Phosphate distribution and sources in the waters of Huangbai River, China: using oxygen isotope composition of phosphate as a tracer

Wei, Kai; Zeng, Xiongwei; Wang, Chuanshang; Peng, Zhongqin; Wang, Jianpo; Zhou, Fengxia; Chen, Fajin

doi:10.1007/s11356-021-12808-x

Cited by 17 publications

(9 citation statements)

References 25 publications

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“…Among them, the range of δ 18 O P values (6.0− 20.9‰) was the largest in the Huangbai River. 29 The δ 18 O P values were 12.0−20.0‰ (mean of 15.8 ± 3.0‰) in the Mianyuan River, 30 which were higher than those in the Tuojiang River. This was due to the higher δ 18 O P values of the P source in the Huangbai River and Mianyuan River.…”

Section: Resultsmentioning

confidence: 89%

“…The SRP was mainly attributed to fertilizer (49.2 ± 23.2%) and sewage (50.8 ± 23.2%) in the residential/agricultural area. 29 The SRP was mainly attributed to IS, farmland soil, and DS in the upper, middle, and lower reaches of the Mianyuan River, 30 respectively. Our results were consistent with those in the Mianyuan River basin.…”

Section: Major Sources Of Srp Based On Multiple Stablementioning

confidence: 97%

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Using a Multi-isotope Approach and Isotope Mixing Models to Trace and Quantify Phosphorus Sources in the Tuojiang River, Southwest China

Liu

Zhang

et al. 2023

Environ. Sci. Technol.

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Identifying phosphorus (P) sources is critical for solving eutrophication and controlling P in aquatic environments. Phosphate oxygen isotopes (δ 18 O p ) have been used to trace P sources. However, the application of this method has been greatly restricted due to δ 18 O P values from the potential source having wide and overlapping ranges. In this research, P sources were traced by combining δ 18 O p with multiple stable isotopes of nitrogen (δ 15 N), hydrogen (δD), and dissolved inorganic carbon (δ 13 C). Then, a Bayesian-based Stable Isotope Analysis in R (SIAR) model and IsoSource model were used to estimate the proportional contributions of the potential sources in the Tuojiang River. δ 18 O p was not in equilibrium with ambient water, and statistically significant differences in the δ 18 O p values were found between the potential sources, indicating that δ 18 O p can be used to trace the P sources. δ 15 N, δD, and δ 13 C could assist δ 18 O p in identifying the main sources of P. The SIAR and IsoSource models suggested that industrial and domestic sewage was the largest contributor, followed by phosphate rock and phosphogypsum and agricultural sewage. The uncertainty of the calculation results of the SIAR model was lower than that of the IsoSource model. These findings provide new insights into tracing P sources using multiple stable isotopes in watersheds.

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Section: Resultsmentioning

confidence: 89%

Section: Major Sources Of Srp Based On Multiple Stablementioning

confidence: 97%

Using a Multi-isotope Approach and Isotope Mixing Models to Trace and Quantify Phosphorus Sources in the Tuojiang River, Southwest China

Liu

Zhang

et al. 2023

Environ. Sci. Technol.

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“…Accordingly, δ 18 O PO4 can be used to estimate input of P from sources with unique isotopic signatures to soil or water bodies and the degree of biological enzymatic processes that modulate δ 18 O PO4 . Previous studies using δ 18 O PO4 analysis have successfully highlighted the source of P inputs to rivers, 6,10,11 internal P loads from sediment in lakes, 12 and biologically driven P cycling in soil 13,14 …”

Section: Introductionmentioning

confidence: 99%

“…8 Accordingly, δ 18 O PO4 can be used to estimate input of P from sources with unique isotopic signatures to soil or water bodies and the degree of biological enzymatic processes that modulate δ 18 O PO4 . Previous studies using δ 18 O PO4 analysis have successfully highlighted the source of P inputs to rivers, 6,10,11 internal P loads from sediment in lakes, 12 and biologically driven P cycling in soil. 13,14 The procedure of δ 18 O PO4 analysis involves extracting PO 4 from environmental samples (solid or water samples), concentrating PO 4 using magnesium-induced co-precipitation (MagIC) 15 or coprecipitation with iron (Fe) hydroxide, 16 removing other constituents in solution, precipitating the PO 4 as pure Ag 3 PO 4 , and measuring the δ 18 O PO4 value of Ag 3 PO 4 using an isotope ratio mass spectrometry (IRMS) instrument.…”

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confidence: 99%

A new method for phosphate purification for oxygen isotope ratio analysis in freshwater and soil extracts using solid‐phase extraction with zirconium‐loaded resin

Ishida

Kamiya

Uehara

et al. 2022

Rapid Comm Mass Spectrometry

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Rationale Phosphate (PO4) oxygen isotope (δ18OPO4) analysis is increasingly applied to elucidate phosphorus cycling. Due to its usefulness, analytical methods continue to be developed and improved to increase processing efficiency and applicability to various sample types. A new pretreatment procedure to obtain clean Ag3PO4 using solid‐phase extraction (SPE) with zirconium‐loaded resin (ZrME), which can selectively adsorb PO4, is presented and evaluated here. Methods Our method comprises (1) PO4 concentration, (2) PO4 separation by SPE, (3) cation removal, (4) Cl− removal, and (5) formation of Ag3PO4. The method was tested by comparing the resulting δ18OPO4 of KH2PO4 reagent, soil extracts (NaHCO3, NaOH, and HCl), freshwater, and seawater with data obtained using a conventional pretreatment method. Results PO4 recovery of our method ranged from 79.2% to 97.8% for KH2PO4, soil extracts, and freshwater. Although the recovery rate indicated incomplete desorption of PO4 from the ZrME columns, our method produced high‐purity Ag3PO4 and accurate δ18OPO4 values (i.e., consistent with those obtained using conventional pretreatment methods). However, for seawater, the PO4 recovery was low (1.1%), probably due to the high concentrations of F− and SO42− which interfere with PO4 adsorption on the columns. Experiments indicate that the ZrME columns could be regenerated and used repeatedly at least three times. Conclusions We demonstrated the utility of ZrME for purification of PO4 from freshwater and soil extracts for δ18OPO4 analysis. Multiple samples could be processed in three days using this method, increasing sample throughput and potentially facilitating more widespread use of δ18OPO4 analysis to deepen our understanding of phosphorus cycling in natural environments.

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“…The

δ^{18} O_{{PO}_{4}}

values altered by some primary enzyme‐mediated processes (via pyrophosphatase, phosphomonoesterase, and phosphodiesterase) can be predicted from the empirical equations reported in earlier studies 7–12 . Thus, the isotopic characterization of PO 4 enables us to identify the sources of P loading into ecosystems 5,13–15 and to trace the biological cycle of P 16,17 . Despite its efficacy, the

δ^{18} O_{{PO}_{4}}

has not been widely employed in oligotrophic ecosystems due to technical and methodological problems associated with collecting and concentrating sufficient PO 4 for the

δ^{18} O_{{PO}_{4}}

analysis.…”

Section: Introductionmentioning

confidence: 99%

A new sampling method with zirconium‐loaded resin for phosphate oxygen isotope analysis in oligotrophic freshwater systems

Ishida

Tayasu

Onodera

et al. 2022

Rapid Comm Mass Spectrometry

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Rationale The phosphate oxygen isotope ratio (δ18OPO4) is a useful technique to trace the sources and biogeochemical cycles of phosphorus (P) in aquatic ecosystems. However, δ18OPO4 has not been widely used in oligotrophic freshwater systems due to technical and methodological difficulties in collecting sufficient phosphate (PO4) for the δ18OPO4 analysis, which sometimes requires hundreds of liters of the water sample. In this study, a new approach (PaS‐Zir) was developed for the δ18OPO4 analysis in oligotrophic freshwater systems using zirconium (Zr)‐loaded (ZrIRC) resin, which has a high affinity for PO4. Methods ZrClO2 was added to Amberlite IRC748 to obtain the ZrIRC resin. The adsorption/desorption experiment using KH2PO4 with a known value of δ18OPO4 was conducted to determine the adsorption/desorption properties of the resin and the likelihood of isotopic fractionation. By installing mesh bags filled with the resin, the PaS‐Zir approach was used in two rivers with low PO4 concentrations (0.2 and 5.3 μmol/L). A conventional sampling method was also performed in the study river with a higher PO4 concentration to validate the efficacy of the PaS‐Zir method. Results The adsorption/desorption experiment demonstrated that the ZrIRC resin possessed a sufficient adsorption capacity (153 μmol/resin‐mL) and exhibited little isotopic fractionation during the adsorption/desorption processes. Using the PaS‐Zir method, we were able to collect sufficient PO4 samples for the δ18OPO4 analysis from the rivers within at least 4 days of mesh bag installation. The δ18OPO4 values (14.2‰ ± 0.2‰) obtained using the PaS‐Zir method were comparable to those obtained using the conventional method (14.0‰ ± 0.03‰). Conclusion We proved that the PaS‐Zir method is applicable to oligotrophic freshwater systems and is generally more efficient than the conventional method. In addition, our method is useful for improving the understanding of the P dynamics of oligotrophic ecosystems because of the extremely low concentration of PO4 commonly found in them, which are often prone to P pollution.

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Phosphate distribution and sources in the waters of Huangbai River, China: using oxygen isotope composition of phosphate as a tracer

Cited by 17 publications

References 25 publications

Using a Multi-isotope Approach and Isotope Mixing Models to Trace and Quantify Phosphorus Sources in the Tuojiang River, Southwest China

Using a Multi-isotope Approach and Isotope Mixing Models to Trace and Quantify Phosphorus Sources in the Tuojiang River, Southwest China

A new method for phosphate purification for oxygen isotope ratio analysis in freshwater and soil extracts using solid‐phase extraction with zirconium‐loaded resin

A new sampling method with zirconium‐loaded resin for phosphate oxygen isotope analysis in oligotrophic freshwater systems

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