Phosphate solubilizing rhizobacteria as alternative of chemical fertilizer for growth and yield of Triticum aestivum (Var. Galaxy 2013)

Abstract

AimThe presence of Phosphorus as a macronutrient in soil is necessary for plant growth and its deficiency restricts crop yield. Therefore, the aim of current study is to isolate promising rhizospheric phosphate solubilizing bacteria presenting with plant growth promoting (PGP) traits and their utilization as biofertilizers to improve Triticum aestivum (Var. Galaxy 2013) growth and nutrition.MethodOut of 30 isolates obtained from rhizosphere of various plants of different regions, 10 best PSRB strains (WumS-3, WumS-4, WumS-5, WumS-11, WumS-12, WumS-21, WumS-24, WumS-25, WumS-26 and WumS-28) were selected based on their high P solubilization and good PGP (auxin, psiderphore, HCN, Nitrogen fixation) activities. Triticum aestivum (Var. Galaxy 2013) was used as an experimental crop under laboratory and field conditions.ResultsIn this study, P solubilization capacity of selected strains were found 4–7 solubilization index on agar plate and 30–246 µg/ml in liquid broth respectively. The optimum conditions for phosphate solubilization under in vitro condition were found 35 °C at pH 7, glucose as good carbon source and ammonium nitrate as a good nitrogen source. Furthermore, the selected strains had the ability to produces phytohormones (indole acetic acid), siderophore, ammonia and Hydrogen Cyanide. Finally, PSRB inoculum showed significant (p < 0.05) increase (50%–80%) in seed germination while 10–90% increase in root length and shoot length was found as compared to control in laboratory condition. Under natural conditions, 40–80% increase in seed germination while 5–34.8% increase in shoot length and 5–96% increase in seed weight was also observed.ConclusionIsolated strains are promising PSRB that enhance plant growth and this research is a base for recommending the use of these bacterial strains for biofertilizer, as an alternative of chemical fertilizer, for Triticum aestivum L. production.

Phosphate Solubilizing Rhizobacteria Could Have a Stronger Influence on Wheat Root Traits and Aboveground Physiology Than Rhizosphere P Solubilization

“…For plants inoculated with PSB 5 (exhibiting the highest PSC), the lower rhizosphere available P fraction may partly be attributed to important RDW and root depth that indicate an important root growth presumably responsible for increased root P uptake and P content in wheat shoots (Table 4). In line with these findings, experimental evidences about soil bacterial P bio-solubilization are available (Mamta et al, 2010;Batool and Iqbal, 2019;Emami et al, 2019), meanwhile relationship between PSB and root functional traits and their extension within the rhizosphere are not yet fully deciphered and so more when it comes more particularly to temporal variations throughout crop growth stages. Overall, our findings show for the first time that PSB may exhibit different PSC over growing time (three plant growth stages) and that P solubilization rate at the rhizosphere level likely difficult to estimate while roots continue to grow spatially and temporally.…”

Phytobeneficial and salt stress mitigating efficacy of IAA producing salt tolerant strains in Gossypium hirsutum

“…Plates were incubated at 37 ± 2 °C for 48 h. Salt tolerance was determined in terms of (MIC) of salt. Salt tolerant bacterial strains were further checked for their drought tolerance and PGP traits like IAA, siderophore and HCN production as describe earlier ( Batool and Iqbal, 2019 ).…”

Characterization and Diversity of plant growth promoting Endophytic and Rhizosphere Bacteria isolated from theRhizosphere and tissues of Pepper and their effect on plant growth promotion and disease suppression of Phytophthora capsici

“…60 studied on Phosphate solublization in the National Botanical Research institutes on phosphate growth medium (NBRIP) and found that the concentration of soluble phosphate ranged between 101.91 µg/ml and 174.33 µg/ml. 61 also studied on the solubilization of TCP and found that the soluble phosphate concentration was ranged from 41.20 to 119.95 µg/ml in the NBRIP medium. The better performance of AAUFE29 (Enterobacter sp.V.H.18) to release the available phosphorus in PVK medium indicates the role played by insoluble phosphate sources to trigger microbial action.…”