Phosphatidylserine externalization by apoptotic cells is dispensable for specific recognition leading to innate apoptotic immune responses

Gomes, M. Teixeira; Palasiewicz, Karol; Gadiyar, Varsha; Lahey, Kevin C.; Calianese, David; Birge, Raymond B.; Ucker, David S.

doi:10.1016/j.jbc.2022.102034

Cited by 10 publications

(4 citation statements)

References 79 publications

(174 reference statements)

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“…This fluorescent dye‐based assay utilizes two markers: Annexin V and 7‐ aminoactinomycin D (7‐AAD). Annexin V is a calcium‐dependent phospholipid‐binding protein commonly used to detect early apoptotic cells by its ability to bind to phosphatidylserine, a marker of apoptosis, [45] while 7‐ aminoactinomycin D (7‐AAD) is a fluorescent DNA intercalator and an indicator of cell membrane structural integrity used to stain the dead cells [46,47] . Iriti et al [48] .…”

Section: Resultsmentioning

confidence: 99%

“…Annexin V is a calcium-dependent phospholipid-binding protein commonly used to detect early apoptotic cells by its ability to bind to phosphatidylserine, a marker of apoptosis, [45] while 7-aminoactinomycin D (7-AAD) is a fluorescent DNA intercalator and an indicator of cell membrane structural integrity used to stain the dead cells. [46,47] Iriti et al [48] reported a similar methodology using MUSE Annexin V and Dead cell kit to analyze the apoptotic effect of Rutin, a flavonoid, on MDA-MB-231 cells.…”

Section: Va-npdas-treatment Promoted Apoptosis In Human Breast Cancer...mentioning

confidence: 99%

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Antiproliferative and Apoptotic Effects of pH‐Responsive Veratric Acid‐Loaded Polydopamine Nanoparticles in Human Triple Negative Breast Cancer Cells

Harini

Ganesh

Anushikaa

et al. 2023

Chemistry & Biodiversity

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Veratric acid (VA) is plant‐derived phenolic acid known for its therapeutic potential, but its anticancer effect on highly invasive triple‐negative breast cancer (TNBC) is yet to be evaluated. Polydopamine nanoparticles (nPDAs) were chosen as the drug carrier to overcome VA's hydrophobic nature and ensure a sustained release of VA. We prepared pH‐sensitive nano‐formulations of VA‐loaded nPDAs and subjected them to physicochemical characterization and in vitro drug release studies, followed by cell viability and apoptotic assays on TNBC cells (MDA‐MB‐231 cells). The SEM and zeta analysis revealed spherical nPDAs were uniform size distribution and good colloidal stability. In vitro drug release from VA‐nPDAs was sustained, prolonged and pH‐sensitive, which could benefit tumor cell targeting. MTT and cell viability assays showed that VA‐nPDAs (IC50=17.6 μM) are more antiproliferative towards MDA‐MB‐231 cells than free VA (IC50=437.89 μM). The induction of early and late apoptosis by VA‐nPDAs in the cancer cells was identified using annexin V and dead cell assay. Thus, the pH response and sustained release of VA from nPDAs showed the potential to enter the cell, inhibit cell proliferation, and induce apoptosis in human breast cancer cells, indicating the anticancer potential of VA.

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Section: Resultsmentioning

confidence: 99%

Section: Va-npdas-treatment Promoted Apoptosis In Human Breast Cancer...mentioning

confidence: 99%

Antiproliferative and Apoptotic Effects of pH‐Responsive Veratric Acid‐Loaded Polydopamine Nanoparticles in Human Triple Negative Breast Cancer Cells

Harini

Ganesh

Anushikaa

et al. 2023

Chemistry & Biodiversity

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“…A complicated physiological process known as apoptosis is responsible for the elimination and removal of cells that are not wanted in the body ( 56 – 58 ). As a result, this study is being carried out in order to classify and evaluate the population of cells after said cells have been treated with chemotherapeutic agents.…”

Section: Resultsmentioning

confidence: 99%

Development of D-α-Tocopherol polyethylene glycol 1000 succinate fabricated nanostructural lipid carrier of sorafenib tosylate for metastatic colorectal targeting application: Stability, physical characterization, cytotoxicity, and apoptotic studies against SW48 cells PTEN

2022

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The study aimed to create D-α-Tocopherol polyethylene glycol 1000 succinate (TPGS) nanostructured lipid carriers (NLC) of sorafenib tosylate (ST) as lymphatic delivery systems (LDDS) to fight Metastatic colorectal cancer. Initially, ST-SLN, ST-NLC, and ST-LNE were formulated considering oleic acid (OA), glycerol monolinoleate (GMO), glycerol monolinoleate (GML) as solid lipid and further characterised, and tested for stability. The most stable ST-NLC was fabricated with TPGS to produce ST-TPGS-NLC and evaluated by performing in vitro drug profiling, in vitro cytotoxicity, and apoptotic studies against human female colorectal adenocarcinoma cell lines (SW48 Cells PTEN). Stability studies on three lipidic nanoparticles (ST-SLN, ST-NLC, ST-LEN) showed particle size, polydispersity index, and zeta potential ranging from 165 nm to 298 nm, 0.125 to 0.288, and -31 mV to -16 mV. At 1600 minutes, more than 80% of ST-NLC1 was released, confirming the sustained release pattern of the formulation. ST-NLC and ST-TPGS-NLC have entrapment efficiencies above 50%. Pure ST’s IC50 at 72 hr was 3.45 µg/mL, while 1.56 µg/mL was for ST-TPGS-NLC. The ST-TPGS-NLC reduced the number of livings SW48 Cells PTEN from 91% to 5%, compared to 75% to 8% of pure ST. The ST-TPGS-NLC is a promising LDDS for delivering ST for metastatic colorectal cancer.

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“…In addition to changes in the cell membrane structure consisting in phosphatidylserine (PS) externalization, the induction of caspases is another biochemical marker of apoptosis [ 80 ]. The activity of these enzymes is associated with both cell death and the inflammation response, and the caspase cascade can be initiated by various factors [ 81 ].…”

Section: Discussionmentioning

confidence: 99%

Effect of Antibiotic Amphotericin B Combinations with Selected 1,3,4-Thiadiazole Derivatives on RPTECs in an In Vitro Model

Dróżdż

Sławińska-Brych

Kubera

et al. 2022

IJMS

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4-(5-methyl-1,3,4-thiadiazole-2-yl) benzene-1,3-diol (C1) and 4-[5-(naphthalen-1-ylmethyl)-1,3,4-thiadiazol-2-yl] benzene1,3-diol (NTBD) are representative derivatives of the thiadiazole group, with a high antimycotic potential and minimal toxicity against normal human fibroblast cells. The present study has proved its ability to synergize with the antifungal activity of AmB. The aim of this work was to evaluate the cytotoxic effects of C1 or NTBD, alone or in combination with AmB, on human renal proximal tubule epithelial cells (RPTECs) in vitro. Cell viability was assessed with the MTT assay. Flow cytometry and spectrofluorimetric techniques were used to assess the type of cell death and production of reactive oxygen species (ROS), respectively. The ELISA assay was performed to measure the caspase-2, -3, and -9 activity. ATR-FTIR spectroscopy was used to evaluate biomolecular changes in RPTECs induced by the tested formulas. The combinations of C1/NTBD and AmB did not exert a strong inhibitory effect on the viability/growth of kidney cells, as evidenced by the negligible changes in the apoptotic/necrotic rate and caspase activity, compared to the control cells. Both NTBD and C1 displayed stronger anti-oxidant activity when combined with AmB. The relatively low nephrotoxicity of the thiadiazole derivative combinations and the protective activity against AmB-induced oxidative stress may indicate their potential use in the therapy of fungal infections.

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Phosphatidylserine externalization by apoptotic cells is dispensable for specific recognition leading to innate apoptotic immune responses

Cited by 10 publications

References 79 publications

Antiproliferative and Apoptotic Effects of pH‐Responsive Veratric Acid‐Loaded Polydopamine Nanoparticles in Human Triple Negative Breast Cancer Cells

Antiproliferative and Apoptotic Effects of pH‐Responsive Veratric Acid‐Loaded Polydopamine Nanoparticles in Human Triple Negative Breast Cancer Cells

Development of D-α-Tocopherol polyethylene glycol 1000 succinate fabricated nanostructural lipid carrier of sorafenib tosylate for metastatic colorectal targeting application: Stability, physical characterization, cytotoxicity, and apoptotic studies against SW48 cells PTEN

Effect of Antibiotic Amphotericin B Combinations with Selected 1,3,4-Thiadiazole Derivatives on RPTECs in an In Vitro Model

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