Phenylacetic Acid Is ISR Determinant Produced by Bacillus fortis IAGS162, Which Involves Extensive Re-modulation in Metabolomics of Tomato to Protect against Fusarium Wilt

Akram, Waheed; Anjum, Tehmina; Ali, Basharat

doi:10.3389/fpls.2016.00498

Cited by 55 publications

(34 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…More recently, PAA from Bacillus fortis IAGS162 was shown to be the elicitor of ISR in tomato against F. oxysporum f.sp. lycopersici (Akram et al 2016). Through metabolomics techniques, the PGPR Pseudomonas aeruginosa PM12 was found to promote ISR in tomato challenged with F. oxysporum via 3-hydroxy-5-methoxy benzene methanol (HMB).…”

Section: Diverse Pgpr Molecules Elicit Plant Defensementioning

confidence: 99%

Defining plant growth promoting rhizobacteria molecular and biochemical networks in beneficial plant-microbe interactions

2018

View full text Add to dashboard Cite

Background Our knowledge of plant beneficial bacteria in the rhizosphere is rapidly expanding due to intense interest in utilizing these types of microbes in agriculture. Laboratory and field studies consistently document the growth, health and protective benefits conferred to plants by applying plant growth promoting rhizobacteria (PGPR). PGPR exert their influence on other species, including plants, in the rhizosphere by producing a wide array of extracellular molecules for communication and defense. Scope The types of PGPR molecular products are characteristically diverse, and the mechanisms by which they are acting on the plant are only beginning to be understood. While plants may contribute to shape their microbiome, it is these bacterial products which induce beneficial responses in plants. PGPR extracellular products can directly stimulate plant genetic and molecular pathways, leading to increases in plant growth and induction of plant resistance and tolerance. This review will discuss known PGPR-derived molecules, and how these products are implicated in inducing plant beneficial outcomes through complex plant response mechanisms. Conclusions In order to move PGPR research to the next level, it will be important to describe and document the genetic and molecular mechanisms employed in these interactions. In this way, we will be able to restructure and harness these mechanisms in a way that allows for broad-based applications in agriculture. A greater depth of understanding of how these PGPR molecules are acting on the plant will allow more effective development of rhizobacterial applications in the field.

show abstract

Section: Diverse Pgpr Molecules Elicit Plant Defensementioning

confidence: 99%

Defining plant growth promoting rhizobacteria molecular and biochemical networks in beneficial plant-microbe interactions

2018

View full text Add to dashboard Cite

show abstract

“…was the only non-siderophore producing isolate, but it was one of the two strains that produced auxins, and was chosen for this reason. Some authors have shown that auxins are related to the induction of systemic resistance (Akram et al 2016, Petti et al 2012. Three strains, N 10.7 (S. odofirera), N 12.34 (S. rubidaea) and N 11.14 (B. enterophyticus) were chosen with only one biochemical trait, because of their capacity to reduce growth of other strains in plate (data not shown), probably due to the production of antibiotics.…”

Section: Discussionmentioning

confidence: 99%

Bioeffectors as biotechnological tools of the innate immunity: signal transduction pathways involved

Martin‐Rivilla

García‐Villaraco

Ramos‐Solano

et al. 2020

Preprint

View full text Add to dashboard Cite

Background Unravel the complex functioning of plant immune system is essential and something in which great effort is being made since its performance is not entirely clear yet. Knowing plant immune system allows strengthening it and therefore developing a more efficient and environmentally friendly agriculture, avoiding the massive use of agrochemicals and making plants the main protagonist in the defense against pathogens.The use of beneficial rhizobacteria (bioeffectors) and its derived metabolic elicitors are biotechnological alternatives in plant immune system elicitation. The present work aimed to check the ability of 25 bacterial strains selected from a group of 175, isolated from the rhizosphere of Nicotiana glauca, to trigger the innate immune system of Arabidopsis thaliana seedlings against the pathogen Pseudomonas syringae DC3000. A study of the signal transduction pathways involved in plant response was made.Results The selected 25 strains were chosen because of their biochemical traits and avoiding phylogenetic redundancy. The 5 strains, of the previous 25, more effective in the prevention of pathogen infection were used to elucidate signal transduction pathways involved in the plant immune response, studying the differential expression of Salicylic acid and Jasmonic acid/Ethylene pathway marker genes. Some strains stimulated the two pathways with no inhibitory effects between them, while others stimulated either one or the other. Metabolic elicitors of two strains, chosen for their taxonomic affiliation and for the results obtained in the differential expression of the genes studied, were extracted using n-hexane, ethyl acetate and n-butanol, and their capacity to mimic bacterial effect to trigger the immune system of the plant was studied. N-hexane and ethyl acetate were the most effective fractions against the pathogen in both strains, achieving similar protection rates although gene expression responses were different from that obtained by the bacteria. Conclusions Beneficial rhizobacteria and its metabolic elicitors have great potential as biotechnological tools since they are able to improve plant immune system through the triggering of either Salicylic acid or Jasmonic acid/Ethylene pathway or both pathways simultaneously. These results open a huge amount of biotechnological possibilities to develop biological products for agriculture in different situations and plant species.

show abstract

“…After 15 days of treatment applications, third true leaves of tomato cultivars were collected for metabolites extraction. Whole metabolite extraction, preparation of the samples and GC-MS analyses were strictly performed as described by Akram et al 23 Chromatograms and mass spectra obtained after GC-MS operation were analyzed through TURBOMASS (Perkin-Elmer). All the biochemicals showing significant changes were enlisted, and a metabolic pathway was plotted.…”

Section: Effect On Plant Metabolismmentioning

confidence: 99%

Benzenedicarboxylic acid upregulates O48814 and Q9FJQ8 for improved nutritional contents of tomato and low risk of fungal attack

et al. 2019

Self Cite

View full text Add to dashboard Cite

BACKGROUND Tomato is an important food item and a cocktail of phytonutrients. In the current study, metabolites from a non‐pathogenic fungal species Penicillium oxalicum have been exploited to obtain nutritionally augmented tomato fruits from the plants to better withstand against Alternaria alternata infection. RESULTS Initially, bioactivity‐guided assay and chromatographic analyses identified the bioactive metabolites of P. oxalicum [benzenedicarboxylic acid (BDA) and benzimidazole]. Then, ≥3 times elevated quantities of vitamins and other nutritional elements (protein, fat, fibers, and carbohydrates) were achieved by the foliar application of BDA. The maximum increase (625.81%) was recorded in riboflavin contents; however, thiamine showed the second highest enhancement (542.86%). Plant metabolites analysis revealed that jasmonic acid contents were boosted 121.53% to significantly enhance guaiacyl lignin defenses along with the reduction in coumarin contents. The protein profile analysis explored three most actively responding protein species toward BDA applications, (i) palmitoyltransferase protein Q9FLM3; (ii) serine/threonine‐protein kinase O48814; and (iii) E3 ubiquitin‐protein ligase Q9FJQ8. The O48814 improved plant defenses; whereas, Q9FJQ8 protein was negatively regulating cysteine‐type endopeptidase activity and assisted plant to resist schedule alterations. Tomato cultivar with more active innate metabolism was found to be more responsive toward BDA. Furthermore, the bioactive compounds were enriched by using the two‐step extraction method of ethyl acetate and chloroform, respectively. CONCLUSION Penicillium oxalicum a non‐pathogenic fungal species, produced BDA, induced nutritional contents in tomato and protected it against Alternaria alternata. The current study is the first report on the bioactivity of BDA and benzimidazole concerning the nutritional enhancement and plant defense improvement. © 2019 Society of Chemical Industry

show abstract

Phenylacetic Acid Is ISR Determinant Produced by Bacillus fortis IAGS162, Which Involves Extensive Re-modulation in Metabolomics of Tomato to Protect against Fusarium Wilt

Cited by 55 publications

References 65 publications

Defining plant growth promoting rhizobacteria molecular and biochemical networks in beneficial plant-microbe interactions

Defining plant growth promoting rhizobacteria molecular and biochemical networks in beneficial plant-microbe interactions

Bioeffectors as biotechnological tools of the innate immunity: signal transduction pathways involved

Benzenedicarboxylic acid upregulates O48814 and Q9FJQ8 for improved nutritional contents of tomato and low risk of fungal attack

Contact Info

Product

Resources

About