Phosphatidylinositol kinase activity of a plasma membrane‐associated calcium‐activated protein kinase from pea

Blowers, David P.; Trewavas, Anthony

doi:10.1016/0014-5793(88)80231-x

Cited by 12 publications

(4 citation statements)

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“…One species, the reaction intermediate, is usually a phosphohistidyl residue (Munoz-Dorado et al, 1993). The presence of a reactive phosphohistidine may account for the opportunistic phosphotransferase activity as represented by low-protein kinase activity of pp18 from both sources and phosphatidylinositol kinase activity of pea pp18 (Blowers and Trewavas, 1988). The reaction mechanism may also explain the riipid tumover of 32P label in in vitro experiments here and by Trewavas (1987, 1989) as ADP stimulated the tumover of 32Pi in pea pp18.…”

Section: Discussionmentioning

confidence: 64%

Characterization of a Low Molecular Mass Autophosphorylating Protein in Cultured Sugarcane Cells and Its Identification as a Nucleoside Diphosphate Kinase

et al. 1994

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A low molecular m a s (18 kD) phosphoprotein (pp18) was characterized and purified from cultured sugarcane (Saccharum officinarum 1.) cell line H50-7209. Autophosphorylation assays were used to detect pp18 after separation by sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PACE). Only pp18 was detected by a brief in situ phosphorylation method, whereas additional putative protein kinases were detected by an extended method. pp18 was present in both microsomal membrane and soluble fractions and exhibited anomalous turnover of 32P label during in vitro phosphorylation experiments with highest levels present at shorter incubation times. Two major isoforms of the protein were identified in two-dimensional isoelectric focusing/ SDS-PACE of crude extracts and microsomal fractions. The levels of pp18 were enhanced approximately 4-fold by heat shock at 36°C and the elevated pp18 decayed after heat shock was discontinued. pp18 was purified to apparent homogeneity, could be phosphorylated on serine residues, and also exhibited kinase-like activity toward histone H1. l h e amino acid sequence obtained from a cyanogen bromide digest was greater than 80% identical to nucleoside diphosphate (NDP) kinases from a variety of organisms. Biochemical analysis of the purified protein confirmed the identity as NDP kinase. Thus, NDP kinase appears to be modulated by heat shock in plants.Signal transduction events are likely to occur in response to environmental stresses in cells, in particular, the heatshock response. Within minutes after the onset of heat-shock conditions, altered transcription, translation, and metabolism are observed (Lindquist, 1986). These changes may involve well-known signal transduction mechanisms such as alterations in phosphatidylinositol metabolism, in cellular Ca2+ concentration (Calderwood et al., 1988), and in protein phosphorylation (Schlesinger, 1988). In sugarcane (Saccharum officinarum L.) cells, the induction of the events leading to the development of thermotolerance has a temperature optimum of 36 to 38OC with a minimum induction period of about 30

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Section: Discussionmentioning

confidence: 64%

Characterization of a Low Molecular Mass Autophosphorylating Protein in Cultured Sugarcane Cells and Its Identification as a Nucleoside Diphosphate Kinase

et al. 1994

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“…++ como componente estructural de las membranas y paredes celulares, se debe, principalmente, a la capacidad que tiene el elemento para formar complejos estables, pero reversibles con los pectatos de la lamela media, que están conformadas de cadenas de residuos de ácido poligalacturónico con inserciones de ramnosa, de grupos fosfatos y de carboxilatos de las proteínas, manteniendo, de esta manera, la permeabilidad selectiva, la integridad y la compartimentalización celular (Blowers & Trewavas, 1988;Roux & Slocum, 1982;Rahman & Punja, 2007). Debido a que su transporte se realiza, preferiblemente, por las células muertas del xilema, los síntomas visuales de deficiencia, por lo general, son observados en los tejidos jóvenes.…”

Section: La Mayoría De Las Funciones Del Caunclassified

“…Aunque los cambios fisiológicos y bioquímicos de la senes-Bohórques, W.; Gómez, J.; Flórez, V.: Ca y B en el ennegrecimiento de pétalos cencia están controlados genéticamente, existe evidencia que supone la participación del Ca ++ en este proceso. Su función estaría ligada al mantenimiento estructural de algunas moléculas de la membrana celular, a través de uniones con moléculas cargadas negativamente, como los fosfolípidos y las proteínas, que mantienen funcionando a la célula (Blowers & Trewavas, 1988;Roux & Slocum, 1982) La deficiencia de Ca ++ , especialmente en el tejido meristemático, puede reducir la estabilidad de la pared y la membrana celular, conduciendo a la muerte del tejido. Su deficiencia puede ser debida más a un transporte inadecuado por los conductos vasculares de la planta que causada por baja absorción o disponibilidad en el suelo (Cresswell & Weir, 1997).…”

Section: Una Baja Concentración De Caunclassified

Factores nutricionales y alteración en el contenido de antocianinas relacionados con el ennegrecimiento de los pétalos en rosa (Rosa sp.)

Bohórquez

Gómez

Flórez

2013

Rev. U.D.C.A Act. & Div. Cient.

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One of the most important physiological disorders of red rose cultivars is blackening of petals, it affects outer and inner ones and results in flowers of poor quality. The goal of this review is to make an approach to the blackening disorder, determining the main possible causes in order to make available to growers, academics, scientifics as well as stakeholders, the inputs that allows to unveil its origin and to optimize its handling. Symptoms of blackening are showed especially when the flowers have been harvested and stored under cold room conditions; due to high accumulation of pigments, such as anthocyanins, also could be due to stress conditions, such as high or low UV radiation, low temperatures or a nutrient imbalance in the affected tissues.The following subjects will be treated: i) calcium and boron and their interaction, ii) calcium and physiological disorders, iii) pigments involved in the petals red color, iv) role of the phenolic compounds, particularly the anthocyanins, which are responsible for most of the red, pink, purple and blue colors observed in plant parts; as well as the environmental factors that can influence the blackening on red rose.

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“…The M, 18,000 component was previously identified as the catalytic subunit (3). Additionally, a copurifying lipid, thought to be lysophosphatidylinositol, is also present in this preparation (4). The Mr 67,000 and 48,000 peptides do not appear to be absolutely necessary for autophosphorylation since phosphorylation occurs on protein kinase renatured after SDS-PAGE (3 (Fig.…”

Section: Membrane Isolation and Solubilizationmentioning

confidence: 99%

Rapid Cycling of Autophosphorylation of a Ca²⁺-Calmodulin Regulated Plasma Membrane Located Protein Kinase from Pea

Blowers¹,

Trewavas²

1989

Plant Physiol.

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Plasma membrane vesicles from pea (Pisum sativum L.) seedlings contain an autophosphorylating calcium-activated protein kinase of relative molecular weight 18,000 (phosphoprotein 18, pp18). Pulse chase analysis revealed that pp18 autophosphorylation exhibited very rapid tumover. pp18 was the only detectable plasma membrane protein to have this property. pp18 has been highly purified by affinity chromatography and the final preparation contains peptides of relative molecular weight 67,000,48,000, and 18,000. Highly purified pp18 still showed rapid cycling of autophosphorylated phosphate attached to pp18. Tumover of pp18 autophosphorylation was accelerated by ADP, but this effect did not appear to be the back reaction of the kinase since inorganic phosphate accumulation is increased by ADP. A rapid cycling of phosphorylation is an ideal control point in signal transduction.

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Phosphatidylinositol kinase activity of a plasma membrane‐associated calcium‐activated protein kinase from pea

Cited by 12 publications

References 13 publications

Characterization of a Low Molecular Mass Autophosphorylating Protein in Cultured Sugarcane Cells and Its Identification as a Nucleoside Diphosphate Kinase

Characterization of a Low Molecular Mass Autophosphorylating Protein in Cultured Sugarcane Cells and Its Identification as a Nucleoside Diphosphate Kinase

Factores nutricionales y alteración en el contenido de antocianinas relacionados con el ennegrecimiento de los pétalos en rosa (Rosa sp.)

Rapid Cycling of Autophosphorylation of a Ca²⁺-Calmodulin Regulated Plasma Membrane Located Protein Kinase from Pea

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Phosphatidylinositol kinase activity of a plasma membrane‐associated calcium‐activated protein kinase from pea

Cited by 12 publications

References 13 publications

Characterization of a Low Molecular Mass Autophosphorylating Protein in Cultured Sugarcane Cells and Its Identification as a Nucleoside Diphosphate Kinase

Characterization of a Low Molecular Mass Autophosphorylating Protein in Cultured Sugarcane Cells and Its Identification as a Nucleoside Diphosphate Kinase

Factores nutricionales y alteración en el contenido de antocianinas relacionados con el ennegrecimiento de los pétalos en rosa (Rosa sp.)

Rapid Cycling of Autophosphorylation of a Ca2+-Calmodulin Regulated Plasma Membrane Located Protein Kinase from Pea

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Rapid Cycling of Autophosphorylation of a Ca²⁺-Calmodulin Regulated Plasma Membrane Located Protein Kinase from Pea