Phospholipase B activity and organophosphorus compound toxicity in cultured neural cells

Read, David; Langford, Lynda; Barbour, Helen; Forshaw, Philip J.; Glynn, Paul

doi:10.1016/j.taap.2006.08.002

Cited by 12 publications

(8 citation statements)

References 21 publications

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“…These include human neuroblastoma (SH-SY5Y and variants) (Ehrich et al, 1994; Hong et al, 2003; Massicotte et al, 2005; Nostrandt and Ehrich, 1992; Pope et al, 1995; Veronesi and Ehrich, 1993); mouse neuroblastoma (NB41A3) (Veronesi and Ehrich, 1993); human cervical adenocarcinoma (HeLa); rat adrenal pheochromocytoma (PC-12) and rat glioma (C6) (Li and Casida, 1997; 1998); bovine and porcine adrenal medullary chromaffin cells (Romero et al, 2006); mouse primary cortical and cerebellar granule cells and astrocytes (Read et al, 2007); mouse inner medullary collecting duct cells (Gallazzini et al, 2006); and, most recently, mouse embryonic stem cells (Pamies et al, 2010). …”

Section: Discussionmentioning

confidence: 99%

Motor neuron disease due to neuropathy target esterase mutation: Enzyme analysis of fibroblasts from human subjects yields insights into pathogenesis

et al. 2010

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Recently, we identified neuropathy target esterase (NTE) mutation as the cause of an autosomal recessive motor neuron disease (NTE-MND). Subsequently, we showed that NTE-MND mutations reduced specific activity (SA) and altered inhibitory kinetics of NTE catalytic domain constructs. Recent preliminary results showed that NTE is expressed in cultured human skin fibroblasts, and others have used mutant forms of neuronal proteins expressed in fibroblasts as biomarkers of neurogenetic diseases. Therefore, the present study was carried out to test the hypothesis that NTE in cultured skin fibroblasts from NTE-MND subjects also exhibit altered enzymological properties assessed by SA and IC50 values of mipafox (MIP) and chlorpyrifos oxon (CPO). NTE SA was reduced to 65% of control (wild type NTE from commercially obtained fibroblasts) in homozygous M1012V fibroblasts and 59-61% of control in compound heterozygous R890H/c2946_2947InsCAGC fibroblasts. MIP IC50 values were unaffected by the NTE mutations, but the CPO IC50 increased 4.5-fold in homozygous M1012V fibroblasts. Interestingly, markedly reduced NTE SAs (40-43% of control) were observed in fibroblasts from asymptomatic subjects heterozygous for NTE insertion c2946_2947InsCAGC. This insertion is predicted to produce truncated NTE missing the last 235 residues of its catalytic domain. These observations confirm that NTE-MND mutations reduce NTE SA in vitro. Moreover, to the extent observations made in cultured fibroblasts may be generalized to events in the nervous system, lack of correlation between reduced fibroblast NTE SA and the occurrence of NTE-MND in NTE insertion mutation heterozygotes indicates that reduction of NTE SA alone is insufficient to cause MND.

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Section: Discussionmentioning

confidence: 99%

Motor neuron disease due to neuropathy target esterase mutation: Enzyme analysis of fibroblasts from human subjects yields insights into pathogenesis

et al. 2010

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“…By binding to pesticides, BChE reduces the amount of active substances that can interfere with AChE to induce acute toxicity, or other esterases (e.g. neuropathy target esterase) to induce chronic neurotoxicity 26 .…”

Section: Discussionmentioning

confidence: 99%

“…In fact they highlight the different roles and functions of BChE under various conditions. On the one hand, BChE acts as a bioscavenger under pesticide-exposed conditions, backing AChE and protecting the brain against toxic effects 26 . However, it has recently been discovered that BChE has its own physiological role affecting brain homeostasis 30 .…”

Section: Discussionmentioning

confidence: 99%

K-variant BCHE and pesticide exposure: Gene-environment interactions in a case–control study of Parkinson’s disease in Egypt

Rösler

Salama

Shalash

et al. 2018

Sci Rep

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Pesticide exposure is associated with increased risk of Parkinson’s disease (PD). We investigated in Egypt whether common variants in genes involved in pesticide detoxification or transport might modify the risk of PD evoked by pesticide exposure. We recruited 416 PD patients and 445 controls. Information on environmental factors was collected by questionnaire-based structured interviews. Candidate single-nucleotide polymorphisms (SNPs) in 15 pesticide-related genes were genotyped. We analyzed the influence of environmental factors and SNPs as well as the interaction of pesticide exposure and SNPs on the risk of PD. The risk of PD was reduced by coffee consumption [OR = 0.63, 95% CI: 0.43–0.90, P = 0.013] and increased by pesticide exposure [OR = 7.09, 95% CI: 1.12–44.01, P = 0.036]. The SNP rs1126680 in the butyrylcholinesterase gene BCHE reduced the risk of PD irrespective of pesticide exposure [OR = 0.38, 95% CI: 0.20–0.70, P = 0.002]. The SNP rs1803274, defining K-variant BCHE, interacted significantly with pesticide exposure (P = 0.007) and increased the risk of PD only in pesticide-exposed individuals [OR = 2.49, 95% CI: 1.50–4.19, P = 0.0005]. The K-variant BCHE reduces serum activity of butyrylcholinesterase, a known bioscavenger for pesticides. Individuals with K-variant BCHE appear to have an increased risk for PD when exposed to pesticides.

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“…Studies using cultured neural cells, yeast, Drosophila (with Swiss cheese protein, NTE's fruit fly homolog), and heterozygous NTE knockout mice have reported that NTE acts on membrane lipids and has lysophospholipase and phospholipase B activity Read et al, 2007). It deacetylates phospholipids of intracellular membranes (Glynn, 2005(Glynn, , 2006Zaccheo et al, 2004) and hydrolyzes lysophosphatidylcholine (Casida and Quistad, 2005;Casida et al, 2008;Vose et al, 2007Vose et al, , 2008.…”

Section: Op-induced Nte Inhibition Occurs In Tissues Of Animalmentioning

confidence: 99%

Organophosphorus-Induced Delayed Neuropathy

Ehrich

Jortner

2010

Hayes' Handbook of Pesticide Toxicology

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Phospholipase B activity and organophosphorus compound toxicity in cultured neural cells

Cited by 12 publications

References 21 publications

Motor neuron disease due to neuropathy target esterase mutation: Enzyme analysis of fibroblasts from human subjects yields insights into pathogenesis

Motor neuron disease due to neuropathy target esterase mutation: Enzyme analysis of fibroblasts from human subjects yields insights into pathogenesis

K-variant BCHE and pesticide exposure: Gene-environment interactions in a case–control study of Parkinson’s disease in Egypt

Organophosphorus-Induced Delayed Neuropathy

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