Phospholipid composition of some amine storage granules

Filgueiras, O. M. de Oliveira; Bosch, H. van den; Johnson, Robert G.; Carty, Sally E.; Scarpa, Antonio

doi:10.1016/0014-5793(81)80190-1

Cited by 19 publications

(4 citation statements)

References 34 publications

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“…Notably, our data are to some extent consistent with the lipid levels found in chromaffin granules (Blaschko, et al, 1967; Winkler et al, 1967; Da Prada et al, 1972; Balzer and Khan, 1975; De Oliveira Filgueiras et al, 1981), where PC was 25-28% (bovine)/37% (rat), PE was 27-36% (bovine)/30% (rat), PS was 7.9-8.9% (bovine)/11% (rat), PI was 1.5-2.9% (bovine), SM was 11-20% (bovine)/9% (rat). Nevertheless, the overall composition is similar to that of the plasma membrane, which seems plausible, considering that the effect of exocytosis on PM lipid homeostasis should be limited (Sezgin et al, 2015).…”

Section: Discussionsupporting

confidence: 90%

Purification of age-distinct insulin secretory granules through antigen restriction

Neukam

Ganss

Vasiljević

et al. 2020

Preprint

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Pancreatic islet beta cells employ secretory granules for the storage and glucose-stimulated release of the hormone insulin. The competence of an insulin granule for exocytosis depends on spatial and temporal variables such as its proximity to the plasma membrane as well as its age, with newly-generated granules being preferentially released. The molecular underpinnings for the control of these variables remain largely unknown and their uncovering is of high relevance for the study of diabetes, which results from deficient insulin secretion.However, we still lack a comprehensive view about the molecular composition of the insulin granules and how this may change over their lifetime. Here we report a strategy for the background-free purification of insulin secretory granules of distinct age from insulinoma INS-1 cells. We show that utilization of an immuno-based affinity approach for pulse-chase labeled insulin secretory granules, produces a highly enriched granular fraction. Our approach precludes typical contaminants from the solid phase and may be designed to purify secretory granules of a distinct age.

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Section: Discussionsupporting

confidence: 90%

Purification of age-distinct insulin secretory granules through antigen restriction

Neukam

Ganss

Vasiljević

et al. 2020

Preprint

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“…In theory, capacitance changes could exceed fluorescence changes. For example, the behavior of FM dye molecules may be different in the surface and granule membranes, which are known to differ in biochemical composition (de Oliveira Filgueiras et al, 1981;Dreyfus et al, 1977). This is an important consideration because the fluorescence of FM1-43 is strongly influenced by its environment (Betz et al, 1996).…”

Section: Fm1-43 Fluorescence and Capacitancementioning

confidence: 99%

Exocytosis in Bovine Chromaffin Cells: Studies with Patch-Clamp Capacitance and FM1-43 Fluorescence

Kilic

2002

Biophysical Journal

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In response to physiological stimuli, neuroendocrine cells secrete neurotransmitters through a Ca(2+)-dependent fusion of secretory granules with the plasma membrane. We studied insertion of granules in bovine chromaffin cells using capacitance as a measure of plasma membrane area and fluorescence of a membrane marker FM1-43 as a measure of exocytosis. Intracellular dialysis with [Ca(2+)] (1.5-100 microM) evoked massive exocytosis that was sufficient to double plasma membrane area but did not swell cells. In principle, in the absence of endocytosis, the addition of granule membrane would be anticipated to produce similar increases in the capacitance and FM1-43 fluorescence responses. However, when endocytosis was minimal, the changes in capacitance were markedly larger than the corresponding changes in FM1-43 fluorescence. Moreover, the apparent differences between capacitance and FM1-43 fluorescence changes increased with larger exocytic responses, as more granules fused with the plasma membrane. In experiments in which exocytosis was suppressed, increasing membrane tension by osmotically induced cell swelling increased FM1-43 fluorescence, suggesting that FM1-43 fluorescence is sensitive to changes in the membrane tension. Thus, increasing membrane area through exocytosis does not swell chromaffin cells but may decrease membrane tension.

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“…Therefore, it is unclear whether the lower level of PC is a common feature in secretory vesicles or not. On the contrary, a higher PE content in SG than that in Ms might be specific in the rat parotid gland since the PE level in the secretory vesicles from other species and/or tissues was nearly equal to that in their microsomes2, 25,26,[30][31][32].…”

Section: Physical Propertiesmentioning

confidence: 99%

“…This feature may not be an essential factor for exocytosis since higher PE level was not observed in the other secretory systems2, 25,26,[30][31][32]. However, many investigators have actually demonstrated that PE rich membranes more easily fuse than PC rich membranes in artificial model systems41-43) and an increase in certain phospholipid classes including PE was reported during megamitochondrial formation which may result from the fusion of mitochondria44).…”

Section: Physical Propertiesmentioning

confidence: 99%

Characterization of secretory granular membranes from rat parotid gland: analysis of membrane lipid composition and fluidity.

et al. 1990

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Phospholipid composition of some amine storage granules

Cited by 19 publications

References 34 publications

Purification of age-distinct insulin secretory granules through antigen restriction

Purification of age-distinct insulin secretory granules through antigen restriction

Exocytosis in Bovine Chromaffin Cells: Studies with Patch-Clamp Capacitance and FM1-43 Fluorescence

Characterization of secretory granular membranes from rat parotid gland: analysis of membrane lipid composition and fluidity.

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