2002
DOI: 10.4049/jimmunol.168.11.5682
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Phospholipases D1 and D2 Regulate Different Phases of Exocytosis in Mast Cells

Abstract: The rat mast cell line RBL-2H3 contains both phospholipase D (PLD)1 and PLD2. Previous studies with this cell line indicated that expressed PLD1 and PLD2 are both strongly activated by stimulants of secretion. We now show by use of PLDs tagged with enhanced green fluorescent protein that PLD1, which is largely associated with secretory granules, redistributes to the plasma membrane in stimulated cells by processes reminiscent of exocytosis and fusion of granules with the plasma membrane. These processes and se… Show more

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Cited by 122 publications
(131 citation statements)
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“…Our results demonstrating a requirement for PLD in exocytosis confirm similar investigations using butanol or overexpression of catalytically inactive PLDs in mast cells (19), chromaffin cells (18), and neurons (17), and yet there do appear to be differences in PLD function between the various secretory cell types. In chromaffin cells and synaptosomes, in which a role in fusion is probably best supported, PLD1 is not present on secretory vesicles but rather the plasma membrane.…”
Section: Figsupporting
confidence: 79%
See 1 more Smart Citation
“…Our results demonstrating a requirement for PLD in exocytosis confirm similar investigations using butanol or overexpression of catalytically inactive PLDs in mast cells (19), chromaffin cells (18), and neurons (17), and yet there do appear to be differences in PLD function between the various secretory cell types. In chromaffin cells and synaptosomes, in which a role in fusion is probably best supported, PLD1 is not present on secretory vesicles but rather the plasma membrane.…”
Section: Figsupporting
confidence: 79%
“…In chromaffin cells and synaptosomes, in which a role in fusion is probably best supported, PLD1 is not present on secretory vesicles but rather the plasma membrane. The enzyme, at least when overexpressed, is located on secretory vesicles in mast cells (19), but its role is suggested to relate to granule translocation rather than fusion, which, in an added complexity, appears to be mediated by PLD2 on the plasma membrane. Our results place PLD1 on ␤-cell granules but implicate its function in fusion.…”
Section: Figmentioning
confidence: 99%
“…In cell lines, endogenous PLD2 has been demonstrated in the plasma membrane, Golgi apparatus, and nucleus (50,63,67). Overexpressed PLD2 has been demonstrated in the plasma membrane as well as intracellular vesicles and the nucleus (18,64,67). Like PLD1, PLD2 has been demonstrated to undergo agonist-induced translocation between membrane compartments (25,67,71,72).…”
Section: Discussionmentioning
confidence: 99%
“…Both PLD1 and PLD2 have been linked physically and functionally to the actin cytoskeleton (16,(75)(76)(77)(78)(79)(80), and each may contribute to actin's role in phagocytosis, but at different cellular sites. Such complementary, but distinctive, functions for PLD1 and PLD2 have recently been characterized in colonic epithelia (28) and mast cells (64).…”
Section: Discussionmentioning
confidence: 99%
“…There exist two mammalian PLD isoforms (PLD1 and PLD2) that occur as splice variants (Frohman et al, 1999). PLD1 appears to be localized in the Golgi or perinuclear vesicular structure (Liscovitch et al, 1999;Freyberg et al, 2001;Choi et al, 2002;Jang et al, 2004), whereas PLD2 appears to be associated with the plasma membrane (Choi et al, 2002;Exton, 2002;Sarri et al, 2003). PLD1 is known to be activated by protein kinase C (PKC), ADP-ribo-sylation factor, RalA, and RhoA in the presence of phosphatidylinositol 4, 5-bisphosphate.…”
Section: Introductionmentioning
confidence: 99%