Phosphatase activity of non‐heme chloroperoxidase from the bacterium <i>Serratia marcescens</i>

Preobrazhenskaya, Yuliya V.; Voskoboev, A I; Burd, V. N.

doi:10.1016/s0014-5793(03)00008-5

Cited by 9 publications

(5 citation statements)

References 15 publications

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“…Acid phosphatase activity was concentrated after filtering through 10 000 Da. Preobrazhenskaya et al (2003) have reported phosphatases in Serratia sp., with a molecular weight of 29 000 Da, whereas Liu et al (2016); Pramanik et al (2018a,b); Bheri and Pandey (2019) reported phosphatases in Pseudomonas spp. with a molecular weight of about 50 000 Da, these molecule sizes are in agreement with our findings.…”

Section: Phosphorus Solubilizationmentioning

confidence: 99%

Bioinoculant production composed by Pseudomonas sp., Serratia sp., and Kosakonia sp., preliminary effect on Allium cepa L., growth at plot scale

et al. 2021

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Phosphorus (P) is an essential nutrient for plant’s development, and its deficiency restricts crop yield. To meet P requirements in agricultural settings, a low-cost culture medium (MT11B) was designed in which a bioinoculant was produced consisting of three bacterial isolates capable of solubilizing P from phosphoric rock (PR). Pseudomonas sp., Serratia sp., and Kosakonia sp. exhibited P solubilization in SMRS1 agar modified with PR (5.0 g L-1), as source of inorganic P. Sowings by isolation were made of the three bacteria on DNAse- and Blood-agar to rule out pathogenicity. At the interaction tests, no inhibition halos were observed; demonstrating there was no antagonism among them, thus they were used to constitute a consortium. Growth curve (12 h) in MT11B demonstrated consortium grew in presence of PR, brewer’s yeast hydrolysate, and glucose at concentrations (2.5 g L-1) fourfold lower than those in SMRS1 (10.0 g L-1); obtaining phosphate solubilizing bacteria of (10.60 ± 0.08/ log10 CFUmL-1 and, at 6 h of culture, acid and alkaline phosphatase enzyme volumetric activities of 2.3 ± 0.8 and (3.80 ± 0.13) UP, respectively. The consortium, releasing phosphorus at a rate of (45.80 ± 5.17) mg L-1 at 6 h of production, was evaluated as bioinoculant in onion plots for five months. Plants receiving a treatment that included 500 mL (10 x 107 CFU mL-1) of bioinoculant plus 100 kg ha-1 of an organic mineral fertilizer exhibited the highest determined response variables (170.1 ± 22.2) mm bulb height, (49.4 ± 6.5) mm bulb diameter, (9.0 ± 1.8) g bulb dry weight, and 15.21 mg bulb-1 total phosphorus (p < 0.05).

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Section: Phosphorus Solubilizationmentioning

confidence: 99%

Bioinoculant production composed by Pseudomonas sp., Serratia sp., and Kosakonia sp., preliminary effect on Allium cepa L., growth at plot scale

et al. 2021

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“…(actinomycetes) . This type of microorganism is also reputed to possess relatively nonspecific BPO and more specific CPO activity, as in the case of Streptomyces lividans , Serrata marcescens , and Pseudomonas pyrrocinia . This type of microorganism may, therefore, also represent a source of TCP contamination in wood.…”

Section: Resultsmentioning

confidence: 99%

Identification of a New Source of Contamination of Quercus sp. Oak Wood by 2,4,6-Trichloroanisole and Its Impact on the Contamination of Barrel-Aged Wines

Chatonnet

Fleury

Boutou

2010

J. Agric. Food Chem.

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Thanks to practical experience in various wineries in recent years, it is now clear that, similarly to the well-known phenomenon in corks, there are several sources of unpredictable contamination of oak wood by 2,4,6-trichloroanisole (TCA). TCA affects staves in the same barrel very sporadically, with extremely limited contaminated areas on the surface that may reach several millimeters in depth. The precise origin of the TCP and TCA in oak wood is not known at this stage. Available data indicate that the phase where stavewood is naturally dried and seasoned is the source of these undesirable organochlorine contaminants. The strictly chemical formation of 2,4,6-trichlorophenol (TCP), derived from organochlorine biocides, was demonstrated to be impossible under traditional cooperage conditions, and its accumulation remained highly improbable. Similarly to previous discoveries in corks, all the analyses of oak wood suggested that the TCP was of biochemical origin. The capacity to biomethylate chlorophenols is well-known and relatively widespread among the usual microflora in stavewood, but the precise origin of the intermediary leading to TCP formation is still unknown. One probable hypothesis is that this reaction involves chloroperoxidase (CPO). Several ideas have been proposed, but the microorganisms responsible for the formation of the TCA precursor in oak wood have not yet been identified. The extent of this problem is still severely underestimated by coopers and barrel-users, due to the extremely unpredictable, localized contamination of the staves.

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“…Peroxidative activity is not the basis for this enhanced resistance, indicating that its activity as a hydrolase on an as yet unidentified plant compound may trigger this phenomenon [37,38]. Recently, the activity spectrum of NHH enzymes was further expanded to include phosphoester hydrolysis [39]. Characterization of the mechanism generating antifungal activity in transgenic plants may thus assist in elucidating the biological function(s) of these enigmatic enzymes and further exploitation of their potential as biocatalysts.…”

Section: Discussionmentioning

confidence: 99%

The thiocarbamate-inducibleRhodococcusenzyme ThcF as a member of the family of Î±/Î² hydrolases with haloperoxidative side activity

Mot

Schrijver

Schoofs

et al. 2003

FEMS Microbiology Letters

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Purified thiocarbamate-inducible ThcF of Rhodococcus erythropolis NI86/21, overexpressed in Escherichia coli, displayed several characteristics of the HASH family of enzymes that groups prokaryotic proteins of the K/L hydrolase superfamily possessing serinedependent hydrolase and/or haloperoxidase activity. Kinetic analysis of bromination and ester hydrolysis revealed a low affinity of ThcF for model substrates. Sulfoxidation of thiocarbamates was demonstrated but probably represents a side activity due to peroxoacid generation by the enzyme. The thcF-linked thcG gene, encoding a LAL-type regulator, triggers expression of thcF in Rhodococcus. The tandem gene organization thcG-thcF is conserved in the thiocarbamate-degrading strain Rhodococcus sp. B30. It is proposed that HASH enzymes may be involved in the metabolism of plant-derived compounds. ß

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Phosphatase activity of non‐heme chloroperoxidase from the bacterium Serratia marcescens

Cited by 9 publications

References 15 publications

Bioinoculant production composed by Pseudomonas sp., Serratia sp., and Kosakonia sp., preliminary effect on Allium cepa L., growth at plot scale

Bioinoculant production composed by Pseudomonas sp., Serratia sp., and Kosakonia sp., preliminary effect on Allium cepa L., growth at plot scale

Identification of a New Source of Contamination of Quercus sp. Oak Wood by 2,4,6-Trichloroanisole and Its Impact on the Contamination of Barrel-Aged Wines

The thiocarbamate-inducibleRhodococcusenzyme ThcF as a member of the family of Î±/Î² hydrolases with haloperoxidative side activity

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