Phosphoglycerate kinase and fructose bisphosphate aldolase of Candida albicans as new antigens recognized by human salivary IgA

Calcedo, Roberto; Ramirez‐Garcia, Andoni; Abad, Ana; Rementerı́a, Aitor; Pontón, José; Hernando, Fernando

doi:10.1016/j.riam.2011.07.004

Cited by 19 publications

(9 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A number of studies have now demonstrated that antibodies can be protective against Candida, with a number of protective monoclonals or antibody fragments (Fabs) having been described for C. albicans [356][357][358][359][360][361][362][363][364][365][366][367][368][369]. These studies demonstrated that there are multiple targets for antibodies including polysaccharides, proteins, and glycolipids.…”

Section: B Cells and Antibodymentioning

confidence: 99%

CandidaImmunity

Naglik

2014

New Journal of Science

View full text Add to dashboard Cite

The human pathogenic fungus Candida albicans is the predominant cause of both superficial and invasive forms of candidiasis. C. albicans primarily infects immunocompromised individuals as a result of either immunodeficiency or intervention therapy, which highlights the importance of host immune defences in preventing fungal infections. The host defence system utilises a vast communication network of cells, proteins, and chemical signals distributed in blood and tissues, which constitute innate and adaptive immunity. Over the last decade the identity of many key molecules mediating host defence against C. albicans has been identified. This review will discuss how the host recognises this fungus, the events induced by fungal cells, and the host innate and adaptive immune defences that ultimately resolve C. albicans infections during health.

show abstract

Section: B Cells and Antibodymentioning

confidence: 99%

CandidaImmunity

Naglik

2014

New Journal of Science

View full text Add to dashboard Cite

show abstract

“…On the other hand, Pgk1p and Fba1p were major protein antigens that reacted with human salivary IgA (Calcedo et al . ). Proteins that are seroreactive to sera from patients are potentially valuable for development of serological test and immunotherapeutics.…”

Section: Discussionmentioning

confidence: 97%

“…Eno1p and Ssb1p were among the protein antigens that were found in the blood circulation of patients with systemic candidiasis (Walsh et al 1991;Giraldo et al 1999). On the other hand, Pgk1p and Fba1p were major protein antigens that reacted with human salivary IgA (Calcedo et al 2012). Proteins that are seroreactive to sera from patients are potentially valuable for development of serological test and immunotherapeutics.…”

Section: Discussionmentioning

confidence: 99%

Identification of immunogenic proteins of Candida parapsilosis by serological proteome analysis

et al. 2014

View full text Add to dashboard Cite

Aims Systemic candidiasis is the leading fungal bloodstream infection, and its incidence has been on the rise. Recently, Candida parapsilosis has emerged as an increasingly prevalent fungal pathogen, but little is known about its antigenic profile. Hence, the current work was performed to discover immunogenic proteins of C. parapsilosis using serological proteome analysis. Methods and Results Cell wall proteins extracted from C. parapsilosis were resolved by two‐dimensional electrophoresis followed by immunoblotting using antisera from experimentally infected mice. Mass spectrometry analysis of the 32 immunoreactive protein spots resulted in the identification of 12 distinct proteins. Among them, 11 proteins were known antigens of Candida albicans, whereas Idh2p was identified for the first time as an immunogenic protein of Candida species. Recombinant Idh2p was expressed in Escherichia coli, and its antigenicity was verified by immunoblot analysis. Conclusions An immunoproteomic approach was successfully applied to identify immunogenic proteins of C. parapsilosis, with Idh2p as a novel candidate antigen. The identified antigens may serve as potential biomarkers for development of diagnostic assay and/or vaccine for C. parapsilosis. Significance and Impact of the Study This work represents the first immunoproteomic analysis of C. parapsilosis, which provides new insights into host–pathogen interactions and pathogenesis of C. parapsilosis. The immunogenic proteins could be studied as biomarker candidates for C. parapsilosis.

show abstract

“…zooepidemicus through the blood-brain barrier. Finally, fructose-bisphosphate aldolase has been reported to be immunogenic in Candida albicans (Calcedo et al, 2012) and Madurella mycetomatis , in which it has been proposed as a vaccine candidate (de Klerk et al, 2012). …”

Section: Discussionmentioning

confidence: 99%

Brachyspira hyodysenteriae and B. pilosicoli Proteins Recognized by Sera of Challenged Pigs

et al. 2017

View full text Add to dashboard Cite

The spirochetes Brachyspira hyodysenteriae and B. pilosicoli are pig intestinal pathogens that are the causative agents of swine dysentery (SD) and porcine intestinal spirochaetosis (PIS), respectively. Although some inactivated bacterin and recombinant vaccines have been explored as prophylactic treatments against these species, no effective vaccine is yet available. Immunoproteomics approaches hold the potential for the identification of new, suitable candidates for subunit vaccines against SD and PIS. These strategies take into account the gene products actually expressed and present in the cells, and thus susceptible of being targets of immune recognition. In this context, we have analyzed the immunogenic pattern of two B. pilosicoli porcine isolates (the Spanish farm isolate OLA9 and the commercial P43/6/78 strain) and one B. hyodysenteriae isolate (the Spanish farm V1). The proteins from the Brachyspira lysates were fractionated by preparative isoelectric focusing, and the fractions were analyzed by Western blot with hyperimmune sera from challenged pigs. Of the 28 challenge-specific immunoreactive bands detected, 21 were identified as single proteins by MS, while the other 7 were shown to contain several major proteins. None of these proteins were detected in the control immunoreactive bands. The proteins identified included 11 from B. hyodysenteriae and 28 from the two B. pilosicoli strains. Eight proteins were common to the B. pilosicoli strains (i.e., elongation factor G, aspartyl-tRNA synthase, biotin lipoyl, TmpB outer membrane protein, flagellar protein FlaA, enolase, PEPCK, and VspD), and enolase and PEPCK were common to both species. Many of the identified proteins were flagellar proteins or predicted to be located on the cell surface and some of them had been previously described as antigenic or as bacterial virulence factors. Here we report on the identification and semiquantitative data of these immunoreactive proteins which constitute a unique antigen collection from these bacteria.

show abstract

Phosphoglycerate kinase and fructose bisphosphate aldolase of Candida albicans as new antigens recognized by human salivary IgA

Cited by 19 publications

References 19 publications

CandidaImmunity

CandidaImmunity

Identification of immunogenic proteins of Candida parapsilosis by serological proteome analysis

Brachyspira hyodysenteriae and B. pilosicoli Proteins Recognized by Sera of Challenged Pigs

Contact Info

Product

Resources

About