Phosphatidylcholine is transferred from chemically‐defined liposomes to chloroplasts through proteins of the chloroplast outer envelope membrane

Yin, Congfei; Andersson, Mats X.; Zhang, Hongsheng; Aronsson, Henrik

doi:10.1016/j.febslet.2014.11.044

Cited by 10 publications

(5 citation statements)

References 44 publications

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“…The ER proliferation and the absence of DGTS in chloroplast purified fraction indicates that DGTS is produced in the ER and not transported into the chloroplasts. It confirms that lipid trafficking from the ER toward chloroplast membrane is selective as it was already shown that PC was able to be transferred from liposomes to chloroplast but not PE (Yin et al, 2015). The transfer of DGTS to the rest of the endomembrane system could be achieved by vesicular trafficking but this need to be investigated as well as a potential mitochondria transfer.…”

Section: Discussionsupporting

confidence: 58%

DGTS overproduced in seed plants is excluded from plastid membranes and promotes endomembrane expansion

Salomon,

Schilling,

Albrieux

et al. 2024

Preprint

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Plants and algae must adapt to environmental changes, facing various stresses that negatively impact their growth and development. One common stress is phosphate (Pi) deficiency, which is often in limiting quantity in the environment. In response to Pi deficiency, these organisms increase Pi uptake and remobilize intracellular Pi. Phospholipids are degraded to provide Pi and replaced by non-phosphorous lipids, such as glycolipids or betaine lipids. During the evolution, seed plants lost their capacity to synthesize betaine lipid. By expressing BTA1 genes, involved in the synthesis of diacylglyceryl-N,N,N-trimethyl-homoserine (DGTS), from different species, our work shows that DGTS can be produced in seed plants. In Arabidopsis, expressing BTA1 under a phosphate starvation-inducible promoter resulted in limited DGTS production without having any impact on plant growth or lipid remodeling. In transient expression systems inNicotiana benthamiana, leaves were able to accumulate DGTS up to 20 % of their glycerolipid content at a slight expense of galactolipid and phospholipid production. At the subcellular level, we showed that DGTS is absent from plastid and seems to be enriched in endomembrane, driving an ER membrane proliferation. Finally, DGTS synthesis pathway seems to compete with PC synthesis via the Kennedy pathway but does not seem to be derived from PC diacylglycerol backbone and therefore does not interfere with the eukaryotic pathway involved in galactolipid synthesis.

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Section: Discussionsupporting

confidence: 58%

DGTS overproduced in seed plants is excluded from plastid membranes and promotes endomembrane expansion

Salomon,

Schilling,

Albrieux

et al. 2024

Preprint

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“…Further work confirmed that ER membranes can donate labeled phospholipids to isolated chloroplasts in a time and temperature dependent fashion without any cytosolic factors (Andersson et al 2004;Xu et al 2008;Yin et al 2015). Additionally, protease digestion to remove chloroplast OEM proteins impaired PC uptake, though proteins responsible have yet to be identified (Yin et al 2015). It is also possible that soluble lipid transfer proteins are involved, as soluble proteins were previously observed to increase transfer of PC to the chloroplast (Miquel et al 1988).…”

Section: Lipid Transport To the Chloroplastmentioning

confidence: 68%

“…Subbsequent incubation of the chloroplasts with phospholipase C generated DAG from PC, which allowed synthesis of labeled MGDG (Oursel et al 1987). Further work confirmed that ER membranes can donate labeled phospholipids to isolated chloroplasts in a time and temperature dependent fashion without any cytosolic factors (Andersson et al 2004;Xu et al 2008;Yin et al 2015). Additionally, protease digestion to remove chloroplast OEM proteins impaired PC uptake, though proteins responsible have yet to be identified (Yin et al 2015).…”

Section: Lipid Transport To the Chloroplastmentioning

confidence: 82%

Lipid transport required to make lipids of photosynthetic membranes

2018

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Photosynthetic membranes provide much of the usable energy for life on earth. To produce photosynthetic membrane lipids, multiple transport steps are required, including fatty acid export from the chloroplast stroma to the endoplasmic reticulum, and lipid transport from the endoplasmic reticulum to the chloroplast envelope membranes. Transport of hydrophobic molecules through aqueous space is energetically unfavorable and must be catalyzed by dedicated enzymes, frequently on specialized membrane structures. Here, we review photosynthetic membrane lipid transport to the chloroplast in the context of photosynthetic membrane lipid synthesis. We independently consider the identity of transported lipids, the proteinaceous transport components, and membrane structures which may allow efficient transport. Recent advances in lipid transport of chloroplasts, bacteria, and other systems strongly suggest that lipid transport is achieved by multiple mechanisms which include membrane contact sites with specialized protein machinery. This machinery is likely to include the TGD1, 2, 3 complex with the TGD5 and TGD4/LPTD1 systems, and may also include a number of proteins with domains similar to other membrane contact site lipid-binding proteins. Importantly, the likelihood of membrane contact sites does not preclude lipid transport by other mechanisms including vectorial acylation and vesicle transport. Substantial progress is needed to fully understand all photosynthetic membrane lipid transport processes and how they are integrated.

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“…Isolated plastids are able to uptake PC, but not PE, coming from microsomes or liposomes (i.e., small vesicles mimicking membranes), supporting the transfer of PC molecules from the ER to plastids (Andersson et al, 2004; Yin et al, 2015). In addition, a lipid flippase, ALA10, able to translocate phospholipids from one leaflet of a membrane to another, was reported to stimulate PC export from the ER in Arabidopsis thaliana (Poulsen et al, 2015; Botella et al, 2016).…”

Section: Lipid Trafficking During Plant Developmentmentioning

confidence: 96%

Lipid Trafficking at Membrane Contact Sites During Plant Development and Stress Response

Michaud

Jouhet

2019

Front. Plant Sci.

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The biogenesis of cellular membranes involves an important traffic of lipids from their site of synthesis to their final destination. Lipid transfer can be mediated by vesicular or non-vesicular pathways. The non-vesicular pathway requires the close apposition of two membranes to form a functional platform, called membrane contact sites (MCSs), where lipids are exchanged. These last decades, MCSs have been observed between virtually all organelles and a role in lipid transfer has been demonstrated for some of them. In plants, the lipid composition of membranes is highly dynamic and can be drastically modified in response to environmental changes. This highlights the importance of understanding the mechanisms involved in the regulation of membrane lipid homeostasis in plants. This review summarizes our current knowledge about the non-vesicular transport of lipids at MCSs in plants and its regulation during stress.

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Phosphatidylcholine is transferred from chemically‐defined liposomes to chloroplasts through proteins of the chloroplast outer envelope membrane

Cited by 10 publications

References 44 publications

DGTS overproduced in seed plants is excluded from plastid membranes and promotes endomembrane expansion

DGTS overproduced in seed plants is excluded from plastid membranes and promotes endomembrane expansion

Lipid transport required to make lipids of photosynthetic membranes

Lipid Trafficking at Membrane Contact Sites During Plant Development and Stress Response

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