Phospholipid-Based Signaling in Plants

Meijer, H.J.G.; Munnik, Teun

doi:10.1146/annurev.arplant.54.031902.134748

Cited by 548 publications

(535 citation statements)

References 251 publications

Supporting

Mentioning

522

Contrasting

Unclassified

Order By: Relevance

“…Concerning rafts, one can suggest that, according to animal cell data, domains may be organized in the Golgi and subsequently exported to the plasma membrane [59]. PIP 2 plays a role in the transport to the plasma membrane [60]. A PI transfer protein (PI-TP) was identified in Arabidopsis and was shown to be a SEC14 homolog allowing the complementation of a yeast sec14 mutant.…”

Section: Transfer To the Plasma Membranementioning

confidence: 99%

Glycerolipid transfer for the building of membranes in plant cells

Jouhet

Maréchal

Block

2007

Progress in Lipid Research

134

View full text Add to dashboard Cite

Section: Transfer To the Plasma Membranementioning

confidence: 99%

Glycerolipid transfer for the building of membranes in plant cells

Jouhet

Maréchal

Block

2007

Progress in Lipid Research

134

View full text Add to dashboard Cite

“…PI monophosphate 5-kinase (PIP5K) is a key enzyme in the PI signaling pathway that catalyzes the synthesis of PI-4,5-bisphosphate [PtdIns(4,5)P 2 ], which is the precursor of two important secondary messengers: inositol 1,4,5-trisphosphate [Ins(1,4,5)P 3 ] and diacylglycerol [4,5].…”

Section: Introductionmentioning

confidence: 99%

Arabidopsis phosphatidylinositol monophosphate 5-kinase 2 is involved in root gravitropism through regulation of polar auxin transport by affecting the cycling of PIN proteins

et al. 2011

View full text Add to dashboard Cite

Phosphatidylinositol monophosphate 5-kinase (PIP5K) catalyzes the synthesis of PI-4,5-bisphosphate (PtdIns(4,5) P 2 ) by phosphorylation of PI-4-phosphate at the 5 position of the inositol ring, and is involved in regulating multiple developmental processes and stress responses. We here report on the functional characterization of Arabidopsis PIP5K2, which is expressed during lateral root initiation and elongation, and whose expression is enhanced by exogenous auxin. The knockout mutant pip5k2 shows reduced lateral root formation, which could be recovered with exogenous auxin, and interestingly, delayed root gravity response that could not be recovered with exogenous auxin. Crossing with the DR5-GUS marker line and measurement of free IAA content confirmed the reduced auxin accumulation in pip5k2. In addition, analysis using the membrane-selective dye FM4-64 revealed the decelerated vesicle trafficking caused by PtdIns(4,5)P 2 reduction, which hence results in suppressed cycling of PIN proteins (PIN2 and 3), and delayed redistribution of PIN2 and auxin under gravistimulation in pip5k2 roots. On the contrary, PtdIns(4,5) P 2 significantly enhanced the vesicle trafficking and cycling of PIN proteins. These results demonstrate that PIP5K2 is involved in regulating lateral root formation and root gravity response, and reveal a critical role of PIP5K2/PtdIns(4,5)P 2 in root development through regulation of PIN proteins, providing direct evidence of crosstalk between the phosphatidylinositol signaling pathway and auxin response, and new insights into the control of polar auxin transport.

show abstract

“…PLD activity has been implicated in mediating the ABA inhibition of ␣-amylase secretion (13), ABA-regulated stomatal movement (14), and ABA-induced gene expression (15,16). Arabidopsis has 12 PLDs, and multiple types of PLDs display distinctly different catalytic and regulatory properties (17).…”

mentioning

confidence: 99%

Phospholipase Dα1-derived phosphatidic acid interacts with ABI1 phosphatase 2C and regulates abscisic acid signaling

Zhang

Qin

Zhao

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

468

459

View full text Add to dashboard Cite

A bscisic acid (ABA) plays an important role in plant growth, development, and responses to environmental stresses, such as drought, salinity, and low temperature (1). Reversible protein phosphorylation is involved in the early events of ABA signal transduction (2, 3). Specific protein kinases are activated in response to ABA and have been proposed to play a positive role in ABA signaling (2, 4). On the other hand, protein phosphatases 2C (PP2C), such as ABI1, ABI2, and AtPP2CA, are negative regulators in ABA responses (5). The loss of ABI1 or ABI2 PP2C activity in the intragenic revertants of abi1-1 or abi2-1 leads to an enhanced response to ABA (6, 7), whereas overexpression of ABI1 or AtPP2CA blocks the expression of ABAinducible genes in Arabidopsis protoplasts (3). Antisense (AS) inhibition of AtPP2CA results in the enhancement of cold-and ABA-induced gene expression (8). Recently, ABI1 was shown to interact with ATHB6, a transcriptional regulator (9), and with PKS18, a SOS2-like protein kinase (10), whereas ABI2 and AtPP2CA were found to regulate SOS2 kinase and K ϩ channels, respectively (10-12). However, how the activity and function of PP2Cs are regulated in plant cells is still unclear.Recent studies indicate that phospholipase D (PLD) is involved in ABA responses. PLD activity has been implicated in mediating the ABA inhibition of ␣-amylase secretion (13), ABA-regulated stomatal movement (14), and ABA-induced gene expression (15,16). Arabidopsis has 12 PLDs, and multiple types of PLDs display distinctly different catalytic and regulatory properties (17). Molecular and genetic data have indicated that a specific PLD participates in signaling the ABA response. AS inhibition of PLD␣1 diminished stomatal closure induced by ABA or drought and increased water loss in Arabidopsis, whereas overexpression of PLD␣1 resulted in an increased sensitivity to ABA (18). In addition, specific PLDs have been shown to regulate many other plant functions, including cell patterning, programmed cell death, and stress tolerance (19-21). However, the direct target of PLD action is unknown. In this study, we present evidence for a direct link between PLD␣1 and PP2C in the ABA signaling response. Materials and Methods Plant Materials and Growth.A T-DNA-insertional mutant of PLD␣1 (PLD␣1-KO) was identified from SALK 053785 seeds obtained from the Arabidopsis Biological Resource Center (Columbus, OH). The site of T-DNA insertion was confirmed by DNA sequencing. The generation of PLD␣1-AS plants was described in ref. 22. Seeds of PLD␣1-KO, PLD␣1-AS, and wild type of Arabidopsis thaliana (ecotype Columbia) were sown in soil and kept at 4°C for 2 days. Plants were grown in a growth chamber with cool white fluorescent light of 100 mol m Ϫ2 ⅐s Ϫ1 under 14-h light͞10-h dark and 23°C͞18°C cycles.Water Loss and Stomatal Aperture Measurements. Detached leaves from 6-week-old plants were exposed to cool white light (125 mol m Ϫ2 ⅐s Ϫ1 ) at 23°C. Leaves were weighed at various time intervals, and the loss of fresh weight (%) was used to in...

show abstract

Phospholipid-Based Signaling in Plants

Cited by 548 publications

References 251 publications

Glycerolipid transfer for the building of membranes in plant cells

Glycerolipid transfer for the building of membranes in plant cells

Arabidopsis phosphatidylinositol monophosphate 5-kinase 2 is involved in root gravitropism through regulation of polar auxin transport by affecting the cycling of PIN proteins

Phospholipase Dα1-derived phosphatidic acid interacts with ABI1 phosphatase 2C and regulates abscisic acid signaling

Contact Info

Product

Resources

About