Phenylalanine enhances Quercetin content in In vitro cultures of Abutilon indicum L.

Sajjalaguddam, Ramgopal Rao; Paladugu, Anitha Paladugu

doi:10.7324/japs.2015.501014

Cited by 15 publications

(9 citation statements)

References 4 publications

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“…*The added values present the increase in total contents of phenolic acids in comparison to control cultures with higher concentration of precursor-75 mg/100 ml of phenylalanine. In this experiments phenylalanine was tested in the same concentrations like in above-mentioned investigations (25; 50; 75 and 100 mg/100 ml) (Sajjalaguddam and Paladugu 2015). Results of these two teams indicated, that the best concentration of precursor should be tested experimentally for each in vitro culture.…”

Section: Discussionmentioning

confidence: 83%

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The effect of feeding culture media with biogenetic precursors on high production of depsides in agitated shoot cultures of black and red aronias

Szopa

Kubica

Komsta

et al. 2020

Plant Cell Tiss Organ Cult

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Agitated shoot cultures of two aronias, Aronia melanocarpa (Michx.) Elliott and Aronia arbutifolia (L.) Pers., were maintained on Murashige & Skoog medium (1 mg/l BA and 1 mg/l NAA), both with and without the addition of various biosynthetic precursors of phenolic acids and depsides (phenylalanine, cinnamic acid, benzoic acid and caffeic acid). Each substance was added in 5 concentrations (0.1–10 mmol/l), each concentration at two time points (at the beginning and on the 10th day of cultures). Twenty-four phenolic acids were determined in methanolic extracts of the biomasses collected after 20 days of growth cycles by means of HPLC method with DAD detection. The presence of seven compounds was confirmed in all the extracts—five depsides (neochlorogenic, chlorogenic, cryptochlorogenic, isochlorogenic and rosmarinic acids), and syringic and caffeic acids. The main metabolites in A. melanocarpa shoot extracts were isochlorogenic, chlorogenic and neochlorogenic acids (max. 249.88, 450.35, 192.16 mg/100 g DW). The main metabolites in A. arbutifolia shoot extracts were: chlorogenic, isochlorogenic and cryptochlorogenic acids (max. 361.60, 224.5, 526.2 mg/100 g DW). The largest total amounts of the compounds were confirmed in the cultures of both aronias after the addition of cinnamic acid (989.79 and 661.77 mg/100 g DW, respectively) and caffeic acid (854.99 and 1098.46 mg/100 g DW, respectively) at concentrations of 5 mmol/l on 10th day of growth cycles. These maximum amounts were 3.41, 3.42, 2.95 and 5.67 times higher, respectively, than in the control cultures. This is the first report documenting the high production of depsides in shoot cultures of black and red aronias after feeding with their biosynthetic precursors.

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Section: Discussionmentioning

confidence: 83%

“…In other experiments e.g. with Citrullus colocynthis (Chand Meena et al 2014) and Abutilon indicum (Sajjalaguddam and Paladugu 2015) in which also phenylalanine was tested, it was added into the culture media at point "0".…”

Section: Discussionmentioning

confidence: 99%

The effect of feeding culture media with biogenetic precursors on high production of depsides in agitated shoot cultures of black and red aronias

Szopa

Kubica

Komsta

et al. 2020

Plant Cell Tiss Organ Cult

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“…However, the best time of supplementation with precursors may depend on the tested plant species grown in vitro, e.g., in a Cistanche deserticola culture, the best results of phenylethanoid glycoside production had been obtained after supplementation with Phe on day 8 of culture growth [ 28 ]. In other experiments, e.g., with Citrullus colocynthis [ 29 ] and Abutilon indicum [ 30 ], in which Phe was also tested, the results were best when the culture media were supplemented with Phe on day 0.…”

Section: Resultsmentioning

confidence: 99%

Precursor-Boosted Production of Metabolites in Nasturtium officinale Microshoots Grown in Plantform Bioreactors, and Antioxidant and Antimicrobial Activities of Biomass Extracts

et al. 2021

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The study demonstrated the effects of precursor feeding on the production of glucosinolates (GSLs), flavonoids, polyphenols, saccharides, and photosynthetic pigments in Nasturtium officinale microshoot cultures grown in Plantform bioreactors. It also evaluated the antioxidant and antimicrobial activities of extracts. L-phenylalanine (Phe) and L-tryptophan (Trp) as precursors were tested at 0.05, 0.1, 0.5, 1.0, and 3.0 mM. They were added at the beginning (day 0) or on day 10 of the culture. Microshoots were harvested after 20 days. Microshoots treated with 3.0 mM Phe (day 0) had the highest total GSL content (269.20 mg/100 g DW). The qualitative and quantitative profiles of the GSLs (UHPLC-DAD-MS/MS) were influenced by precursor feeding. Phe at 3.0 mM stimulated the best production of 4-methoxyglucobrassicin (149.99 mg/100 g DW) and gluconasturtiin (36.17 mg/100 g DW). Total flavonoids increased to a maximum of 1364.38 mg/100 g DW with 3.0 mM Phe (day 0), and polyphenols to a maximum of 1062.76 mg/100 g DW with 3.0 mM Trp (day 0). The precursors also increased the amounts of p-coumaric and ferulic acids, and rutoside, and generally increased the production of active photosynthetic pigments. Antioxidant potential increased the most with 0.1 mM Phe (day 0) (CUPRAC, FRAP), and with 0.5 mM Trp (day 10) (DPPH). The extracts of microshoots treated with 3.0 mM Phe (day 0) showed the most promising bacteriostatic activity against microaerobic Gram-positive acne strains (MIC 250–500 µg/mL, 20–21 mm inhibition zones). No extract was cytotoxic to normal human fibroblasts over the tested concentration range (up to 250 μg/mL).

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“…Phenylalanine, as one of the known precursors of flavonoids, when supplemented into the MS medium, it is expected to produce elevated level of target compounds (Shinde et al, 2009). Its application as a precursor for flavonoids production was demonstrated in a large number of plants, such as Balanites aegyptiaca (Bedawat, 2006), Ailanthus excelsa (Rao, 2007), Cocculus pendulus and Tinospora cordifolia (Yadav, 2010), Maytenus emarginata (Mathur and Goswami, 2014),Citrullus colocynthis (Meena et al, 2014) and Abutilon indicum (Sajjalaguddam and Paladugu, 2015). These results indicate that in vitro culture both promote and increase the production of rutin and to a certain extent, quercitrin compounds in callus of C. sempervirens.…”

Section: Effect Of Ga 3 and Phenylalanine On Callus Biomass And Flavomentioning

confidence: 99%

In vitro production of some secondary metabolites from Cupressus sempervirens.

Alhady

El-Fadl

Hegazi

et al. 2020

Journal of advanced Biomedical and Pharmaceutical Sciences

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Cupressus sempervirens L. has known medicinal activities, which are related to the presence of miscellaneous secondary metabolites, involved in patents for pharmaceuticals and/or cosmetics in the market. The aim of this study is to perform a comparative investigation of the main metabolites in the wild, cultivated and callus cultures of C. sempervirens using different treatments and the in vitro production of certain metabolites from C. sempervirens callus cultures in concentrations adequate for use commercially and also to develop a convenient chromatographic method for both the qualitative and quantitative determination of the most beneficial flavonoids (rutin and quercitrin), along with other metabolites (ferruginol, 2-furancarboxaldehyde,5 methyl, pentadecanoic acid, totarol, quinic acid and hinokiol) using HPLC/DAD and GC/MS, respectively. Leaf extract of the wild plant induced reduction in cell viability of some tested cell lines as: human hepatocellular carcinoma cell (HEPG2), lung carcinoma cell (A549), human Caucasian breast adenocarcinoma (MCF7) and especially on human colon cancer cell (HCT116), which was higher than doxorubicin used as a standard. This study is a stepping stone for obtaining natural and renewable bioactive secondary metabolites from C. sempervirens through in vitro callus cultures.

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Phenylalanine enhances Quercetin content in In vitro cultures of Abutilon indicum L.

Cited by 15 publications

References 4 publications

The effect of feeding culture media with biogenetic precursors on high production of depsides in agitated shoot cultures of black and red aronias

The effect of feeding culture media with biogenetic precursors on high production of depsides in agitated shoot cultures of black and red aronias

Precursor-Boosted Production of Metabolites in Nasturtium officinale Microshoots Grown in Plantform Bioreactors, and Antioxidant and Antimicrobial Activities of Biomass Extracts

In vitro production of some secondary metabolites from Cupressus sempervirens.

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