Phosphofructokinase in Relation to Sugar Accumulation in Cold-Stored Potato Tubers

Bredemeijer, G. M. M.; Burg, H.C.J.; Claassen, P.A.M.; Stiekema, Willem J.

doi:10.1016/s0176-1617(11)80258-0

Cited by 13 publications

(3 citation statements)

References 22 publications

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“…3) equally feasible mechanism for dealing with torpid kinetics of certain enzymes at low temperature, that of increasing the amount of the enzyme, would not be sufficient to maintain homeostatic flux (4). However, what little is known about the few enzyme systems in plants acclimated to low temperature would contradict the hypothesis of sucrose accumulation based on a simple kinetic argument (9,11).…”

Section: Resultsmentioning

confidence: 99%

Sucrose Phosphate Synthase and Sucrose Accumulation at Low Temperature

Guy¹,

Huber²,

Huber³

1992

Plant Physiol.

355

201

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The influence of growth temperature on the free sugar and sucrose phosphate synthase content and activity of spinach (Spinacia oleracea) leaf tissue was studied. When plants were grown at 25 C for 3 weeks and then transferred to a constant 5 C, sucrose, glucose, and fructose accumulated to high levels during a 14-d period. Predawn sugar levels increased from 14-to 20-fold over the levels present at the outset of the low-temperature treatment. Sucrose was the most abundant free sugar before, during, and after exposure to 5°C. Leaf sucrose phosphate synthase activity was significantly increased by the low-temperature treatment, whereas sucrose synthase and invertases were not. Synthesis of the sucrose phosphate synthase subunit was increased during and after lowtemperature exposure and paralleled an increase in the steadystate level of the subunit. The increases in sucrose and its primary biosynthetic enzyme, sucrose phosphate synthase, are discussed in relation to adjustment of metabolism to low nonfreezing temperature and freezing stress tolerance.

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Section: Resultsmentioning

confidence: 99%

Sucrose Phosphate Synthase and Sucrose Accumulation at Low Temperature

Guy¹,

Huber²,

Huber³

1992

Plant Physiol.

355

201

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“…3). The observation that the accumulation of sucrose and reducing sugars in potatoes during cold storage correlates with a decrease in activity of the cytosolic isozyme of PFK (14) suggests that it has an important role in the interconversion of starch and sucrose and has led to interest in the possibility of eliminating this undesirable trait through genetic engineering.…”

Section: Interconversion Of Fru-6-p and Fru-1 6-p2mentioning

confidence: 99%

Molecular approaches to the manipulation of carbon allocation in plants

Blakeley¹,

Dennis²

1993

Can. J. Bot.

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1993. Molecular approaches to the manipulation of carbon allocation in plants. Can. J. Bot. 71: 765-778.In plants, sucrose is the end product of photosynthesis and is converted to a wide variety of storage compounds in tissues such as seeds and tubers. The allocation of carbon from sucrose to the various metabolic pathways leading to these products will determine the quantity of each synthesized in the respective storage organs. If the level of the enzymes involved in the allocation of carbon could be changed by genetic manipulation, it is probable that the relative yields of the various storage products can also be altered. The initial breakdown of sucrose occurs in the cytosol of the cell. Many biosynthetic pathways, however, including those involved in the synthesis of storage products such as fatty acids, starch, and amino acids, occur in the plastid. The distribution of carbon substrates for these processes will be determined, to a large extent, by the flux of carbon through the glycolytic pathways found in both the cytosolic and plastid compartments. This article will discuss the importance and consequences of compartmentation, review the extent of our understanding of glycolysis and other enzymes and pathways regulating carbon allocation, and will speculate on the potential for the genetic manipulation of these pathways. BLAKELEY, S.D., et DENNIS, D.T. 1993. Molecular approaches to the manipulation of carbon allocation in plants. Can.J. Bot. 71 : 765-778. Chez les plantes, le sucrose est le dernier produit de la photosynthkse qui est par la suite transform6 en une gamme de substances de rtserve dans les tissus des graines et des tubercules, B titre d'exemple. La distribution du carbone sous forme de sucrose dans les diffkrents sentiers mitaboliques conduisant B ces produits, dCterminera la quantitC de ceux-~i synthCtisCs dans les organes de reserves respectifs. Si on peut changer, par manipulation gCnCtique, les teneurs des enzymes impliquees dans la distribution du carbone, il est probable qu'on modifiera tgalement les rendements relatifs de ces diverses substances de rtserve. L'hydrolyse du sucrose dCbute dans le cytosol de la cellule. Cependant, plusieurs sentiers de biosynthbse, y compris ceux qui sont impliquCs dans la synthbse des substances de reserve telles que les acides gras, l'amidon, et les acides aminks, sont localisCs dans les plastes. La distribution des substrats carbones nCcessaires pour ces diffkrents processus est dCterminCe, pour I'essentiel, par le flux de carbone dans les sentiers glycolytiques qui se trouvent dans les compartiments cytosoliques aussi bien que plastidiques. Dans leur article, les auteurs discutent l'importance et les consCquences de la compartimentation, ils revoient l'ensemble de notre comprChension de la glycolyse ainsi que des autres enzymes et sentiers impliquCs dans la regulation de la distribution du carbone et ils Cvoquent ce que la manipulation gCnCtique de ces sentiers pourrait apporter de nouveau.

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“…Bintje drops down already at temperatures below +14.9 °C. Phosphofructo-⎯⎯⎯⎯ kinase from chloroplasts is only slightly less sensitive and its inactivation substantially increases below temperature +12.5 °C (Bredemeijer et al 1991). The cytosolic form requires the pH value around 6.5 and is sensitive to the low pH values, which are commonly achieved in tubers in one week of cold storage (Bredemeijer et al 1991).…”

Section: Introductionmentioning

confidence: 99%

Decreased amount of reducing sugars in transgenic potato tubers and its influence on yield characteristics

Navrátil

Fischer²,

Čmejlová

et al. 2007

Biologia plant.

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This work focuses on the comparison of field characteristics and amounts of reducing sugars in cold-stored tubers of transgenic plants derived from two potato cultivars. The bacterial gene coding for phosphofructokinase under the tuberspecific promoter was used to support the glycolysis in stored tubers. While the tubers from untransformed control plants steadily accumulated reducing sugars during cold storage, the tubers from transformed plants regardless the genotype were characterized by subsequent decrease in the sugar content. After long period of cold storage the greatest reduction in the reducing sugar content was by more than 60 % compared to control. Before the storage, however, the content of reducing sugars was in 80 % of transgenic lines higher than in control ones. The plants evaluated in field trials for their appearance showed any changes in growth characteristics in about 25 % of the transgenic lines. Despite the introduced modification of sugar metabolism the yield of transgenic plants with normal appearance did not differ significantly from the yield of control plants.

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Phosphofructokinase in Relation to Sugar Accumulation in Cold-Stored Potato Tubers

Cited by 13 publications

References 22 publications

Sucrose Phosphate Synthase and Sucrose Accumulation at Low Temperature

Sucrose Phosphate Synthase and Sucrose Accumulation at Low Temperature

Molecular approaches to the manipulation of carbon allocation in plants

Decreased amount of reducing sugars in transgenic potato tubers and its influence on yield characteristics

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