Phospholipids. 3. On the chromatographic separation of glycerophospholipids

Chromatographic separation of brain lipids: cerebroside and sulphatide

“…As further experiments were planned to study the chromatographic behaviour of the ethanolamineand serine-containing phospholipids, it was also considered desirable to remove the proteolipid protein. The crude lipid extract of brain (stage 1) was therefore subjected to the following successive treatments: (a) removal of water-soluble components by solvent partition (stage 2); (b) precipitation of proteolipid protein by heat treatment (stage 3); (c) passage through a cellulose column (stage 4), according to Lea & Rhodes (1953). At each stage, analyses were made for galactose, sphingosine, total N, amino N, total P, ester and cholesterol.…”

The incorporation of the phosphate esters of N-substituted aminoethanols into the phospholipids of brain and liver

“…2(a) was identified as phosphatidyl-2-dimethylaminoethanol from the following criteria: (1) When the material in the radioactive peak from the alumina column was mixed with synthetic distearoylphosphatidyl-2-dimethylaminoethanol (kindly provided by Professor E. Baer) and resubjected to chromatography on alumina, the elution of the synthetic material coincided with that of the radioactive lipid. (2) Chromatography of the total lipid extract on silicic acid paper in the chloroform-methanol solvent of Lea, Rhodes & Stoll (1955) or the diisobutyl ketone-acetic acid-water solvent of Marinetti (1962) gave only one significantly radioactive spot. Thin-layer chromatography on silica gel G (Merck) gave similar results.…”

Platelet Phosphatides: Their Separation, Identification, and Clotting Activity1

“…Three solvent systems were used. The first was based on the method of Lea, Rhodes and Stoll (52). A round chromatography jar, 25 X 45 cm., was used.3 One hundred micrograms of platelet phospholipid in chloroform solution was applied to the starting line of silicic acid-impregnated Whatman No.…”