Phosphofructokinase from muscle of the marine giant barnacle Austromegabalanus psittacus: Kinetic characterization and effect of in vitro phosphorylation

Simpfendörfer, Robert W.; Oelckers, Karin B.; López, Daniel

doi:10.1016/j.cbpc.2005.11.019

Cited by 4 publications

(3 citation statements)

References 34 publications

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“…On the other hand, AMP cannot did not modify neither glycolytic activity in COCs nor oxidative and mitochondrial activities in oocytes. The concentration of AMP used in this study was similar or higher than that reported previously to be effective for stimulating phosphofructokinase1 [31][32][33].…”

Section: Discussionsupporting

confidence: 75%

Implications of glycolytic and pentose phosphate pathways on the oxidative status and active mitochondria of the porcine oocyte during IVM

et al. 2016

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Glycolysis and the pentose phosphate pathway (PPP) were modulated in porcine cumulus-oocyte complexes during IVM by the addition of inhibitors and stimulators of key enzymes of the pathways to analyze their influence on the oxidative status, active mitochondria, and maturation of the oocyte. The influence of pharmacologic and physiological inhibitors of glycolysis (Sodium fluoride and ATP) and PPP (6-Aminonicotinamide and nicotinamide adenine dinucleotide phosphate) was validated by assessing glucose and lactate turnover and brilliant cresyl blue staining in oocytes. Inhibitors of glycolysis and PPP activity significantly perturbed nuclear maturation, oxidative metabolism (Redox Sensor Red CC-1), and active mitochondria (Mitotracker Green FM) within oocytes (P < 0.05). In comparison, physiological stimulators of glycolysis (adenosine monophosphate) and PPP (nicotinamide adenine dinucleotide phosphate) did not affect any of evaluated parameter. In the absence of modulators, fluctuations in the oocyte oxidative activity and active mitochondria were observed during porcine IVM. The inhibition of glycolysis and PPP modified the pattern of oxidation and mitochondrial fluctuation, resulting in impaired meiotic progression. We demonstrated the relationship between carbohydrate metabolism in COC and oocyte redox status necessary for porcine oocyte IVM.

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Section: Discussionsupporting

confidence: 75%

Implications of glycolytic and pentose phosphate pathways on the oxidative status and active mitochondria of the porcine oocyte during IVM

et al. 2016

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“…It is important to remark that the concentrations of AMP evaluated in this study were either the same as or higher than the ones reported previously to be effective for stimulating phosphofructokinase 1 (Simpfendorfer et al. ; Kamp et al. ).…”

Section: Discussionsupporting

confidence: 66%

“…The AMP has been identified as a positive allosteric effector of the main key enzyme of glycolysis (Harris ; Simpfendorfer et al. ; Kamp et al. ).…”

Section: Discussionmentioning

confidence: 99%

Modulation of Glycolysis and the Pentose Phosphate Pathway Influences Porcine Oocyte In Vitro Maturation

Álvarez

Ferretti

Gutnisky

et al. 2012

Reprod Domestic Animals

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Contents Glycolytic and pentose phosphate pathway (PPP) activities were modulated in porcine cumulus–oocyte complexes (COCs) during in vitro maturation (IVM) by the addition of inhibitors or stimulators of key enzymes of the pathways to elucidate their relative participation in oocyte maturation. The activities of glycolysis and PPP were evaluated by lactate production per COC and by the brilliant cresyl blue test, respectively. Glucose uptake per COC and the oocyte maturation rate were also evaluated. Lactate production, glucose uptake and the percentage of oocytes reaching metaphase II decreased in a dose‐dependent manner in the presence of the pharmacological (NaF) or the physiological (ATP) inhibitors of glycolysis (p < 0.05). The addition of the physiological stimulator of glycolysis (AMP) caused no effect on lactate production, glucose uptake or the meiotic maturation rate. The pharmacological (6‐AN) and the physiological (NADPH) inhibitors of PPP induced a dose‐dependent decrease in the percentage of oocytes with high PPP activity and in the nuclear maturation rate (p < 0.05). The physiological stimulator of PPP (NADP) caused no effect on the percentage of oocytes with high PPP activity. The glycolytic and PPP activities of porcine COCs and maturational competence of oocytes seem to be closely related events. This study shows for the first time the regulatory effect of ATP and NADPH as physiological inhibitors of glycolysis and PPP in porcine COCs, respectively. Besides, these pathways seem to reach their maximum activities in porcine COCs during IVM because no further increases were achieved by the presence of AMP or NADP.

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Diversification of Chilean aquaculture: the case of the giant barnacle Austromegabalanus psittacus (Molina, 1782)

López

Arriagada

et al. 2012

lajar

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ABSTRACT.Evidence is presented supporting the technical and economic possibilities of giant barnacle Austromegabalanus psittacus (Molina, 1782) culture, one of the main alternatives for diversifying aquaculture in Chile. Spat collection from the wild varied between different sites in the north and south of the country and according to type of artificial collector. Growth also varied between sites (greater in the north), technological systems (greater in tubular systems) and depths (greater at 4 m). Average commercial size in the national market was reached over a period between 18 and 24 months. A long-line can produce between 7 to 10 gross ton during this period, therefore average annual fisheries production can be reached with only 10 to 30 longlines, in an area of 1 to 3 ha. There is demand for this resource in the external market, particularly in the Japanese market, either as product similar to "fujit subo" (Balanus rostratus), or as a new resource; the relationship between production costs and price determines that giant barnacle culture has commercial potential. Economic indicators for cultured giant barnacle were as follows: net present value (NPV): US$ 490,000; internal rate of return (IRR): 32%; discounted payback period (DPBP): 4 years. Results obtained suggest the natural bank repopulation option, and the development of mass cultures. Giant barnacle culture is based on biological characteristics that differentiate it from other crustaceans species, as well as simple and economic production technologies and favourable economic projections on external markets. Keywords: Austromegabalanus psittacus, barnacle culture, spat collection, commercial size, aquaculture diversification, Chile. Diversificación de la acuicultura chilena: el caso del cirripedio giganteAustromegabalanus psittacus (Molina, 1782) RESUMEN. Se presentan evidencias de las posibilidades técnicas y económicas del cultivo del cirripedio gigante o "picoroco", Austromegabalanus psittacus (Molina, 1782). Esta especie es una de las principales alternativas para la diversificación de la acuicultura en Chile. La captación de semilla desde el ambiente varió entre distintos sitios del norte y sur del país y según el tipo de colector artificial. El crecimiento también varió entre localidades (mayor en el norte del país), el tipo de sistema (mayor en sistemas tubulares) y profundidad (mayor a 4 m). La talla comercial promedio del mercado nacional se alcanzó entre 18 y 24 meses. Un longline puede producir entre 7 y 10 ton brutas en ese periodo, por lo que la producción promedio anual por pesquería se puede alcanzar con sólo 10 a 30 long-lines, en un área de 1 a 3 ha. Existe demanda de este recurso en el mercado externo particularmente en el mercado japonés sea como símil del "fujit subo" (Balanus rostratus) o como recurso nuevo; las relaciones de costos de producción y precios indican que tienen potencialidades en cultivos comerciales. Los indicadores económicos para el picoroco congelado proveniente de cultivo fueron los siguientes: el valor actuali...

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Phosphofructokinase from muscle of the marine giant barnacle Austromegabalanus psittacus: Kinetic characterization and effect of in vitro phosphorylation

Cited by 4 publications

References 34 publications

Implications of glycolytic and pentose phosphate pathways on the oxidative status and active mitochondria of the porcine oocyte during IVM

Implications of glycolytic and pentose phosphate pathways on the oxidative status and active mitochondria of the porcine oocyte during IVM

Modulation of Glycolysis and the Pentose Phosphate Pathway Influences Porcine Oocyte In Vitro Maturation

Diversification of Chilean aquaculture: the case of the giant barnacle Austromegabalanus psittacus (Molina, 1782)

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