Phoma sp. FOM-8108, a producer of gentisylquinones, isolated from sea sand

Abstract

A fungal strain, FOM-8108, that was isolated from sea sand was found to produce gentisylquinone and chlorogentisylquinone, inhibitors of neutral sphingomyelinase. The fungus grew well under normal conditions, in the darkness, or on various agar media, but typical morphological characteristics were not observed to determine its taxonomy. Therefore, culture conditions were studied extensively, resulting in formation of a number of pycnidia by the fungus on the media containing seawater or on natural substrates such as hydrangea leaves, gardenia leaves, and rice straw under natural light or near-ultraviolet radiation exposure. Eventually, from the morphological characteristics of pycnidia, conidiogenous cells, and conidia, strain FOM-8108 was considered to belong to the genus Phoma. Culture studies showed seawater in the fermentation medium was necessary for the strain to produce gentisylquinones. Particularly, a full production of chlorogentisylquinone, which has a chloride ion in the structure, was performed in the medium with higher concentration (75%-100%) of seawater.

Spylidone, a Novel Inhibitor of Lipid Droplet Accumulation in Mouse Macrophages Produced by Phoma sp. FKI-1840

“…5. Furthermore, 13 C-1 H long range couplings of 2 J and 3 J were measured in the 13 C-1 H HMBC spectrum (Fig. 6).…”

“…Color names and hue numbers were determined according to the Color Harmony Manual [12]. For light microscope observation, the pycnidia were fixed by immersion in 8% formaldehyde, frozen in TissueTek O.T.C compound (Sakura), and stored at Ϫ20°C until sectioning [13].…”

“…In the control assay (no drug), about 40% of exogenously added [ 14 C]oleic acid was incorporated into the neutral lipids, [ 14 C]CE (about 25%) and [ 14 C]TG (about 5%), which are the main constituents of lipid droplets in macrophages [13]. As shown in Fig.…”

A new species of Dinemasporium from sugar cane on Irabujima island, Japan

“…The authentic strains for D. strigosum ATCC 200690, D. strigosum MAFF237988, and D. strigosulum MFC-3J601 were obtained from the American Type Culture Collection (ATCC) (Rockville, VA, USA), MAFF (Ibaraki, Japan), and Shionogi Microbiology & Culture Collection (Osaka, Japan), respectively. These strains were cultured on rice straws (Oryza sativa L.) according to the agar-leaf disk method (Kishi 1995) under near-ultraviolet radiation (Yamaguchi et al 2002). The pycnidia produced on the rice straw were sectioned using a Cryostat (Leica, Nussloch, Germany) according to the method described by Yamaguchi et al (2002).…”

“…These strains were cultured on rice straws (Oryza sativa L.) according to the agar-leaf disk method (Kishi 1995) under near-ultraviolet radiation (Yamaguchi et al 2002). The pycnidia produced on the rice straw were sectioned using a Cryostat (Leica, Nussloch, Germany) according to the method described by Yamaguchi et al (2002). Morphological observations were carried out under a light microscope (Vanox-S AH-2; Olympus, Tokyo, Japan) from specimens mounted in a drop of Shear's mounting medium, and a scanning electron microscope (SEM; JSM 5600, JEOL, Tokyo, Japan) from specimens fixed with the vapor from crystalline OsO 4 and coated with gold using a JFC-1200 Fine Coater (JEOL).…”