Phosphatidylglycerol depletion affects photosystem II activity in Synechococcus sp. PCC 7942 cells

Bogos, Balázs; Ughy, Bettina; Domonkos, Ildikó; Laczkó-Dobos, Hajnalka; Komenda, Josef; Abasova, Leyla; Cser, Krisztián; Vass, Imre; Sallai, Anna; Wada, Hajime; Gombos, Zoltán

doi:10.1007/s11120-009-9497-0

Cited by 23 publications

(12 citation statements)

References 43 publications

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“…It indicates that the essential pool of PG is very small. In agreement with previous reports (Gombos et al, 2002;Bogos et al, 2010;Itoh et al, 2012), PG starvation triggered a decrease in Chl content; the loss of Chl per cell was already apparent after 48 h followed by a gradual decrease to approximately 50% of the original Chl level after 5 d ( Fig. 1; Table I).…”

Section: Pg Depletion Results In Inhibition Of De Novo Chl Biosynthessupporting

confidence: 93%

Lack of Phosphatidylglycerol Inhibits Chlorophyll Biosynthesis at Multiple Sites and Limits Chlorophyllide Reutilization in Synechocystis sp. Strain PCC 6803

et al. 2015

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(J.Kom.).The negatively charged lipid phosphatidylglycerol (PG) constitutes up to 10% of total lipids in photosynthetic membranes, and its deprivation in cyanobacteria is accompanied by chlorophyll (Chl) depletion. Indeed, radioactive labeling of the PG-depleted DpgsA mutant of Synechocystis sp. strain PCC 6803, which is not able to synthesize PG, proved the inhibition of Chl biosynthesis caused by restriction on the formation of 5-aminolevulinic acid and protochlorophyllide. Although the mutant accumulated chlorophyllide, the last Chl precursor, we showed that it originated from dephytylation of existing Chl and not from the block in the Chl biosynthesis. The lack of de novo-produced Chl under PG depletion was accompanied by a significantly weakened biosynthesis of both monomeric and trimeric photosystem I (PSI) complexes, although the decrease in cellular content was manifested only for the trimeric form. However, our analysis of DpgsA mutant, which lacked trimeric PSI because of the absence of the PsaL subunit, suggested that the virtual stability of monomeric PSI is a result of disintegration of PSI trimers. Interestingly, the loss of trimeric PSI was accompanied by accumulation of monomeric PSI associated with the newly synthesized CP43 subunit of photosystem II. We conclude that the absence of PG results in the inhibition of Chl biosynthetic pathway, which impairs synthesis of PSI, despite the accumulation of chlorophyllide released from the degraded Chl proteins. Based on the knowledge about the role of PG in prokaryotes, we hypothesize that the synthesis of Chl and PSI complexes are colocated in a membrane microdomain requiring PG for integrity.

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Section: Pg Depletion Results In Inhibition Of De Novo Chl Biosynthessupporting

confidence: 93%

Lack of Phosphatidylglycerol Inhibits Chlorophyll Biosynthesis at Multiple Sites and Limits Chlorophyllide Reutilization in Synechocystis sp. Strain PCC 6803

et al. 2015

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“…PCC 6803 and Synechococcus sp. PCC 7942, a number of mutant studies have demonstrated that PG is physiologically essential and is notably involved in the activity of both photosystems, influencing the dimerization and reactivation of core complexes (Sakurai et al, 2003;Bogos et al, 2010;Yamamoto, 2016). X-ray crystallographic analysis of photosystem II at 1.9 Å resolution has identified 5 PG molecules bound to photosystem II, directly connected to the D1 protein and plastoquinone Q B (Itoh et al, 2012;Mizusawa and Wada, 2012).…”

Section: Cold-induced Desaturations Of Acyl Chains In Synechococcus Smentioning

confidence: 99%

Thermoacclimation and genome adaptation of the membrane lipidome in marine Synechococcus

Pittera

Jouhet

Breton

et al. 2017

Environmental Microbiology

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The marine cyanobacteria of the genus Synechococcus are important primary producers, displaying a wide latitudinal distribution that is underpinned by diversification into temperature ecotypes. The physiological basis underlying these ecotypes is poorly known. In many organisms, regulation of membrane fluidity is crucial for acclimating to variations in temperature. Here, we reveal the detailed composition of the membrane lipidome of the model strain Synechococcus sp. WH7803 and its response to temperature variation. Unlike freshwater strains, membranes are almost devoid of C18, mainly containing C14 and C16 chains with no more than two unsaturations. In response to cold, we observed a rarely observed process of acyl chain shortening that likely induces membrane thinning, along with specific desaturation activities. Both of these mechanisms likely regulate membrane fluidity, facilitating the maintenance of efficient photosynthetic activity. A comprehensive examination of 53 Synechococcus genomes revealed clade-specific gene sets regulating membrane lipids. In particular, the genes encoding desaturase enzymes, which is a key to the temperature stress response, appeared to be temperature ecotype-specific, with some of them originating from lateral transfers. Our study suggests that regulation of membrane fluidity has been among the important adaptation processes for the colonization of different thermal niches by marine Synechococcus.

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“…However, Synechococcus sp. PCC 7942 has been regarded as an obligate photoautotroph (Kratz and Myers 1955;Zhang et al 1998), and many studies have been done in photoautotrophic cultures (e.g., Tsinoremas et al 1994;Sauer et al 2001;Bogos et al 2010). Despite of that, there have been studies which suggest that some obligate photoautotrophic cyanobacteria can assimilate organic carbon sources (Hoare et al 1967;Pelroy et al 1972;Pearce and Carr 1967;Khoja and Whitton 1971).…”

Section: Introductionmentioning

confidence: 99%

Carbon metabolism and energy conversion of Synechococcus sp. PCC 7942 under mixotrophic conditions: comparison with photoautotrophic condition

et al. 2011

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To investigate the carbon metabolism and energy conversion efficiency of the cyanobacterium Synechococcus sp. PCC 7942 under mixotrophic conditions, we studied its growth characteristics in mixotrophic cultures with glucose and with acetate, respectively, and further discussed the carbon metabolism and energy utilization based on metabolic flux analysis. Results showed that both glucose and acetate could enhance the growth of Synechococcus sp. PCC 7942. The metabolic flux through the glycolytic pathway, tricarboxylic acid cycle, and mitochondrial oxidative phosphorylation was affected by the two organic substrates. Additionally, the cellular composition was also modulated by glucose and acetate. Under mixotrophic conditions, glucose exerts more significant impact on the diminishment of photochemical efficiency. Although the contribution of light energy was smaller, the cell yields based on total energy in mixotrophic cultures were higher compared with that of photoautotrophic one. On the basis of chlorophyll fluorescence analysis, the actual energy conversion efficiencies based on ATP synthesis in the photoautotrophic, glucose-mixotrophic, and acetatemixotrophic cultures were evaluated to be 4.59%, 5.86%, and 6.60%, respectively. Keywords Synechococcus sp. PCC 7942 . Mixotrophic cultivation . Carbon metabolism . Energy conversion . Metabolic flux analysis . Chlorophyll fluorescence analysis Nomenclature a ij Stoichiometric coefficient of metabolite i in the jth reaction (−) A m×nMatrix of stoichiometric coefficient (−) A 0 Side area of flask receiving incident light (m 2 ) E abLight energy absorbed by the biomass unit (kJ g −1 h −1 ) E P Actual light energy for photosynthesis (kJ g −1 h −1 ) E C Chemical energy received from organic carbon resources (kJ g −1 h −1 ) E C,ACE Chemical energy originated from acetate (kJ g −1 h −1 ) E C,GLC Chemical energy originated from glucoseThe total absorbed energy (kJ g −1 h −1 ) I 0 Incident light intensity (μmol m− 2 s −1 ) K a Effective absorption coefficient of light (m −1 ) rVector of m-dimensional metabolite accumulation rate (mmol g −1 h −1 ) r ATP Relative rate of ATP synthesized (mmol g −1 h −1 ) r ACE Relative rate of acetate consumption (mmol g −1 h −1 ) r ex Subvector of extracellular metabolite accumulation rate (mmol g −1 h −1 )Relative rate of glucose consumption (mmol g −1 h −1 ) r i Accumulation rate of metabolite i (mmol g −1 h −1 ) r in Subvector of intracellular metabolite accumulation rate (mmol gof cell mass based on ATP generation (g mol-ATP −1 ) Y E Cell yield based on total light energy (g kJ −1 ) ΔG o ACE Free energy change of acetate complete oxidation (kJ mol −1 ) ΔG o ATP Free energy change of ATP hydrolysis (kJ mol −1 ) ΔG o GLC Free energy change of glucose complete oxidation (kJ mol −1 ) μ Specific growth rate (h −1 ) 8 Vector of measurement noise variancecovariance (−) Φ PSII The efficiency of photosystem II photochemistry (−) Ψ ATP The efficiency of energy conversion on ATP synthesis (%)

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Phosphatidylglycerol depletion affects photosystem II activity in Synechococcus sp. PCC 7942 cells

Cited by 23 publications

References 43 publications

Lack of Phosphatidylglycerol Inhibits Chlorophyll Biosynthesis at Multiple Sites and Limits Chlorophyllide Reutilization in Synechocystis sp. Strain PCC 6803

Lack of Phosphatidylglycerol Inhibits Chlorophyll Biosynthesis at Multiple Sites and Limits Chlorophyllide Reutilization in Synechocystis sp. Strain PCC 6803

Thermoacclimation and genome adaptation of the membrane lipidome in marine Synechococcus

Carbon metabolism and energy conversion of Synechococcus sp. PCC 7942 under mixotrophic conditions: comparison with photoautotrophic condition

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