Phospholipid metabolism in the mammalian heart

Choy, Patrick C.; Tran, Khai; Hatch, Grant M.; Kroeger, Edwin A.

doi:10.1016/s0163-7827(97)00005-2

Cited by 20 publications

(13 citation statements)

References 134 publications

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“…16). The mechanism of incorporation is unclear, but is probably mediated by the acyl-CoA: lysophosphatidylcholine acyltransferase, which is part of the deacylationreacylation of phospholipids (15,23). There is no evidence for a receptor-mediated regulation of the acyltransferases.…”

Section: Incorporation Ofmentioning

confidence: 99%

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Effects of Norepinephrine and Cardiotrophin-1 on Phospholipase D Activity and Incorporation of Myristic Acid Into Phosphatidylcholine in Rat Heart

2004

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Abstract. The present study is part of a project on phospholipase D (PLD) in cardiac hypertrophy and analyzed effects on PLD activity of two growth stimuli, norepinephrine (NE) and cardiotrophin-1 (CT-1), in incubated rat heart. Phosphatidylcholine (PC) was labeled by 3 Hmyristic acid. PLD produced 3 H-phosphatidylethanol ( 3 H-PEth) from 3 H-PC in the presence of ethanol and maintained a basal formation of 3 H-PEth. Short-term and long-term exposure to NE for 2 or 13 h, respectively, enhanced the formation of 3 H-PEth, which was blocked by prazosin. Long-term pretreatment with NE or CT-1 increased the incorporation of 3 H-myristic acid into PC, which was blocked by atenolol. When the 3 H-PEth formation was expressed as a fraction of 3 H-PC, PLD activity seemingly was unchanged (NE) or markedly reduced (CT-1); the true effects, namely, stimulation by NE and nonresponsiveness towards CT-1, were unraveled by atenolol (NE) or when PLD activity was expressed as 3 H-PEth per ng protein.In conclusion, aadrenoceptor activation increased PLD activity. Long-term treatment with NE (via b-receptors) or CT-1 enhanced the 3 H-myristic acid incorporation into a PC compartment, that was not available for the a -receptor-mediated PLD activation. These results were discussed in regard to cellular mechanisms of cardiac hypertrophy and to the transphosphatidylation assay of PLD.

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Section: Incorporation Ofmentioning

confidence: 99%

“…PLA activation is part of the deacylation-reacylation cycle (15) and thereby is involved in the remodeling of membrane phospholipids, for example, during hypertrophic cell growth.…”

Section: Introductionmentioning

confidence: 99%

Effects of Norepinephrine and Cardiotrophin-1 on Phospholipase D Activity and Incorporation of Myristic Acid Into Phosphatidylcholine in Rat Heart

2004

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“…1 are key components of membrane structure, modulate membrane enzyme activity, and participate in signaling pathways (1)(2)(3). The composition and content of PPL are altered in physiologic and pathologic cellular processes (4 -6).…”

Section: Phospholipids (Ppl)mentioning

confidence: 99%

Separation and Quantitation of Phospholipids and Lysophospholipids by High-Performance Liquid Chromatography

Lesnefsky

Stoll

Minkler

et al. 2000

Analytical Biochemistry

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“…The major phosphodiester components in the mammalian heart are glycerophosphocholine (GPCho) and glycerophosphoethanolamine (GPEth) [34−37]. Phosphomonoesters (PME), such as phosphocholine (PCho) and phosphoethanolamine (PEth), are commonly regarded as phospholipid precursors, whereas PDEs are mainly formed as phospholipid breakdown products [27,37]. The formation and decomposition of PDEs are controlled by the activity of several enzymes, such as phospholipases, lysophospolipases or possibly phosphodiesterases [37].…”

Section: Discussionmentioning

confidence: 99%

“…Phosphomonoesters (PME), such as phosphocholine (PCho) and phosphoethanolamine (PEth), are commonly regarded as phospholipid precursors, whereas PDEs are mainly formed as phospholipid breakdown products [27,37]. The formation and decomposition of PDEs are controlled by the activity of several enzymes, such as phospholipases, lysophospolipases or possibly phosphodiesterases [37]. Several changes in enzyme activity were reported in hypertensive heart disease and heart hypertrophy, but the regulation mechanisms of the interacting enzymes and the resulting metabolic changes remain unclear [37−39].…”

Section: Discussionmentioning

confidence: 99%

31P-MR spectroscopic imaging in hypertensive heart disease

et al. 2006

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Hypertensive heart disease (HHD) causes structural changes (e.g., fibrosis) that result in diastolic and systolic myocardial dysfunction. Alterations of (31)P metabolism and cardiac energy impairments were assessed in patients with HHD by MR spectroscopy (MRS) and correlated with left ventricular systolic function. Thirty-six patients with HHD and 20 healthy controls (mean age 35.2+/-10.7 years) were examined with (31)P-MRS at 1.5 T by using an ECG-gated CSI sequence. Twenty-five patients (mean age 64.3+/-9.3 years) had diastolic dysfunction, but preserved systolic function (HHD-D), whereas 11 patients (62.3+/-11.4 years) suffered from additional impaired systolic function (HHD-S). In both patient groups, the PCr/gamma-ATP ratio was lower than in the controls (controls: 2.07+/-0.17; P<0.001), and in HHD-S was lower than in HHD-D (1.43+/-0.21 vs. 1.65+/-0.25; P=0.012). PCr/gamma-ATP ratios were linearly correlated with LVEF (Pearson's r: 0.39; P=0.025). In the HHD-S group, the PDE/gamma-ATP ratio was significantly lower (0.56+/-0.36) than in the controls (1.14+/-0.42; P=0.001). In contrast to the group of HHD-D patients, whose slightly decreased PCr/gamma-ATP ratios compared to controls may be explained by age differences, the more distinct changes observed in HHD-S patients indicate an altered energy metabolism. The observed metabolic changes were related to functional impairments, as indicated by a reduced LVEF. Reduced PDE/ATP ratios indicate changes in the phospholipid metabolism.

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Phospholipid metabolism in the mammalian heart

Cited by 20 publications

References 134 publications

Effects of Norepinephrine and Cardiotrophin-1 on Phospholipase D Activity and Incorporation of Myristic Acid Into Phosphatidylcholine in Rat Heart

Effects of Norepinephrine and Cardiotrophin-1 on Phospholipase D Activity and Incorporation of Myristic Acid Into Phosphatidylcholine in Rat Heart

Separation and Quantitation of Phospholipids and Lysophospholipids by High-Performance Liquid Chromatography

31P-MR spectroscopic imaging in hypertensive heart disease

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