Phosphoenolpyruvate Synthetase from the Hyperthermophilic Archaeon
            <i>Pyrococcus furiosus</i>

Hutchins, Andrea M.; Holden, James F.; Adams, Michael W. W.

doi:10.1128/jb.183.2.709-715.2001

Cited by 48 publications

(52 citation statements)

References 55 publications

(73 reference statements)

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“…The enzymatic characterization of PEPS from Pyr. furiosus confirmed that the enzyme reaction is reversible but showed a clear preference (higher catalytic efficiency) for the gluconeogenic direction of PEP formation (324). A genetic analysis of the function of both the PEPS and PK enzymes in the EMP pathway of Tco.…”

Section: Thermococcales (Pyrococcus Furiosus and Thermococcus Kodakarmentioning

confidence: 87%

“…kodakarensis on pyruvate and the kinetic properties of the Pyr. furiosus enzyme showing higher catalytic efficiencies for pyruvate and ATP than for PEP and AMP (323,324).…”

Section: Phosphoenolpyruvate Synthetasementioning

confidence: 99%

“…Also, the Pyr. furiosus enzyme has been reported to be an octamer with a tendency to form higher inactive aggregates (324 (315,324). Like the pyruvate:phosphate dikinases (PPDKs) (see below), PEPSs belong to the PEP-utilizing enzyme family containing the conserved signature pattern, as revealed by sequence comparison (PROSITE database accession numbers PS00370 and PS00742) (325,326).…”

Section: Phosphoenolpyruvate Synthetasementioning

confidence: 99%

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Carbohydrate Metabolism in Archaea: Current Insights into Unusual Enzymes and Pathways and Their Regulation

Bräsen

Esser

Rauch

et al. 2014

Microbiol Mol Biol Rev

280

294

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SUMMARY The metabolism of Archaea , the third domain of life, resembles in its complexity those of Bacteria and lower Eukarya . However, this metabolic complexity in Archaea is accompanied by the absence of many “classical” pathways, particularly in central carbohydrate metabolism. Instead, Archaea are characterized by the presence of unique, modified variants of classical pathways such as the Embden-Meyerhof-Parnas (EMP) pathway and the Entner-Doudoroff (ED) pathway. The pentose phosphate pathway is only partly present (if at all), and pentose degradation also significantly differs from that known for bacterial model organisms. These modifications are accompanied by the invention of “new,” unusual enzymes which cause fundamental consequences for the underlying regulatory principles, and classical allosteric regulation sites well established in Bacteria and Eukarya are lost. The aim of this review is to present the current understanding of central carbohydrate metabolic pathways and their regulation in Archaea . In order to give an overview of their complexity, pathway modifications are discussed with respect to unusual archaeal biocatalysts, their structural and mechanistic characteristics, and their regulatory properties in comparison to their classic counterparts from Bacteria and Eukarya . Furthermore, an overview focusing on hexose metabolic, i.e., glycolytic as well as gluconeogenic, pathways identified in archaeal model organisms is given. Their energy gain is discussed, and new insights into different levels of regulation that have been observed so far, including the transcript and protein levels (e.g., gene regulation, known transcription regulators, and posttranslational modification via reversible protein phosphorylation), are presented.

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Section: Thermococcales (Pyrococcus Furiosus and Thermococcus Kodakarmentioning

confidence: 87%

“…kodakarensis on pyruvate and the kinetic properties of the Pyr. furiosus enzyme showing higher catalytic efficiencies for pyruvate and ATP than for PEP and AMP (323,324).…”

Section: Phosphoenolpyruvate Synthetasementioning

confidence: 99%

Section: Phosphoenolpyruvate Synthetasementioning

confidence: 99%

See 1 more Smart Citation

Carbohydrate Metabolism in Archaea: Current Insights into Unusual Enzymes and Pathways and Their Regulation

Bräsen

Esser

Rauch

et al. 2014

Microbiol Mol Biol Rev

280

294

View full text Add to dashboard Cite

show abstract

“…Similar proteins were also found among the fosmids from the deep Mediterranean libraries (Deschamps et al, 2014) but they were absent in the MG2-GG3 or any other MGII genomic fragment. Therefore, further investigations must be performed in this direction to clarify if the EMP functions in the gluconeogenic direction rather than the glycolytic pathway, as has been proposed for other Archaea (Hutchins et al, 2001;Hallam et al, 2006). Along these lines, typical gluconeogenesis enzymes such as pyruvate carboxylase (subunits A and B) and a gene coding for a phosphoenolpyruvate carboxykinase, both typical gluconeogenic enzymes, were found.…”

Section: Inferred Metabolic and Ecological Featuresmentioning

confidence: 99%

A new class of marine Euryarchaeota group II from the mediterranean deep chlorophyll maximum

et al. 2014

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We have analyzed metagenomic fosmid clones from the deep chlorophyll maximum (DCM), which, by genomic parameters, correspond to the 16S ribosomal RNA (rRNA)-defined marine Euryarchaeota group IIB (MGIIB). The fosmid collections associated with this group add up to 4 Mb and correspond to at least two species within this group. From the proposed essential genes contained in the collections, we infer that large sections of the conserved regions of the genomes of these microbes have been recovered. The genomes indicate a photoheterotrophic lifestyle, similar to that of the available genome of MGIIA (assembled from an estuarine metagenome in Puget Sound, Washington Pacific coast), with a proton-pumping rhodopsin of the same kind. Several genomic features support an aerobic metabolism with diversified substrate degradation capabilities that include xenobiotics and agar. On the other hand, these MGIIB representatives are non-motile and possess similar genome size to the MGIIA-assembled genome, but with a lower GC content. The large phylogenomic gap with other known archaea indicates that this is a new class of marine Euryarchaeota for which we suggest the name Thalassoarchaea. The analysis of recruitment from available metagenomes indicates that the representatives of group IIB described here are largely found at the DCM (ca. 50 m deep), in which they are abundant (up to 0.5% of the reads), and at the surface mostly during the winter mixing, which explains formerly described 16S rRNA distribution patterns. Their uneven representation in environmental samples that are close in space and time might indicate sporadic blooms.

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“…1) Most of the hyperthermophiles are known to be anaerobic organisms and to obtain energy by fermentation or a nonoxygenic respiratory system. Aeropyrum pernix K1 isolated from a coastal solfataric vent is a unique hyperthermophilic archaeon which grows heterotrophically at the optimum temperature of 90-959 C under absolutely aerobic conditions.…”

mentioning

confidence: 99%

Temperature Dependence of Kinetic Parameters for Hyperthermophilic Glutamate Dehydrogenase fromAeropyrum pernixK1

Bhuiya

Sakuraba

Ohshima

2002

Bioscience, Biotechnology, and Biochemistry

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Phosphoenolpyruvate Synthetase from the Hyperthermophilic Archaeon Pyrococcus furiosus

Cited by 48 publications

References 55 publications

Carbohydrate Metabolism in Archaea: Current Insights into Unusual Enzymes and Pathways and Their Regulation

Carbohydrate Metabolism in Archaea: Current Insights into Unusual Enzymes and Pathways and Their Regulation

A new class of marine Euryarchaeota group II from the mediterranean deep chlorophyll maximum

Temperature Dependence of Kinetic Parameters for Hyperthermophilic Glutamate Dehydrogenase fromAeropyrum pernixK1

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