Phosphatidylcholine nanovesicles coated with chitosan or chondroitin sulfate as novel devices for bacteriocin delivery

Nanosystems as Vehicles for the Delivery of Antimicrobial Peptides (AMPs)

“…For all that, encapsulation of AMPs into liposomes is a desirable strategy to prevent the drawbacks associated with the direct application of these AMPs, since cytotoxicity could be decreased and stability and bioactivity enhanced [85]. Thus, the number of publications researching the antimicrobial activity of AMPs encapsulated in liposomes, in fields such as food technology [86,87,88] and biomedicine [47,85,89], has risen during the last few years (Table 4).…”

“…In previously reported works, Lopes et al (2017) [87] and da Silva et al (2014) [89] encapsulated nisin in phosphatidylcholine liposomes coated with biocompatible polysaccharides such as pectin [87], polygalacturonic acid [87], chitosan [89], or chondroitin sulphate [89] and evaluated their efficiency in inhibiting Listeria innocua 6a, Listeria monocytogenes ATCC 7644, Listeria monocytogenes 4b, Listeria sp. Str1, and Listeria sp.…”

“…In the former study, in vitro release studies for polysaccharide-coated liposomes resulted to be lower when compared with uncoated liposomes [87], evidencing a potential use for food application. In the latter, the formulation containing chitosan was more stable and more efficient for inhibiting Listeria monocytogenes when compared with the chondroitin sulphate-coated liposomes [89]. Also, Pu et al (2016) [88] reported the antibacterial and anti-biofilm properties of chitosancoated liposomes encapsulating Apep10.…”

Preparación y caracterización antimicrobiana de péptidos encapsulados en suportes biopolimericos con una potencial aplicación biotecnológica

“…There are reports using mixtures of lipids such as PC and phosphatidylglycerol to generate cationic or anionic liposomes (P. da Silva Malheiros, Daroit, da Silveira, & Brandelli, 2010, Were et al, 2004. The efficiency of entrapment of nisin in liposomes is reported in different works, for liposomes elaborated from raw lecithin they reported a 92% entrapment (Patrícia da Silva Malheiros, Yasmine Miguel Serafini Micheletto, et al, 2010), for liposomes with PC of commercial grade of 70% of entrapment (Zou et al, 2012), likewise there are reports of liposomes covered with chitosan and chondroitin sulfate with a 67% entrapment (da Silva, Boelter, da Silveira, & Brandelli, 2014 ), liposomes with pectin show entrapments close to 94% (Lopes et al, 2017) In the present work, conventional liposomes and stealth liposomes were elaborated from purified lecithin, exhibiting an average size of 150 nm to 210 nm, which enter the classification of large liposomes; the average Z power was -52 mV to + 24 mV, suggesting stable suspensions; the encapsulation efficiency was 87% to 95%.…”

Evaluation of the Antimicrobial Activity of Cationic Peptides Loaded in Surface-Modified Nanoliposomes against Foodborne Bacteria

“…Additionally, Nisin-loaded pectin or polygalacturonic acid-coated liposomes exhibited an enhanced efficiency against L. innocua 6a when compared with free Nisin [16]. Thus far, there have been few studies examining the antimicrobial effect of AMPs encapsulated into coated liposomes [16,17,18,19] and the current knowledge of how the structural and physicochemical properties of peptide impacts their ability to be encapsulated into liposomes is limited.…”