Pheromone-induced polarization is dependent on the Fus3p MAPK acting through the formin Bni1p

Matheos, Dina P.; Metodiev, Metodi V.; Muller, Eric M.; Stone, David E.; Rose, Mark D.

doi:10.1083/jcb.200309089

Cited by 108 publications

(124 citation statements)

References 48 publications

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“…Recruitment of Ssk2p to the vicinity of the polarisome suggests that its components are potential targets for phosphorylation by the kinase. Indeed, both Bni1p and Bud6p have been shown to be phosphorylated in vivo (Goehring et al 2003;Matheos et al 2004;Moseley and Goode 2005). Deletion of BUD6 did not affect bud emergence after a-factor synchronization but had a significant effect on Ssk2p-mediated actin recovery after osmotic stress.…”

Section: Discussionmentioning

confidence: 99%

Requirement for the Polarisome and Formin Function in Ssk2p-Mediated Actin Recovery From Osmotic Stress in Saccharomyces cerevisiae

2007

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Osmotic stress induces activation of an adaptive mitogen-activated protein kinase pathway in concert with disassembly of the actin cytoskeleton by a mechanism that is not understood. We have previously shown that the conserved actin-interacting MAP kinase kinase kinase Ssk2p/MEKK4, a member of the high-osmolarity glycerol (HOG) MAPK pathway of Saccharomyces cerevisiae, mediates recovery of the actin cytoskeleton following osmotic stress. In this study, we have employed in vitro kinase assays to show that Ssk2p kinase activity is activated for the actin recovery pathway via a noncanonical, Ssk1p-independent mechanism. Our work also shows that Ssk2p requires the polarisome proteins Bud6p and Pea2p to promote efficient, polarized actin reassembly but that this requirement can be bypassed by overexpression of Ssk2p. Formin (BNI1 or BNR1) and tropomyosin functions are also required for actin recovery but, unlike for Bud6p and Pea2p, these requirements cannot be bypassed by overexpression of Ssk2p. These results suggest that Ssk2p acts downstream of Bud6p and Pea2p and upstream of tropomyosin to drive actin recovery, possibly by upregulating the actin nucleation activity of the formins.

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Section: Discussionmentioning

confidence: 99%

Requirement for the Polarisome and Formin Function in Ssk2p-Mediated Actin Recovery From Osmotic Stress in Saccharomyces cerevisiae

2007

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“…1) What kinase(s) phosphorylate the carboxyl-terminal half of Bni1? Ste20 and Fus3 kinases are implicated in Bni1 phosphorylation (46,47) and thus represent potential candidates, but many others are possible. 2) What function(s) of Bni1 are regulated by its phosphorylation?…”

Section: Bud6 Stimulates Actin Assembly Nucleated By Bni1 But Notmentioning

confidence: 99%

Differential Activities and Regulation of Saccharomyces cerevisiae Formin Proteins Bni1 and Bnr1 by Bud6

Moseley

Goode

2005

Journal of Biological Chemistry

143

194

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“…In a separate approach, cells were stained by FITC-ConA and TRITC-ConA based on published protocols (Matheos et al 2004;Gao and Bretscher 2009) with the following modifications. Cells were grown in YEPlowD for 16 hr.…”

Section: Budding Pattern Analysismentioning

confidence: 99%

Global Regulation of a Differentiation MAPK Pathway in Yeast

Chavel

Caccamise

Li³

et al. 2014

Genetics

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Cell differentiation requires different pathways to act in concert to produce a specialized cell type. The budding yeast Saccharomyces cerevisiae undergoes filamentous growth in response to nutrient limitation. Differentiation to the filamentous cell type requires multiple signaling pathways, including a mitogen-activated protein kinase (MAPK) pathway. To identify new regulators of the filamentous growth MAPK pathway, a genetic screen was performed with a collection of 4072 nonessential deletion mutants constructed in the filamentous (S1278b) strain background. The screen, in combination with directed gene-deletion analysis, uncovered 97 new regulators of the filamentous growth MAPK pathway comprising 40% of the major regulators of filamentous growth. Functional classification extended known connections to the pathway and identified new connections. One function for the extensive regulatory network was to adjust the activity of the filamentous growth MAPK pathway to the activity of other pathways that regulate the response. In support of this idea, an unregulated filamentous growth MAPK pathway led to an uncoordinated response. Many of the pathways that regulate filamentous growth also regulated each other's targets, which brings to light an integrated signaling network that regulates the differentiation response. The regulatory network characterized here provides a template for understanding MAPK-dependent differentiation that may extend to other systems, including fungal pathogens and metazoans.

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Pheromone-induced polarization is dependent on the Fus3p MAPK acting through the formin Bni1p

Cited by 108 publications

References 48 publications

Requirement for the Polarisome and Formin Function in Ssk2p-Mediated Actin Recovery From Osmotic Stress in Saccharomyces cerevisiae

Requirement for the Polarisome and Formin Function in Ssk2p-Mediated Actin Recovery From Osmotic Stress in Saccharomyces cerevisiae

Differential Activities and Regulation of Saccharomyces cerevisiae Formin Proteins Bni1 and Bnr1 by Bud6

Global Regulation of a Differentiation MAPK Pathway in Yeast

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