which restricted their further application. − Therefore, how to construct a two-photon near-infrared (NIR) fluorescent probe remains a big challenge worthy of further exploration.…”