Phospholipid Metabolism in Vitamin A-deficient Rats

Krause, R. F.; Beamer, Kathryn C.; Plow, Jane H.

doi:10.1093/jn/101.2.161

Cited by 9 publications

(9 citation statements)

References 16 publications

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“…The 3H-AEP lipid found in the mitochondrial fraction was most likely due to an exchange of phosphonolipid between the polysomes and the mitochondria, while the radioactive lipid of the nuclear fraction was probably for the most part the result of mitochondrial contamination. The exchange of phosphonolipid between the microsomes and mitochondria has been demonstrated by other researchers (38) and the exchange of phospholipid has also been reported by many laboratories (41,(43)(44)(45). The pattern of radioactivity found in the present investigations could also indicate the localization of phosphonolipids in the plasma membrane, since plasma membrane fragments are found, for the most part, in the nuclear and polysomal fractions (46).…”

Section: Discussionsupporting

confidence: 86%

2‐Aminoethylphosphonic acid metabolism in the rat

Curley-Joseph

Henderson

1977

Lipids

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Time course studies of the incorporation of radioactive 2-aminoethylphosphonic acid (AEP) into the tissues of rats demonstrated that maximum incorporation into the liver lipids occurred within 12 to 30 hr after injection, compared to 2 to 3 hr for the incorporation of phosphorylethanolamine. Little incorporation of AEP was observed in the other tissues investigated (heart, lung, spleen, adipose, kidney). The AEP was incorporated to the greatest extent into 1,2-diacylglyceryl-aminoethylphosphonate (diacylglyceryl-AEP), the phosphonate analogue of phosphatidylethanolamine, with some incorporation into the lyso derivative. Diacylglycerol-AEP apparently was not further metabolized by the rat; no methylation of diacylglyceryl-AEP to phosphonolecithin was observed. Subcellular fractionation was performed on the livers of rats who received (3)H-AEP 12,30,36, and 48 hr prior to sacrifice. The greatest amount of radioactivity was recovered in the soluble fractions. Lipid extraction was performed on the subcellular fractions, and most of the radioactivity present in the lipids was found in the microsomal fraction, with the next highest recovery in the mitochondrial and nuclear fractions

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Section: Discussionsupporting

confidence: 86%

2‐Aminoethylphosphonic acid metabolism in the rat

Curley-Joseph

Henderson

1977

Lipids

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“…In their study they also indicated that there were not parallel changes in the levels of C16 :21 and C18: 1 fatty acids in these tissues. There have been two other reports in the literature [18,19] concerning fatty acid composition in liver membrane fractions from vitamin A-deficient rats. Krause et al [18] only analysed mitochondrial membranes.…”

Section: Discussionmentioning

confidence: 99%

“…There have been two other reports in the literature [18,19] concerning fatty acid composition in liver membrane fractions from vitamin A-deficient rats. Krause et al [18] only analysed mitochondrial membranes. Vaughan-Mitchell et al [19] reported similar decreases to those seen here in C18: 2 and C20 4 fatty acids.…”

Section: Discussionmentioning

confidence: 99%

Liver microsomal membrane fluidity and lipid characteristics in vitamin A-deficient rats

Hamm

Chan

Wolf

1987

Biochemical Journal

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Rat liver microsomes (microsomal fraction) were isolated from vitamin A-deficient and -sufficient rats and analysed for membrane lipid characteristics. Membrane fluidity was found to be significantly decreased in microsomes from the vitamin A-deficient rats, but not in liposomes prepared from lipid extracts. Microsomes from vitamin A-deficient animals showed a significant decrease in C18:2, omega 6 and an increase in C22:5, omega 6 fatty acids.

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“…Whether supplementation of 18:2 treats vitamin A deficiency has been controversially discussed since 1930 (31,32), when Chidester et alf ound that 18:2 in the diet impairs malfunctions during hypovitaminosis in rats (30). Later, vitamin A deficiency was shown to increase 18:2 levels in liver and other tissues (33,34). Moreover, plenty of evidence suggests a metabolic and functional link between vitamin A and conjugated 18:2, an isomer of 18:2, which interferes with PUFA metabolism (35).…”

Section: Discussionmentioning

confidence: 99%

Vitamin A regulates Akt signaling through the phospholipid fatty acid composition

et al. 2017

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Protein kinases, including the serine/threonine kinase Akt, mediate manifold bioactivities of vitamin A, although the mechanisms behind the sustained kinase activation are diffuse. To investigate the role of cellular lipids as targetable factors in Akt signaling, we combined mass spectrometry-based lipidomics with immunologic detection of Akt (Ser473) phosphorylation. A screening campaign revealed retinol (vitamin A alcohol) and all- retinoic acid (vitamin A acid) (RA) as hits that time-dependently (≥24 h) deplete phosphatidylcholine-bound polyunsaturated fatty acids (PUFA-PCs) from NIH-3T3 mouse fibroblasts while inducing Akt activation (EC ≈ 0.1-1 µM). Other mitogenic and stress-regulated kinases were hardly affected. Organized in a coregulated phospholipid subcluster, PUFA-PCs compensated for the RA-induced loss of cellular PUFA-PCs and diminished Akt activation when supplemented. The counter-regulation of phospholipids and Akt by RA was mimicked by knockdown of lysophosphatidylcholine acyltransferase-3 or the selective retinoid X receptor (RXR) agonist bexarotene and prevented by the selective RXR antagonist Hx531. Treatment of mice with retinol decreased the tissue ratio of PUFA-PC and enhanced basal Akt activation preferentially in brain, which was attributed to astrocytes in dissociated cortical cultures. Together, our findings show that RA regulates the long-term activation of Akt by changes in the phospholipid composition.-Pein, H., Koeberle, S. C., Voelkel, M., Schneider, F., Rossi, A., Thürmer, M., Loeser, K., Sautebin, L., Morrison, H., Werz, O., Koeberle, A. Vitamin A regulates Akt signaling through the phospholipid fatty acid composition.

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Phospholipid Metabolism in Vitamin A-deficient Rats

Cited by 9 publications

References 16 publications

2‐Aminoethylphosphonic acid metabolism in the rat

2‐Aminoethylphosphonic acid metabolism in the rat

Liver microsomal membrane fluidity and lipid characteristics in vitamin A-deficient rats

Vitamin A regulates Akt signaling through the phospholipid fatty acid composition

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